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Quantitative Super-Resolution Imaging with qPAINT using Transient Binding Analysis
Current super-resolution techniques offer unprecedented spatial resolution, but quantitative counting of spatially unresolvable molecules remains challenging. Here, we use the programmable and specific binding of dye-labeled DNA probes to count integer numbers of targets. This method, called quantit...
Autores principales: | Jungmann, Ralf, Avendaño, Maier S, Dai, Mingjie, Woehrstein, Johannes B, Agasti, Sarit S, Feiger, Zachary, Rodal, Avital, Yin, Peng |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4941813/ https://www.ncbi.nlm.nih.gov/pubmed/27018580 http://dx.doi.org/10.1038/nmeth.3804 |
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