In-vivo imaging of dendritic pruning in dentate granule cells

We longitudinally imaged the developing dendrites of adult-born mouse dentate granule cells (DGCs) in vivo and found that they underwent over-branching and pruning. Exposure to an enriched environment (EE) and constraining dendritic growth by disrupting Wnt signaling led to increased branch addition...

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Autores principales: Gonçalves, J. Tiago, Bloyd, Cooper W., Shtrahman, Matthew, Johnston, Stephen T., Schafer, Simon T., Parylak, Sarah L., Tran, Thanh, Chang, Tina, Gage, Fred H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4941946/
https://www.ncbi.nlm.nih.gov/pubmed/27135217
http://dx.doi.org/10.1038/nn.4301
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author Gonçalves, J. Tiago
Bloyd, Cooper W.
Shtrahman, Matthew
Johnston, Stephen T.
Schafer, Simon T.
Parylak, Sarah L.
Tran, Thanh
Chang, Tina
Gage, Fred H.
author_facet Gonçalves, J. Tiago
Bloyd, Cooper W.
Shtrahman, Matthew
Johnston, Stephen T.
Schafer, Simon T.
Parylak, Sarah L.
Tran, Thanh
Chang, Tina
Gage, Fred H.
author_sort Gonçalves, J. Tiago
collection PubMed
description We longitudinally imaged the developing dendrites of adult-born mouse dentate granule cells (DGCs) in vivo and found that they underwent over-branching and pruning. Exposure to an enriched environment (EE) and constraining dendritic growth by disrupting Wnt signaling led to increased branch addition and accelerated growth, which were, however, counteracted by earlier and more extensive pruning. Our results indicate that pruning is regulated in a homeostatic fashion to oppose excessive branching and promote a similar dendrite structure in DGCs.
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spelling pubmed-49419462016-11-02 In-vivo imaging of dendritic pruning in dentate granule cells Gonçalves, J. Tiago Bloyd, Cooper W. Shtrahman, Matthew Johnston, Stephen T. Schafer, Simon T. Parylak, Sarah L. Tran, Thanh Chang, Tina Gage, Fred H. Nat Neurosci Article We longitudinally imaged the developing dendrites of adult-born mouse dentate granule cells (DGCs) in vivo and found that they underwent over-branching and pruning. Exposure to an enriched environment (EE) and constraining dendritic growth by disrupting Wnt signaling led to increased branch addition and accelerated growth, which were, however, counteracted by earlier and more extensive pruning. Our results indicate that pruning is regulated in a homeostatic fashion to oppose excessive branching and promote a similar dendrite structure in DGCs. 2016-05-02 2016-06 /pmc/articles/PMC4941946/ /pubmed/27135217 http://dx.doi.org/10.1038/nn.4301 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Gonçalves, J. Tiago
Bloyd, Cooper W.
Shtrahman, Matthew
Johnston, Stephen T.
Schafer, Simon T.
Parylak, Sarah L.
Tran, Thanh
Chang, Tina
Gage, Fred H.
In-vivo imaging of dendritic pruning in dentate granule cells
title In-vivo imaging of dendritic pruning in dentate granule cells
title_full In-vivo imaging of dendritic pruning in dentate granule cells
title_fullStr In-vivo imaging of dendritic pruning in dentate granule cells
title_full_unstemmed In-vivo imaging of dendritic pruning in dentate granule cells
title_short In-vivo imaging of dendritic pruning in dentate granule cells
title_sort in-vivo imaging of dendritic pruning in dentate granule cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4941946/
https://www.ncbi.nlm.nih.gov/pubmed/27135217
http://dx.doi.org/10.1038/nn.4301
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