Sprouty2 Suppresses Epithelial-Mesenchymal Transition of Human Lens Epithelial Cells through Blockade of Smad2 and ERK1/2 Pathways

Transforming growth factor β (TGFβ)-induced epithelial-mesenchymal transition (EMT) of lens epithelial cells (LECs) plays a key role in the pathogenesis of anterior subcapsular cataract (ASC) and capsule opacification. In mouse lens, Sprouty2 (Spry2) has a negative regulatory role on TGFβ signaling....

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Autores principales: Tan, Xuhua, Zhu, Yi, Chen, Chuan, Chen, Xiaoyun, Qin, Yingyan, Qu, Bo, Luo, Lixia, Lin, Haotian, Wu, Mingxing, Chen, Weirong, Liu, Yizhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944964/
https://www.ncbi.nlm.nih.gov/pubmed/27415760
http://dx.doi.org/10.1371/journal.pone.0159275
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author Tan, Xuhua
Zhu, Yi
Chen, Chuan
Chen, Xiaoyun
Qin, Yingyan
Qu, Bo
Luo, Lixia
Lin, Haotian
Wu, Mingxing
Chen, Weirong
Liu, Yizhi
author_facet Tan, Xuhua
Zhu, Yi
Chen, Chuan
Chen, Xiaoyun
Qin, Yingyan
Qu, Bo
Luo, Lixia
Lin, Haotian
Wu, Mingxing
Chen, Weirong
Liu, Yizhi
author_sort Tan, Xuhua
collection PubMed
description Transforming growth factor β (TGFβ)-induced epithelial-mesenchymal transition (EMT) of lens epithelial cells (LECs) plays a key role in the pathogenesis of anterior subcapsular cataract (ASC) and capsule opacification. In mouse lens, Sprouty2 (Spry2) has a negative regulatory role on TGFβ signaling. However, the regulation of Spry2 during ASC development and how Spry2 modulates TGFβ signaling pathway in human LECs have not been characterized. Here, we demonstrate that Spry2 expression level is decreased in anterior capsule LECs of ASC patients. Spry2 negatively regulates TGFβ2-induced EMT and migration of LECs through inhibition of Smad2 and ERK1/2 phosphorylation. Also, blockade of Smad2 or ERK1/2 activation suppresses EMT caused by Spry2 downregulation. Collectively, our results for the first time show in human LECs that Spry2 has an inhibitory role in TGFβ signaling pathway. Our findings in human lens tissue and epithelial cells suggest that Spry2 may become a novel therapeutic target for the prevention and treatment of ASC and capsule opacification.
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spelling pubmed-49449642016-08-08 Sprouty2 Suppresses Epithelial-Mesenchymal Transition of Human Lens Epithelial Cells through Blockade of Smad2 and ERK1/2 Pathways Tan, Xuhua Zhu, Yi Chen, Chuan Chen, Xiaoyun Qin, Yingyan Qu, Bo Luo, Lixia Lin, Haotian Wu, Mingxing Chen, Weirong Liu, Yizhi PLoS One Research Article Transforming growth factor β (TGFβ)-induced epithelial-mesenchymal transition (EMT) of lens epithelial cells (LECs) plays a key role in the pathogenesis of anterior subcapsular cataract (ASC) and capsule opacification. In mouse lens, Sprouty2 (Spry2) has a negative regulatory role on TGFβ signaling. However, the regulation of Spry2 during ASC development and how Spry2 modulates TGFβ signaling pathway in human LECs have not been characterized. Here, we demonstrate that Spry2 expression level is decreased in anterior capsule LECs of ASC patients. Spry2 negatively regulates TGFβ2-induced EMT and migration of LECs through inhibition of Smad2 and ERK1/2 phosphorylation. Also, blockade of Smad2 or ERK1/2 activation suppresses EMT caused by Spry2 downregulation. Collectively, our results for the first time show in human LECs that Spry2 has an inhibitory role in TGFβ signaling pathway. Our findings in human lens tissue and epithelial cells suggest that Spry2 may become a novel therapeutic target for the prevention and treatment of ASC and capsule opacification. Public Library of Science 2016-07-14 /pmc/articles/PMC4944964/ /pubmed/27415760 http://dx.doi.org/10.1371/journal.pone.0159275 Text en © 2016 Tan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Tan, Xuhua
Zhu, Yi
Chen, Chuan
Chen, Xiaoyun
Qin, Yingyan
Qu, Bo
Luo, Lixia
Lin, Haotian
Wu, Mingxing
Chen, Weirong
Liu, Yizhi
Sprouty2 Suppresses Epithelial-Mesenchymal Transition of Human Lens Epithelial Cells through Blockade of Smad2 and ERK1/2 Pathways
title Sprouty2 Suppresses Epithelial-Mesenchymal Transition of Human Lens Epithelial Cells through Blockade of Smad2 and ERK1/2 Pathways
title_full Sprouty2 Suppresses Epithelial-Mesenchymal Transition of Human Lens Epithelial Cells through Blockade of Smad2 and ERK1/2 Pathways
title_fullStr Sprouty2 Suppresses Epithelial-Mesenchymal Transition of Human Lens Epithelial Cells through Blockade of Smad2 and ERK1/2 Pathways
title_full_unstemmed Sprouty2 Suppresses Epithelial-Mesenchymal Transition of Human Lens Epithelial Cells through Blockade of Smad2 and ERK1/2 Pathways
title_short Sprouty2 Suppresses Epithelial-Mesenchymal Transition of Human Lens Epithelial Cells through Blockade of Smad2 and ERK1/2 Pathways
title_sort sprouty2 suppresses epithelial-mesenchymal transition of human lens epithelial cells through blockade of smad2 and erk1/2 pathways
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944964/
https://www.ncbi.nlm.nih.gov/pubmed/27415760
http://dx.doi.org/10.1371/journal.pone.0159275
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