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Efficacy Assessment of Nucleic Acid Decontamination Reagents Used in Molecular Diagnostic Laboratories

The occurrence of nucleic acid cross contamination in the laboratory resulting in false positive results of diagnostic samples is seriously problematic. Despite precautions to minimize or even avoid nucleic acid cross contaminations, it may appear anyway. Until now, no standardized strategy is avail...

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Autores principales: Fischer, Melina, Renevey, Nathalie, Thür, Barbara, Hoffmann, Donata, Beer, Martin, Hoffmann, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4943653/
https://www.ncbi.nlm.nih.gov/pubmed/27410228
http://dx.doi.org/10.1371/journal.pone.0159274
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author Fischer, Melina
Renevey, Nathalie
Thür, Barbara
Hoffmann, Donata
Beer, Martin
Hoffmann, Bernd
author_facet Fischer, Melina
Renevey, Nathalie
Thür, Barbara
Hoffmann, Donata
Beer, Martin
Hoffmann, Bernd
author_sort Fischer, Melina
collection PubMed
description The occurrence of nucleic acid cross contamination in the laboratory resulting in false positive results of diagnostic samples is seriously problematic. Despite precautions to minimize or even avoid nucleic acid cross contaminations, it may appear anyway. Until now, no standardized strategy is available to evaluate the efficacy of commercially offered decontamination reagents. Therefore, a protocol for the reliable determination of nucleic acid decontamination efficacy using highly standardized solution and surface tests was established and validated. All tested sodium hypochlorite-based reagents proved to be highly efficient in nucleic acid decontamination even after short reaction times. For DNA Away, a sodium hydroxide-based decontamination product, dose- and time-dependent effectiveness was ascertained. For two other commercial decontamination reagents, the phosphoric acid-based DNA Remover and the non-enzymatic reagent DNA-ExitusPlus(™) IF, no reduction of amplifiable DNA/RNA was observed. In conclusion, a simple test procedure for evaluation of the elimination efficacy of decontamination reagents against amplifiable nucleic acid is presented.
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spelling pubmed-49436532016-08-01 Efficacy Assessment of Nucleic Acid Decontamination Reagents Used in Molecular Diagnostic Laboratories Fischer, Melina Renevey, Nathalie Thür, Barbara Hoffmann, Donata Beer, Martin Hoffmann, Bernd PLoS One Research Article The occurrence of nucleic acid cross contamination in the laboratory resulting in false positive results of diagnostic samples is seriously problematic. Despite precautions to minimize or even avoid nucleic acid cross contaminations, it may appear anyway. Until now, no standardized strategy is available to evaluate the efficacy of commercially offered decontamination reagents. Therefore, a protocol for the reliable determination of nucleic acid decontamination efficacy using highly standardized solution and surface tests was established and validated. All tested sodium hypochlorite-based reagents proved to be highly efficient in nucleic acid decontamination even after short reaction times. For DNA Away, a sodium hydroxide-based decontamination product, dose- and time-dependent effectiveness was ascertained. For two other commercial decontamination reagents, the phosphoric acid-based DNA Remover and the non-enzymatic reagent DNA-ExitusPlus(™) IF, no reduction of amplifiable DNA/RNA was observed. In conclusion, a simple test procedure for evaluation of the elimination efficacy of decontamination reagents against amplifiable nucleic acid is presented. Public Library of Science 2016-07-13 /pmc/articles/PMC4943653/ /pubmed/27410228 http://dx.doi.org/10.1371/journal.pone.0159274 Text en © 2016 Fischer et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Fischer, Melina
Renevey, Nathalie
Thür, Barbara
Hoffmann, Donata
Beer, Martin
Hoffmann, Bernd
Efficacy Assessment of Nucleic Acid Decontamination Reagents Used in Molecular Diagnostic Laboratories
title Efficacy Assessment of Nucleic Acid Decontamination Reagents Used in Molecular Diagnostic Laboratories
title_full Efficacy Assessment of Nucleic Acid Decontamination Reagents Used in Molecular Diagnostic Laboratories
title_fullStr Efficacy Assessment of Nucleic Acid Decontamination Reagents Used in Molecular Diagnostic Laboratories
title_full_unstemmed Efficacy Assessment of Nucleic Acid Decontamination Reagents Used in Molecular Diagnostic Laboratories
title_short Efficacy Assessment of Nucleic Acid Decontamination Reagents Used in Molecular Diagnostic Laboratories
title_sort efficacy assessment of nucleic acid decontamination reagents used in molecular diagnostic laboratories
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4943653/
https://www.ncbi.nlm.nih.gov/pubmed/27410228
http://dx.doi.org/10.1371/journal.pone.0159274
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