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Molecular characterization of nucleoprotein gene of rabies virus from Maharashtra, India

CONTEXT: Rabies poses a serious public health concern in developing countries such as India. Aims: The study focuses on molecular diagnosis of street rabies virus (RABV) from human clinical specimens received from Maharashtra, India. MATERIALS AND METHODS: Nucleoprotein gene from eight (of total 20...

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Autores principales: Mehta, S, Charan, P, Dahake, R, Mukherjee, S, Chowdhary, A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944340/
https://www.ncbi.nlm.nih.gov/pubmed/26821566
http://dx.doi.org/10.4103/0022-3859.175006
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author Mehta, S
Charan, P
Dahake, R
Mukherjee, S
Chowdhary, A
author_facet Mehta, S
Charan, P
Dahake, R
Mukherjee, S
Chowdhary, A
author_sort Mehta, S
collection PubMed
description CONTEXT: Rabies poses a serious public health concern in developing countries such as India. Aims: The study focuses on molecular diagnosis of street rabies virus (RABV) from human clinical specimens received from Maharashtra, India. MATERIALS AND METHODS: Nucleoprotein gene from eight (of total 20 suspected samples) rabies cases that tested positive for rabies antigen using reverse transcriptase-polymerase chain reaction (RT-PCR) were sequenced. RESULTS: Sequence analysis using basic local alignment search tool (BLAST) and multiple sequence alignment (MSA) and phylogenetic analysis showed similarity to previously reported sequences from India and those of Arctic lineages. CONCLUSIONS: The circulating RABV strains in Maharashtra, India show genetic relatedness to RABV strains reported from Indo-Arctic lineages and India-South and Japan.
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spelling pubmed-49443402016-07-25 Molecular characterization of nucleoprotein gene of rabies virus from Maharashtra, India Mehta, S Charan, P Dahake, R Mukherjee, S Chowdhary, A J Postgrad Med Brief Report CONTEXT: Rabies poses a serious public health concern in developing countries such as India. Aims: The study focuses on molecular diagnosis of street rabies virus (RABV) from human clinical specimens received from Maharashtra, India. MATERIALS AND METHODS: Nucleoprotein gene from eight (of total 20 suspected samples) rabies cases that tested positive for rabies antigen using reverse transcriptase-polymerase chain reaction (RT-PCR) were sequenced. RESULTS: Sequence analysis using basic local alignment search tool (BLAST) and multiple sequence alignment (MSA) and phylogenetic analysis showed similarity to previously reported sequences from India and those of Arctic lineages. CONCLUSIONS: The circulating RABV strains in Maharashtra, India show genetic relatedness to RABV strains reported from Indo-Arctic lineages and India-South and Japan. Medknow Publications & Media Pvt Ltd 2016 /pmc/articles/PMC4944340/ /pubmed/26821566 http://dx.doi.org/10.4103/0022-3859.175006 Text en Copyright: © 2016 Journal of Postgraduate Medicine http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Brief Report
Mehta, S
Charan, P
Dahake, R
Mukherjee, S
Chowdhary, A
Molecular characterization of nucleoprotein gene of rabies virus from Maharashtra, India
title Molecular characterization of nucleoprotein gene of rabies virus from Maharashtra, India
title_full Molecular characterization of nucleoprotein gene of rabies virus from Maharashtra, India
title_fullStr Molecular characterization of nucleoprotein gene of rabies virus from Maharashtra, India
title_full_unstemmed Molecular characterization of nucleoprotein gene of rabies virus from Maharashtra, India
title_short Molecular characterization of nucleoprotein gene of rabies virus from Maharashtra, India
title_sort molecular characterization of nucleoprotein gene of rabies virus from maharashtra, india
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944340/
https://www.ncbi.nlm.nih.gov/pubmed/26821566
http://dx.doi.org/10.4103/0022-3859.175006
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