Cargando…
PIN2-like proteins may contribute to the regulation of morphogenetic processes during spermatogenesis in Chara vulgaris
KEY MESSAGE: PIN2-like auxin transporters are expressed, preferentially in a polarized manner, in antheridial cells of freshwater green algaChara vulgaris, considered to be the closest relative of the present-day land plants. ABSTRACT: Chara vulgaris represents a group of advanced multicellular gree...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4943976/ https://www.ncbi.nlm.nih.gov/pubmed/27068826 http://dx.doi.org/10.1007/s00299-016-1979-x |
_version_ | 1782442682271924224 |
---|---|
author | Żabka, Aneta Polit, Justyna Teresa Winnicki, Konrad Paciorek, Patrycja Juszczak, Jolanta Nowak, Mateusz Maszewski, Janusz |
author_facet | Żabka, Aneta Polit, Justyna Teresa Winnicki, Konrad Paciorek, Patrycja Juszczak, Jolanta Nowak, Mateusz Maszewski, Janusz |
author_sort | Żabka, Aneta |
collection | PubMed |
description | KEY MESSAGE: PIN2-like auxin transporters are expressed, preferentially in a polarized manner, in antheridial cells of freshwater green algaChara vulgaris, considered to be the closest relative of the present-day land plants. ABSTRACT: Chara vulgaris represents a group of advanced multicellular green algae that are considered as the closest relatives of the present-day land plants. A highly specialized structure of its male sex organs (antheridia) includes filaments consisting of generative cells, which after a series of synchronous divisions transform into mature sperm, and non-generative cells comprising outer shield cells, cylindrical manubria, and central complex of capitular cells from which antheridial filaments arise. Immunofluorescence observations indicate that PIN2-like proteins (PIN2-LPs), recognized by antibodies against PIN-FORMED2 (PIN2) auxin transporter in Arabidopsis thaliana, are expressed in both types of antheridial cells and, in most of them, preferentially accumulate in a polarized manner. The appearance of PIN2-LPs in germ-line cells is strictly confined to the proliferative period of spermatogenesis and their quantities increase steadily till antheridial filaments reach the 16-celled stage. An enhanced level of PIN2-LPs observed in the central cell walls separating two asynchronously developing parts of antheridial filaments (characterized by the plugged plasmodesmata) is correlated with an enhanced deposition of callose. Intense PIN2-LPs immunofluorescence maintained in the capitular cells and its altering polarity in manubria suggest a pivotal role of these cells in the regulation of auxin transport directionality during the whole time of antheridial ontogenesis. Immunohistochemical staining of IAA revealed a clear-cut correspondence between localization sites of auxins and PIN2-LPs. It seems probable then that a supplementary developmental mechanism has evolved in Chara, by which all antheridial elements may be integrated at the supra-cellular level via plasma membrane-targeted PIN2-LPs and auxin-mediated processes. |
format | Online Article Text |
id | pubmed-4943976 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-49439762016-07-26 PIN2-like proteins may contribute to the regulation of morphogenetic processes during spermatogenesis in Chara vulgaris Żabka, Aneta Polit, Justyna Teresa Winnicki, Konrad Paciorek, Patrycja Juszczak, Jolanta Nowak, Mateusz Maszewski, Janusz Plant Cell Rep Original Article KEY MESSAGE: PIN2-like auxin transporters are expressed, preferentially in a polarized manner, in antheridial cells of freshwater green algaChara vulgaris, considered to be the closest relative of the present-day land plants. ABSTRACT: Chara vulgaris represents a group of advanced multicellular green algae that are considered as the closest relatives of the present-day land plants. A highly specialized structure of its male sex organs (antheridia) includes filaments consisting of generative cells, which after a series of synchronous divisions transform into mature sperm, and non-generative cells comprising outer shield cells, cylindrical manubria, and central complex of capitular cells from which antheridial filaments arise. Immunofluorescence observations indicate that PIN2-like proteins (PIN2-LPs), recognized by antibodies against PIN-FORMED2 (PIN2) auxin transporter in Arabidopsis thaliana, are expressed in both types of antheridial cells and, in most of them, preferentially accumulate in a polarized manner. The appearance of PIN2-LPs in germ-line cells is strictly confined to the proliferative period of spermatogenesis and their quantities increase steadily till antheridial filaments reach the 16-celled stage. An enhanced level of PIN2-LPs observed in the central cell walls separating two asynchronously developing parts of antheridial filaments (characterized by the plugged plasmodesmata) is correlated with an enhanced deposition of callose. Intense PIN2-LPs immunofluorescence maintained in the capitular cells and its altering polarity in manubria suggest a pivotal role of these cells in the regulation of auxin transport directionality during the whole time of antheridial ontogenesis. Immunohistochemical staining of IAA revealed a clear-cut correspondence between localization sites of auxins and PIN2-LPs. It seems probable then that a supplementary developmental mechanism has evolved in Chara, by which all antheridial elements may be integrated at the supra-cellular level via plasma membrane-targeted PIN2-LPs and auxin-mediated processes. Springer Berlin Heidelberg 2016-04-11 2016 /pmc/articles/PMC4943976/ /pubmed/27068826 http://dx.doi.org/10.1007/s00299-016-1979-x Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Żabka, Aneta Polit, Justyna Teresa Winnicki, Konrad Paciorek, Patrycja Juszczak, Jolanta Nowak, Mateusz Maszewski, Janusz PIN2-like proteins may contribute to the regulation of morphogenetic processes during spermatogenesis in Chara vulgaris |
title | PIN2-like proteins may contribute to the regulation of morphogenetic processes during spermatogenesis in Chara vulgaris |
title_full | PIN2-like proteins may contribute to the regulation of morphogenetic processes during spermatogenesis in Chara vulgaris |
title_fullStr | PIN2-like proteins may contribute to the regulation of morphogenetic processes during spermatogenesis in Chara vulgaris |
title_full_unstemmed | PIN2-like proteins may contribute to the regulation of morphogenetic processes during spermatogenesis in Chara vulgaris |
title_short | PIN2-like proteins may contribute to the regulation of morphogenetic processes during spermatogenesis in Chara vulgaris |
title_sort | pin2-like proteins may contribute to the regulation of morphogenetic processes during spermatogenesis in chara vulgaris |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4943976/ https://www.ncbi.nlm.nih.gov/pubmed/27068826 http://dx.doi.org/10.1007/s00299-016-1979-x |
work_keys_str_mv | AT zabkaaneta pin2likeproteinsmaycontributetotheregulationofmorphogeneticprocessesduringspermatogenesisincharavulgaris AT politjustynateresa pin2likeproteinsmaycontributetotheregulationofmorphogeneticprocessesduringspermatogenesisincharavulgaris AT winnickikonrad pin2likeproteinsmaycontributetotheregulationofmorphogeneticprocessesduringspermatogenesisincharavulgaris AT paciorekpatrycja pin2likeproteinsmaycontributetotheregulationofmorphogeneticprocessesduringspermatogenesisincharavulgaris AT juszczakjolanta pin2likeproteinsmaycontributetotheregulationofmorphogeneticprocessesduringspermatogenesisincharavulgaris AT nowakmateusz pin2likeproteinsmaycontributetotheregulationofmorphogeneticprocessesduringspermatogenesisincharavulgaris AT maszewskijanusz pin2likeproteinsmaycontributetotheregulationofmorphogeneticprocessesduringspermatogenesisincharavulgaris |