Cargando…

Structural basis of the signalling through a bacterial membrane receptor HasR deciphered by an integrative approach

Bacteria use diverse signalling pathways to adapt gene expression to external stimuli. In Gram-negative bacteria, the binding of scarce nutrients to membrane transporters triggers a signalling process that up-regulates the expression of genes of various functions, from uptake of nutrient to producti...

Descripción completa

Detalles Bibliográficos
Autores principales: Wojtowicz, Halina, Prochnicka-Chalufour, Ada, deAmorim, Gisele Cardoso, Roudenko, Olga, Simenel, Catherine, Malki, Idir, Pehau-Arnaudet, Gérard, Gubellini, Francesca, Koutsioubas, Alexandros, Pérez, Javier, Delepelaire, Philippe, Delepierre, Muriel, Fronzes, Rémi, Izadi-Pruneyre, Nadia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4941744/
https://www.ncbi.nlm.nih.gov/pubmed/27208170
http://dx.doi.org/10.1042/BCJ20160131
_version_ 1782442344796127232
author Wojtowicz, Halina
Prochnicka-Chalufour, Ada
deAmorim, Gisele Cardoso
Roudenko, Olga
Simenel, Catherine
Malki, Idir
Pehau-Arnaudet, Gérard
Gubellini, Francesca
Koutsioubas, Alexandros
Pérez, Javier
Delepelaire, Philippe
Delepierre, Muriel
Fronzes, Rémi
Izadi-Pruneyre, Nadia
author_facet Wojtowicz, Halina
Prochnicka-Chalufour, Ada
deAmorim, Gisele Cardoso
Roudenko, Olga
Simenel, Catherine
Malki, Idir
Pehau-Arnaudet, Gérard
Gubellini, Francesca
Koutsioubas, Alexandros
Pérez, Javier
Delepelaire, Philippe
Delepierre, Muriel
Fronzes, Rémi
Izadi-Pruneyre, Nadia
author_sort Wojtowicz, Halina
collection PubMed
description Bacteria use diverse signalling pathways to adapt gene expression to external stimuli. In Gram-negative bacteria, the binding of scarce nutrients to membrane transporters triggers a signalling process that up-regulates the expression of genes of various functions, from uptake of nutrient to production of virulence factors. Although proteins involved in this process have been identified, signal transduction through this family of transporters is not well understood. In the present study, using an integrative approach (EM, SAXS, X-ray crystallography and NMR), we have studied the structure of the haem transporter HasR captured in two stages of the signalling process, i.e. before and after the arrival of signalling activators (haem and its carrier protein). We show for the first time that the HasR domain responsible for signal transfer: (i) is highly flexible in two stages of signalling; (ii) extends into the periplasm at approximately 70–90 Å (1 Å=0.1 nm) from the HasR β-barrel; and (iii) exhibits local conformational changes in response to the arrival of signalling activators. These features would favour the signal transfer from HasR to its cytoplasmic membrane partners.
format Online
Article
Text
id pubmed-4941744
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Portland Press Ltd.
record_format MEDLINE/PubMed
spelling pubmed-49417442016-07-22 Structural basis of the signalling through a bacterial membrane receptor HasR deciphered by an integrative approach Wojtowicz, Halina Prochnicka-Chalufour, Ada deAmorim, Gisele Cardoso Roudenko, Olga Simenel, Catherine Malki, Idir Pehau-Arnaudet, Gérard Gubellini, Francesca Koutsioubas, Alexandros Pérez, Javier Delepelaire, Philippe Delepierre, Muriel Fronzes, Rémi Izadi-Pruneyre, Nadia Biochem J Research Articles Bacteria use diverse signalling pathways to adapt gene expression to external stimuli. In Gram-negative bacteria, the binding of scarce nutrients to membrane transporters triggers a signalling process that up-regulates the expression of genes of various functions, from uptake of nutrient to production of virulence factors. Although proteins involved in this process have been identified, signal transduction through this family of transporters is not well understood. In the present study, using an integrative approach (EM, SAXS, X-ray crystallography and NMR), we