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Methodology for Quantitative Characterization of Fluorophore Photoswitching to Predict Superresolution Microscopy Image Quality

Single-molecule localization microscopy (SMLM) image quality and resolution strongly depend on the photoswitching properties of fluorophores used for sample labeling. Development of fluorophores with optimized photoswitching will considerably improve SMLM spatial and spectral resolution. Currently,...

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Detalles Bibliográficos
Autores principales:	Bittel, Amy M., Nickerson, Andrew, Saldivar, Isaac S., Dolman, Nick J., Nan, Xiaolin, Gibbs, Summer L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944197/ https://www.ncbi.nlm.nih.gov/pubmed/27412307 http://dx.doi.org/10.1038/srep29687

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