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Correlates of Immunity to Influenza as Determined by Challenge of Children with Live, Attenuated Influenza Vaccine
Background. The efficacy of live, attenuated live attenuated influenza vaccine(LAIV) and inactivated influenza vaccine(IIV) is poorly explained by either single or composite immune responses to vaccination. Protective biomarkers were therefore studied in response to LAIV or IIV followed by LAIV chal...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Oxford University Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4943547/ https://www.ncbi.nlm.nih.gov/pubmed/27419180 http://dx.doi.org/10.1093/ofid/ofw108 |
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author | Wright, Peter F. Hoen, Anne G. Ilyushina, Natalia A. Brown, Eric P. Ackerman, Margaret E. Wieland-Alter, Wendy Connor, Ruth I. Jegaskanda, Sinthujan Rosenberg-Hasson, Yael Haynes, Brenda C. Luke, Catherine J. Subbarao, Kanta Treanor, John J. |
author_facet | Wright, Peter F. Hoen, Anne G. Ilyushina, Natalia A. Brown, Eric P. Ackerman, Margaret E. Wieland-Alter, Wendy Connor, Ruth I. Jegaskanda, Sinthujan Rosenberg-Hasson, Yael Haynes, Brenda C. Luke, Catherine J. Subbarao, Kanta Treanor, John J. |
author_sort | Wright, Peter F. |
collection | PubMed |
description | Background. The efficacy of live, attenuated live attenuated influenza vaccine(LAIV) and inactivated influenza vaccine(IIV) is poorly explained by either single or composite immune responses to vaccination. Protective biomarkers were therefore studied in response to LAIV or IIV followed by LAIV challenge in children. Methods. Serum and mucosal responses to LAIV or IIV were analyzed using immunologic assays to assess both quantitative and functional responses. Cytokines and chemokines were measured in nasal washes collected before vaccination, on days 2, 4, and 7 after initial LAIV, and again after LAIV challenge using a 63-multiplex Luminex panel. Results. Patterns of immunity induced by LAIV and IIV were significantly different. Serum responses induced by IIV, including hemagglutination inhibition, did not correlate with detection or quantitation of LAIV on subsequent challenge. Modalities that induced sterilizing immunity seen after LAIV challenge could not be defined by any measurements of mucosal or serum antibodies induced by the initial LAIV immunization. No single cytokine or chemokine was predictive of protection. Conclusions. The mechanism of protective immunity observed after LAIV could not be defined, and traditional measurements of immunity to IIV did not correlate with protection against an LAIV challenge. |
format | Online Article Text |
id | pubmed-4943547 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-49435472016-07-14 Correlates of Immunity to Influenza as Determined by Challenge of Children with Live, Attenuated Influenza Vaccine Wright, Peter F. Hoen, Anne G. Ilyushina, Natalia A. Brown, Eric P. Ackerman, Margaret E. Wieland-Alter, Wendy Connor, Ruth I. Jegaskanda, Sinthujan Rosenberg-Hasson, Yael Haynes, Brenda C. Luke, Catherine J. Subbarao, Kanta Treanor, John J. Open Forum Infect Dis Major Articles Background. The efficacy of live, attenuated live attenuated influenza vaccine(LAIV) and inactivated influenza vaccine(IIV) is poorly explained by either single or composite immune responses to vaccination. Protective biomarkers were therefore studied in response to LAIV or IIV followed by LAIV challenge in children. Methods. Serum and mucosal responses to LAIV or IIV were analyzed using immunologic assays to assess both quantitative and functional responses. Cytokines and chemokines were measured in nasal washes collected before vaccination, on days 2, 4, and 7 after initial LAIV, and again after LAIV challenge using a 63-multiplex Luminex panel. Results. Patterns of immunity induced by LAIV and IIV were significantly different. Serum responses induced by IIV, including hemagglutination inhibition, did not correlate with detection or quantitation of LAIV on subsequent challenge. Modalities that induced sterilizing immunity seen after LAIV challenge could not be defined by any measurements of mucosal or serum antibodies induced by the initial LAIV immunization. No single cytokine or chemokine was predictive of protection. Conclusions. The mechanism of protective immunity observed after LAIV could not be defined, and traditional measurements of immunity to IIV did not correlate with protection against an LAIV challenge. Oxford University Press 2016-05-23 /pmc/articles/PMC4943547/ /pubmed/27419180 http://dx.doi.org/10.1093/ofid/ofw108 Text en © The Author 2016. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com. |
spellingShingle | Major Articles Wright, Peter F. Hoen, Anne G. Ilyushina, Natalia A. Brown, Eric P. Ackerman, Margaret E. Wieland-Alter, Wendy Connor, Ruth I. Jegaskanda, Sinthujan Rosenberg-Hasson, Yael Haynes, Brenda C. Luke, Catherine J. Subbarao, Kanta Treanor, John J. Correlates of Immunity to Influenza as Determined by Challenge of Children with Live, Attenuated Influenza Vaccine |
title | Correlates of Immunity to Influenza as Determined by Challenge of Children with Live, Attenuated Influenza Vaccine |
title_full | Correlates of Immunity to Influenza as Determined by Challenge of Children with Live, Attenuated Influenza Vaccine |
title_fullStr | Correlates of Immunity to Influenza as Determined by Challenge of Children with Live, Attenuated Influenza Vaccine |
title_full_unstemmed | Correlates of Immunity to Influenza as Determined by Challenge of Children with Live, Attenuated Influenza Vaccine |
title_short | Correlates of Immunity to Influenza as Determined by Challenge of Children with Live, Attenuated Influenza Vaccine |
title_sort | correlates of immunity to influenza as determined by challenge of children with live, attenuated influenza vaccine |
topic | Major Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4943547/ https://www.ncbi.nlm.nih.gov/pubmed/27419180 http://dx.doi.org/10.1093/ofid/ofw108 |
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