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The Regenerative Potential of Female Skeletal Muscle upon Hypobaric Hypoxic Exposure

Aim: The aim of this study was to determine whether a 14-day trekking expeditions, in high altitude hypoxic environment, triggers redox disturbance at the level of satellite cells (adult stem cells) in young women. Methods: We collected muscle biopsies from Vastus Lateralis muscle for both single fi...

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Autores principales: Mancinelli, Rosa, Di Filippo, Ester S., Verratti, Vittore, Fulle, Stefania, Toniolo, Luana, Reggiani, Carlo, Pietrangelo, Tiziana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4943944/
https://www.ncbi.nlm.nih.gov/pubmed/27471475
http://dx.doi.org/10.3389/fphys.2016.00303
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author Mancinelli, Rosa
Di Filippo, Ester S.
Verratti, Vittore
Fulle, Stefania
Toniolo, Luana
Reggiani, Carlo
Pietrangelo, Tiziana
author_facet Mancinelli, Rosa
Di Filippo, Ester S.
Verratti, Vittore
Fulle, Stefania
Toniolo, Luana
Reggiani, Carlo
Pietrangelo, Tiziana
author_sort Mancinelli, Rosa
collection PubMed
description Aim: The aim of this study was to determine whether a 14-day trekking expeditions, in high altitude hypoxic environment, triggers redox disturbance at the level of satellite cells (adult stem cells) in young women. Methods: We collected muscle biopsies from Vastus Lateralis muscle for both single fiber analysis and satellite cells isolation. The samples collected before (PRE-Hypoxia) and after (POST-Hypoxia) the trekking in the Himalayas were compared. Satellite cells were investigated for oxidative stress (oxidant production, antioxidant enzyme activity, and lipid damage), mitochondrial potential variation, gene profile of HIF, and myogenic transcription factors (Pax7, MyoD, myogenin), and miRNA expression (miR-1, miR-133, miR-206). Results: The nuclear domain analysis showed a significant fusion and consequent reduction of the Pax7(+) satellite cells in the single mature fibers. The POST-Hypoxia myoblasts obtained by two out of six volunteers showed high superoxide anion production and lipid peroxidation along with impaired dismutase and catalase and mitochondrial potential. The transcription profile and miRNA expression were different for oxidized and non-oxidized cells. Conclusions: The present study supports the phenomenon of hypobaric-hypoxia-induced oxidative stress and its role in the impairment of the regenerative capacity of satellite cells derived from the V. Lateralis muscle of young adult female subjects.
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spelling pubmed-49439442016-07-28 The Regenerative Potential of Female Skeletal Muscle upon Hypobaric Hypoxic Exposure Mancinelli, Rosa Di Filippo, Ester S. Verratti, Vittore Fulle, Stefania Toniolo, Luana Reggiani, Carlo Pietrangelo, Tiziana Front Physiol Physiology Aim: The aim of this study was to determine whether a 14-day trekking expeditions, in high altitude hypoxic environment, triggers redox disturbance at the level of satellite cells (adult stem cells) in young women. Methods: We collected muscle biopsies from Vastus Lateralis muscle for both single fiber analysis and satellite cells isolation. The samples collected before (PRE-Hypoxia) and after (POST-Hypoxia) the trekking in the Himalayas were compared. Satellite cells were investigated for oxidative stress (oxidant production, antioxidant enzyme activity, and lipid damage), mitochondrial potential variation, gene profile of HIF, and myogenic transcription factors (Pax7, MyoD, myogenin), and miRNA expression (miR-1, miR-133, miR-206). Results: The nuclear domain analysis showed a significant fusion and consequent reduction of the Pax7(+) satellite cells in the single mature fibers. The POST-Hypoxia myoblasts obtained by two out of six volunteers showed high superoxide anion production and lipid peroxidation along with impaired dismutase and catalase and mitochondrial potential. The transcription profile and miRNA expression were different for oxidized and non-oxidized cells. Conclusions: The present study supports the phenomenon of hypobaric-hypoxia-induced oxidative stress and its role in the impairment of the regenerative capacity of satellite cells derived from the V. Lateralis muscle of young adult female subjects. Frontiers Media S.A. 2016-07-14 /pmc/articles/PMC4943944/ /pubmed/27471475 http://dx.doi.org/10.3389/fphys.2016.00303 Text en Copyright © 2016 Mancinelli, Di Filippo, Verratti, Fulle, Toniolo, Reggiani and Pietrangelo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Mancinelli, Rosa
Di Filippo, Ester S.
Verratti, Vittore
Fulle, Stefania
Toniolo, Luana
Reggiani, Carlo
Pietrangelo, Tiziana
The Regenerative Potential of Female Skeletal Muscle upon Hypobaric Hypoxic Exposure
title The Regenerative Potential of Female Skeletal Muscle upon Hypobaric Hypoxic Exposure
title_full The Regenerative Potential of Female Skeletal Muscle upon Hypobaric Hypoxic Exposure
title_fullStr The Regenerative Potential of Female Skeletal Muscle upon Hypobaric Hypoxic Exposure
title_full_unstemmed The Regenerative Potential of Female Skeletal Muscle upon Hypobaric Hypoxic Exposure
title_short The Regenerative Potential of Female Skeletal Muscle upon Hypobaric Hypoxic Exposure
title_sort regenerative potential of female skeletal muscle upon hypobaric hypoxic exposure
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4943944/
https://www.ncbi.nlm.nih.gov/pubmed/27471475
http://dx.doi.org/10.3389/fphys.2016.00303
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