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3′-UTR engineering to improve soluble expression and fine-tuning of activity of cascade enzymes in Escherichia coli

3′-Untranslated region (3′UTR) engineering was investigated to improve solubility of heterologous proteins (e.g., Baeyer-Villiger monooxygenases (BVMOs)) in Escherichia coli. Insertion of gene fragments containing putative RNase E recognition sites into the 3′UTR of the BVMO genes led to the reducti...

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Detalles Bibliográficos
Autores principales:	Song, Ji-Won, Woo, Ji-Min, Jung, Gyoo Yeol, Bornscheuer, Uwe T., Park, Jin-Byung
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4942690/ https://www.ncbi.nlm.nih.gov/pubmed/27406241 http://dx.doi.org/10.1038/srep29406

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