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The Calponin Family Member CHDP-1 Interacts with Rac/CED-10 to Promote Cell Protrusions
Eukaryotic cells extend a variety of surface protrusions to direct cell motility. Formation of protrusions is mediated by coordinated actions between the plasma membrane and the underlying actin cytoskeleton. Here, we found that the single calponin homology (CH) domain-containing protein CHDP-1 indu...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944944/ https://www.ncbi.nlm.nih.gov/pubmed/27415421 http://dx.doi.org/10.1371/journal.pgen.1006163 |
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author | Guan, Liying Ma, Xuehua Zhang, Jingyan Liu, Jia-Jia Wang, Yingchun Ding, Mei |
author_facet | Guan, Liying Ma, Xuehua Zhang, Jingyan Liu, Jia-Jia Wang, Yingchun Ding, Mei |
author_sort | Guan, Liying |
collection | PubMed |
description | Eukaryotic cells extend a variety of surface protrusions to direct cell motility. Formation of protrusions is mediated by coordinated actions between the plasma membrane and the underlying actin cytoskeleton. Here, we found that the single calponin homology (CH) domain-containing protein CHDP-1 induces the formation of cell protrusions in C. elegans. CHDP-1 is anchored to the cortex through its amphipathic helix. CHDP-1 associates through its CH domain with the small GTPase Rac1/CED-10, which is a key regulator of the actin cytoskeleton. CHDP-1 preferentially binds to the GTP-bound active form of the CED-10 protein and preserves the membrane localization of GTP-CED-10. Hence, by coupling membrane expansion to Rac1-mediated actin dynamics, CHDP-1 promotes the formation of cellular protrusions in vivo. |
format | Online Article Text |
id | pubmed-4944944 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-49449442016-08-08 The Calponin Family Member CHDP-1 Interacts with Rac/CED-10 to Promote Cell Protrusions Guan, Liying Ma, Xuehua Zhang, Jingyan Liu, Jia-Jia Wang, Yingchun Ding, Mei PLoS Genet Research Article Eukaryotic cells extend a variety of surface protrusions to direct cell motility. Formation of protrusions is mediated by coordinated actions between the plasma membrane and the underlying actin cytoskeleton. Here, we found that the single calponin homology (CH) domain-containing protein CHDP-1 induces the formation of cell protrusions in C. elegans. CHDP-1 is anchored to the cortex through its amphipathic helix. CHDP-1 associates through its CH domain with the small GTPase Rac1/CED-10, which is a key regulator of the actin cytoskeleton. CHDP-1 preferentially binds to the GTP-bound active form of the CED-10 protein and preserves the membrane localization of GTP-CED-10. Hence, by coupling membrane expansion to Rac1-mediated actin dynamics, CHDP-1 promotes the formation of cellular protrusions in vivo. Public Library of Science 2016-07-14 /pmc/articles/PMC4944944/ /pubmed/27415421 http://dx.doi.org/10.1371/journal.pgen.1006163 Text en © 2016 Guan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Guan, Liying Ma, Xuehua Zhang, Jingyan Liu, Jia-Jia Wang, Yingchun Ding, Mei The Calponin Family Member CHDP-1 Interacts with Rac/CED-10 to Promote Cell Protrusions |
title | The Calponin Family Member CHDP-1 Interacts with Rac/CED-10 to Promote Cell Protrusions |
title_full | The Calponin Family Member CHDP-1 Interacts with Rac/CED-10 to Promote Cell Protrusions |
title_fullStr | The Calponin Family Member CHDP-1 Interacts with Rac/CED-10 to Promote Cell Protrusions |
title_full_unstemmed | The Calponin Family Member CHDP-1 Interacts with Rac/CED-10 to Promote Cell Protrusions |
title_short | The Calponin Family Member CHDP-1 Interacts with Rac/CED-10 to Promote Cell Protrusions |
title_sort | calponin family member chdp-1 interacts with rac/ced-10 to promote cell protrusions |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944944/ https://www.ncbi.nlm.nih.gov/pubmed/27415421 http://dx.doi.org/10.1371/journal.pgen.1006163 |
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