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The Regenerative Potential of Female Skeletal Muscle upon Hypobaric Hypoxic Exposure
Aim: The aim of this study was to determine whether a 14-day trekking expeditions, in high altitude hypoxic environment, triggers redox disturbance at the level of satellite cells (adult stem cells) in young women. Methods: We collected muscle biopsies from Vastus Lateralis muscle for both single fi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4943944/ https://www.ncbi.nlm.nih.gov/pubmed/27471475 http://dx.doi.org/10.3389/fphys.2016.00303 |
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author | Mancinelli, Rosa Di Filippo, Ester S. Verratti, Vittore Fulle, Stefania Toniolo, Luana Reggiani, Carlo Pietrangelo, Tiziana |
author_facet | Mancinelli, Rosa Di Filippo, Ester S. Verratti, Vittore Fulle, Stefania Toniolo, Luana Reggiani, Carlo Pietrangelo, Tiziana |
author_sort | Mancinelli, Rosa |
collection | PubMed |
description | Aim: The aim of this study was to determine whether a 14-day trekking expeditions, in high altitude hypoxic environment, triggers redox disturbance at the level of satellite cells (adult stem cells) in young women. Methods: We collected muscle biopsies from Vastus Lateralis muscle for both single fiber analysis and satellite cells isolation. The samples collected before (PRE-Hypoxia) and after (POST-Hypoxia) the trekking in the Himalayas were compared. Satellite cells were investigated for oxidative stress (oxidant production, antioxidant enzyme activity, and lipid damage), mitochondrial potential variation, gene profile of HIF, and myogenic transcription factors (Pax7, MyoD, myogenin), and miRNA expression (miR-1, miR-133, miR-206). Results: The nuclear domain analysis showed a significant fusion and consequent reduction of the Pax7(+) satellite cells in the single mature fibers. The POST-Hypoxia myoblasts obtained by two out of six volunteers showed high superoxide anion production and lipid peroxidation along with impaired dismutase and catalase and mitochondrial potential. The transcription profile and miRNA expression were different for oxidized and non-oxidized cells. Conclusions: The present study supports the phenomenon of hypobaric-hypoxia-induced oxidative stress and its role in the impairment of the regenerative capacity of satellite cells derived from the V. Lateralis muscle of young adult female subjects. |
format | Online Article Text |
id | pubmed-4943944 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-49439442016-07-28 The Regenerative Potential of Female Skeletal Muscle upon Hypobaric Hypoxic Exposure Mancinelli, Rosa Di Filippo, Ester S. Verratti, Vittore Fulle, Stefania Toniolo, Luana Reggiani, Carlo Pietrangelo, Tiziana Front Physiol Physiology Aim: The aim of this study was to determine whether a 14-day trekking expeditions, in high altitude hypoxic environment, triggers redox disturbance at the level of satellite cells (adult stem cells) in young women. Methods: We collected muscle biopsies from Vastus Lateralis muscle for both single fiber analysis and satellite cells isolation. The samples collected before (PRE-Hypoxia) and after (POST-Hypoxia) the trekking in the Himalayas were compared. Satellite cells were investigated for oxidative stress (oxidant production, antioxidant enzyme activity, and lipid damage), mitochondrial potential variation, gene profile of HIF, and myogenic transcription factors (Pax7, MyoD, myogenin), and miRNA expression (miR-1, miR-133, miR-206). Results: The nuclear domain analysis showed a significant fusion and consequent reduction of the Pax7(+) satellite cells in the single mature fibers. The POST-Hypoxia myoblasts obtained by two out of six volunteers showed high superoxide anion production and lipid peroxidation along with impaired dismutase and catalase and mitochondrial potential. The transcription profile and miRNA expression were different for oxidized and non-oxidized cells. Conclusions: The present study supports the phenomenon of hypobaric-hypoxia-induced oxidative stress and its role in the impairment of the regenerative capacity of satellite cells derived from the V. Lateralis muscle of young adult female subjects. Frontiers Media S.A. 2016-07-14 /pmc/articles/PMC4943944/ /pubmed/27471475 http://dx.doi.org/10.3389/fphys.2016.00303 Text en Copyright © 2016 Mancinelli, Di Filippo, Verratti, Fulle, Toniolo, Reggiani and Pietrangelo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Mancinelli, Rosa Di Filippo, Ester S. Verratti, Vittore Fulle, Stefania Toniolo, Luana Reggiani, Carlo Pietrangelo, Tiziana The Regenerative Potential of Female Skeletal Muscle upon Hypobaric Hypoxic Exposure |
title | The Regenerative Potential of Female Skeletal Muscle upon Hypobaric Hypoxic Exposure |
title_full | The Regenerative Potential of Female Skeletal Muscle upon Hypobaric Hypoxic Exposure |
title_fullStr | The Regenerative Potential of Female Skeletal Muscle upon Hypobaric Hypoxic Exposure |
title_full_unstemmed | The Regenerative Potential of Female Skeletal Muscle upon Hypobaric Hypoxic Exposure |
title_short | The Regenerative Potential of Female Skeletal Muscle upon Hypobaric Hypoxic Exposure |
title_sort | regenerative potential of female skeletal muscle upon hypobaric hypoxic exposure |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4943944/ https://www.ncbi.nlm.nih.gov/pubmed/27471475 http://dx.doi.org/10.3389/fphys.2016.00303 |
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