The Equilibria of Diosgenin–Phosphatidylcholine and Diosgenin–Cholesterol in Monolayers at the Air/Water Interface

Diosgenin (Dio) has shown many treatment properties, but the most important property is cytotoxic activity in cancer cells. In this study, we investigated monolayers of Dio, cholesterol (Ch), and phosphatidylcholine (PC) at the air/water interface. The measurements were carried with a Langmuir Teflo...

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Detalles Bibliográficos
Autores principales: Janicka, Katarzyna, Jastrzebska, Izabella, Petelska, Aneta Dorota
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4942497/
https://www.ncbi.nlm.nih.gov/pubmed/27350149
http://dx.doi.org/10.1007/s00232-016-9914-1
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author Janicka, Katarzyna
Jastrzebska, Izabella
Petelska, Aneta Dorota
author_facet Janicka, Katarzyna
Jastrzebska, Izabella
Petelska, Aneta Dorota
author_sort Janicka, Katarzyna
collection PubMed
description Diosgenin (Dio) has shown many treatment properties, but the most important property is cytotoxic activity in cancer cells. In this study, we investigated monolayers of Dio, cholesterol (Ch), and phosphatidylcholine (PC) at the air/water interface. The measurements were carried with a Langmuir Teflon trough and a Nima 9000 tensiometer program. The surface tension values of pure and mixed monolayers were used to calculate π–A isotherms and determine molecular surface areas. We were able to demonstrate the formation of complexes between Dio and PC and Dio and Ch molecules also. We considered the equilibrium between individual components and the formed complexes. In addition, we established that diosgenin and the lipids formed highly stable 1:1 complexes.
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spelling pubmed-49424972016-07-26 The Equilibria of Diosgenin–Phosphatidylcholine and Diosgenin–Cholesterol in Monolayers at the Air/Water Interface Janicka, Katarzyna Jastrzebska, Izabella Petelska, Aneta Dorota J Membr Biol Article Diosgenin (Dio) has shown many treatment properties, but the most important property is cytotoxic activity in cancer cells. In this study, we investigated monolayers of Dio, cholesterol (Ch), and phosphatidylcholine (PC) at the air/water interface. The measurements were carried with a Langmuir Teflon trough and a Nima 9000 tensiometer program. The surface tension values of pure and mixed monolayers were used to calculate π–A isotherms and determine molecular surface areas. We were able to demonstrate the formation of complexes between Dio and PC and Dio and Ch molecules also. We considered the equilibrium between individual components and the formed complexes. In addition, we established that diosgenin and the lipids formed highly stable 1:1 complexes. Springer US 2016-06-27 2016 /pmc/articles/PMC4942497/ /pubmed/27350149 http://dx.doi.org/10.1007/s00232-016-9914-1 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Janicka, Katarzyna
Jastrzebska, Izabella
Petelska, Aneta Dorota
The Equilibria of Diosgenin–Phosphatidylcholine and Diosgenin–Cholesterol in Monolayers at the Air/Water Interface
title The Equilibria of Diosgenin–Phosphatidylcholine and Diosgenin–Cholesterol in Monolayers at the Air/Water Interface
title_full The Equilibria of Diosgenin–Phosphatidylcholine and Diosgenin–Cholesterol in Monolayers at the Air/Water Interface
title_fullStr The Equilibria of Diosgenin–Phosphatidylcholine and Diosgenin–Cholesterol in Monolayers at the Air/Water Interface
title_full_unstemmed The Equilibria of Diosgenin–Phosphatidylcholine and Diosgenin–Cholesterol in Monolayers at the Air/Water Interface
title_short The Equilibria of Diosgenin–Phosphatidylcholine and Diosgenin–Cholesterol in Monolayers at the Air/Water Interface
title_sort equilibria of diosgenin–phosphatidylcholine and diosgenin–cholesterol in monolayers at the air/water interface
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4942497/
https://www.ncbi.nlm.nih.gov/pubmed/27350149
http://dx.doi.org/10.1007/s00232-016-9914-1
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