have studied the structure of the haem transporter HasR captured in two stages of the signalling process, i.e. before and after the arrival of signalling activators (haem and its carrier protein). We show for the first time that the HasR domain responsible for signal transfer: (i) is highly flexible in two stages of signalling; (ii) extends into the periplasm at approximately 70–90 Å (1 Å=0.1 nm) from the HasR β-barrel; and (iii) exhibits local conformational changes in response to the arrival of signalling activators. These features would favour the signal transfer from HasR to its cytoplasmic membrane partners. Portland Press Ltd. 2016-07-12 2016-07-15 /pmc/articles/PMC4941744/ /pubmed/27208170 http://dx.doi.org/10.1042/BCJ20160131 Text en © 2016 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution Licence 4.0 (CC BY-NC-ND) (http://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Research Articles
Wojtowicz, Halina
Prochnicka-Chalufour, Ada
deAmorim, Gisele Cardoso
Roudenko, Olga
Simenel, Catherine
Malki, Idir
Pehau-Arnaudet, Gérard
Gubellini, Francesca
Koutsioubas, Alexandros
Pérez, Javier
Delepelaire, Philippe
Delepierre, Muriel
Fronzes, Rémi
Izadi-Pruneyre, Nadia
Structural basis of the signalling through a bacterial membrane receptor HasR deciphered by an integrative approach
title Structural basis of the signalling through a bacterial membrane receptor HasR deciphered by an integrative approach
title_full Structural basis of the signalling through a bacterial membrane receptor HasR deciphered by an integrative approach
title_fullStr Structural basis of the signalling through a bacterial membrane receptor HasR deciphered by an integrative approach
title_full_unstemmed Structural basis of the signalling through a bacterial membrane receptor HasR deciphered by an integrative approach
title_short Structural basis of the signalling through a bacterial membrane receptor HasR deciphered by an integrative approach
title_sort structural basis of the signalling through a bacterial membrane receptor hasr deciphered by an integrative approach
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4941744/
https://www.ncbi.nlm.nih.gov/pubmed/27208170
http://dx.doi.org/10.1042/BCJ20160131
work_keys_str_mv AT wojtowiczhalina structuralbasisofthesignallingthroughabacterialmembranereceptorhasrdecipheredbyanintegrativeapproach
AT prochnickachalufourada structuralbasisofthesignallingthroughabacterialmembranereceptorhasrdecipheredbyanintegrativeapproach
AT deamorimgiselecardoso structuralbasisofthesignallingthroughabacterialmembranereceptorhasrdecipheredbyanintegrativeapproach
AT roudenkoolga structuralbasisofthesignallingthroughabacterialmembranereceptorhasrdecipheredbyanintegrativeapproach
AT simenelcatherine structuralbasisofthesignallingthroughabacterialmembranereceptorhasrdecipheredbyanintegrativeapproach
AT malkiidir structuralbasisofthesignallingthroughabacterialmembranereceptorhasrdecipheredbyanintegrativeapproach
AT pehauarnaudetgerard structuralbasisofthesignallingthroughabacterialmembranereceptorhasrdecipheredbyanintegrativeapproach
AT gubellinifrancesca structuralbasisofthesignallingthroughabacterialmembranereceptorhasrdecipheredbyanintegrativeapproach
AT koutsioubasalexandros structuralbasisofthesignallingthroughabacterialmembranereceptorhasrdecipheredbyanintegrativeapproach
AT perezjavier structuralbasisofthesignallingthroughabacterialmembranereceptorhasrdecipheredbyanintegrativeapproach
AT delepelairephilippe structuralbasisofthesignallingthroughabacterialmembranereceptorhasrdecipheredbyanintegrativeapproach
AT delepierremuriel structuralbasisofthesignallingthroughabacterialmembranereceptorhasrdecipheredbyanintegrativeapproach
AT fronzesremi structuralbasisofthesignallingthroughabacterialmembranereceptorhasrdecipheredbyanintegrativeapproach
AT izadipruneyrenadia structuralbasisofthesignallingthroughabacterialmembranereceptorhasrdecipheredbyanintegrativeapproach