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The effect of particle size and amount of inoculum on fungal treatment of wheat straw and wood chips

BACKGROUND: The aim of this study was to optimize the fungal treatment of lignocellulosic biomass by stimulating the colonization. Wheat straw and wood chips were treated with Ceriporiopsis subvermispora and Lentinula edodes with various amounts of colonized millet grains (0.5, 1.5 or 3.0 % per g of...

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Autores principales: van Kuijk, Sandra J. A., Sonnenberg, Anton S. M., Baars, Johan J. P., Hendriks, Wouter H., Cone, John W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944425/
https://www.ncbi.nlm.nih.gov/pubmed/27418962
http://dx.doi.org/10.1186/s40104-016-0098-4
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author van Kuijk, Sandra J. A.
Sonnenberg, Anton S. M.
Baars, Johan J. P.
Hendriks, Wouter H.
Cone, John W.
author_facet van Kuijk, Sandra J. A.
Sonnenberg, Anton S. M.
Baars, Johan J. P.
Hendriks, Wouter H.
Cone, John W.
author_sort van Kuijk, Sandra J. A.
collection PubMed
description BACKGROUND: The aim of this study was to optimize the fungal treatment of lignocellulosic biomass by stimulating the colonization. Wheat straw and wood chips were treated with Ceriporiopsis subvermispora and Lentinula edodes with various amounts of colonized millet grains (0.5, 1.5 or 3.0 % per g of wet weight of substrate) added to the substrates. Also, wheat straw and wood chips were chopped to either 0.5 or 2 cm. Effectiveness of the fungal treatment after 0, 2, 4, 6, or 8 wk of incubation was determined by changes in chemical composition, in vitro gas production (IVGP) as a measure for rumen degradability, and ergosterol content as a measure of fungal biomass. RESULTS: Incomplete colonization was observed for C. subvermispora treated wheat straw and L. edodes treated wood chips. The different particle sizes and amounts of inoculum tested, had no significant effects on the chemical composition and the IVGP of C. subvermispora treated wood chips. Particle size did influence L. edodes treatment of wheat straw. The L. edodes treatment of 2 cm wheat straw resulted in a more selective delignification and a higher IVGP than the smaller particles. Addition of 1.5 % or 3 % L. edodes inoculum to wheat straw resulted in more selective delignification and a higher IVGP than addition of 0.5 % inoculum. CONCLUSION: Particle size and amount of inoculum did not have an effect on C. subvermispora treatment of wood chips. At least 1.5 % L. edodes colonized millet grains should be added to 2 cm wheat straw to result in an increased IVGP and acid detergent lignin (ADL) degradation.
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spelling pubmed-49444252016-07-15 The effect of particle size and amount of inoculum on fungal treatment of wheat straw and wood chips van Kuijk, Sandra J. A. Sonnenberg, Anton S. M. Baars, Johan J. P. Hendriks, Wouter H. Cone, John W. J Anim Sci Biotechnol Research BACKGROUND: The aim of this study was to optimize the fungal treatment of lignocellulosic biomass by stimulating the colonization. Wheat straw and wood chips were treated with Ceriporiopsis subvermispora and Lentinula edodes with various amounts of colonized millet grains (0.5, 1.5 or 3.0 % per g of wet weight of substrate) added to the substrates. Also, wheat straw and wood chips were chopped to either 0.5 or 2 cm. Effectiveness of the fungal treatment after 0, 2, 4, 6, or 8 wk of incubation was determined by changes in chemical composition, in vitro gas production (IVGP) as a measure for rumen degradability, and ergosterol content as a measure of fungal biomass. RESULTS: Incomplete colonization was observed for C. subvermispora treated wheat straw and L. edodes treated wood chips. The different particle sizes and amounts of inoculum tested, had no significant effects on the chemical composition and the IVGP of C. subvermispora treated wood chips. Particle size did influence L. edodes treatment of wheat straw. The L. edodes treatment of 2 cm wheat straw resulted in a more selective delignification and a higher IVGP than the smaller particles. Addition of 1.5 % or 3 % L. edodes inoculum to wheat straw resulted in more selective delignification and a higher IVGP than addition of 0.5 % inoculum. CONCLUSION: Particle size and amount of inoculum did not have an effect on C. subvermispora treatment of wood chips. At least 1.5 % L. edodes colonized millet grains should be added to 2 cm wheat straw to result in an increased IVGP and acid detergent lignin (ADL) degradation. BioMed Central 2016-07-13 /pmc/articles/PMC4944425/ /pubmed/27418962 http://dx.doi.org/10.1186/s40104-016-0098-4 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
van Kuijk, Sandra J. A.
Sonnenberg, Anton S. M.
Baars, Johan J. P.
Hendriks, Wouter H.
Cone, John W.
The effect of particle size and amount of inoculum on fungal treatment of wheat straw and wood chips
title The effect of particle size and amount of inoculum on fungal treatment of wheat straw and wood chips
title_full The effect of particle size and amount of inoculum on fungal treatment of wheat straw and wood chips
title_fullStr The effect of particle size and amount of inoculum on fungal treatment of wheat straw and wood chips
title_full_unstemmed The effect of particle size and amount of inoculum on fungal treatment of wheat straw and wood chips
title_short The effect of particle size and amount of inoculum on fungal treatment of wheat straw and wood chips
title_sort effect of particle size and amount of inoculum on fungal treatment of wheat straw and wood chips
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944425/
https://www.ncbi.nlm.nih.gov/pubmed/27418962
http://dx.doi.org/10.1186/s40104-016-0098-4
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