Immobilized WNT Proteins Act as a Stem Cell Niche for Tissue Engineering

The timing, location, and level of WNT signaling are highly regulated during embryonic development and for the maintenance of adult tissues. Consequently the ability to provide a defined and directed source of WNT proteins is crucial to fully understand its role in tissue development and to mimic it...

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Detalles Bibliográficos
Autores principales: Lowndes, Molly, Rotherham, Michael, Price, Joshua C., El Haj, Alicia J., Habib, Shukry J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Resource
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944585/
https://www.ncbi.nlm.nih.gov/pubmed/27411105
http://dx.doi.org/10.1016/j.stemcr.2016.06.004
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author Lowndes, Molly
Rotherham, Michael
Price, Joshua C.
El Haj, Alicia J.
Habib, Shukry J.
author_facet Lowndes, Molly
Rotherham, Michael
Price, Joshua C.
El Haj, Alicia J.
Habib, Shukry J.
author_sort Lowndes, Molly
collection PubMed
description The timing, location, and level of WNT signaling are highly regulated during embryonic development and for the maintenance of adult tissues. Consequently the ability to provide a defined and directed source of WNT proteins is crucial to fully understand its role in tissue development and to mimic its activity in vitro. Here we describe a one-step immobilization technique to covalently bind WNT3A proteins as a basal surface with easy storage and long-lasting activity. We show that this platform is able to maintain adult and embryonic stem cells while also being adaptable for 3D systems. Therefore, this platform could be used for recapitulating specific stem cell niches with the goal of improving tissue engineering.
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spelling pubmed-49445852016-07-22 Immobilized WNT Proteins Act as a Stem Cell Niche for Tissue Engineering Lowndes, Molly Rotherham, Michael Price, Joshua C. El Haj, Alicia J. Habib, Shukry J. Stem Cell Reports Resource The timing, location, and level of WNT signaling are highly regulated during embryonic development and for the maintenance of adult tissues. Consequently the ability to provide a defined and directed source of WNT proteins is crucial to fully understand its role in tissue development and to mimic its activity in vitro. Here we describe a one-step immobilization technique to covalently bind WNT3A proteins as a basal surface with easy storage and long-lasting activity. We show that this platform is able to maintain adult and embryonic stem cells while also being adaptable for 3D systems. Therefore, this platform could be used for recapitulating specific stem cell niches with the goal of improving tissue engineering. Elsevier 2016-07-12 /pmc/articles/PMC4944585/ /pubmed/27411105 http://dx.doi.org/10.1016/j.stemcr.2016.06.004 Text en © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Resource
Lowndes, Molly
Rotherham, Michael
Price, Joshua C.
El Haj, Alicia J.
Habib, Shukry J.
Immobilized WNT Proteins Act as a Stem Cell Niche for Tissue Engineering
title Immobilized WNT Proteins Act as a Stem Cell Niche for Tissue Engineering
title_full Immobilized WNT Proteins Act as a Stem Cell Niche for Tissue Engineering
title_fullStr Immobilized WNT Proteins Act as a Stem Cell Niche for Tissue Engineering
title_full_unstemmed Immobilized WNT Proteins Act as a Stem Cell Niche for Tissue Engineering
title_short Immobilized WNT Proteins Act as a Stem Cell Niche for Tissue Engineering
title_sort immobilized wnt proteins act as a stem cell niche for tissue engineering
topic Resource
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944585/
https://www.ncbi.nlm.nih.gov/pubmed/27411105
http://dx.doi.org/10.1016/j.stemcr.2016.06.004
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AT elhajaliciaj immobilizedwntproteinsactasastemcellnichefortissueengineering
AT habibshukryj immobilizedwntproteinsactasastemcellnichefortissueengineering

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