Immobilized WNT Proteins Act as a Stem Cell Niche for Tissue Engineering

The timing, location, and level of WNT signaling are highly regulated during embryonic development and for the maintenance of adult tissues. Consequently the ability to provide a defined and directed source of WNT proteins is crucial to fully understand its role in tissue development and to mimic it...

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Detalles Bibliográficos
Autores principales: Lowndes, Molly, Rotherham, Michael, Price, Joshua C., El Haj, Alicia J., Habib, Shukry J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Resource
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944585/
https://www.ncbi.nlm.nih.gov/pubmed/27411105
http://dx.doi.org/10.1016/j.stemcr.2016.06.004
Descripción
Sumario:The timing, location, and level of WNT signaling are highly regulated during embryonic development and for the maintenance of adult tissues. Consequently the ability to provide a defined and directed source of WNT proteins is crucial to fully understand its role in tissue development and to mimic its activity in vitro. Here we describe a one-step immobilization technique to covalently bind WNT3A proteins as a basal surface with easy storage and long-lasting activity. We show that this platform is able to maintain adult and embryonic stem cells while also being adaptable for 3D systems. Therefore, this platform could be used for recapitulating specific stem cell niches with the goal of improving tissue engineering.

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