Characterization of Transgenic Silkworm Yielded Biomaterials with Calcium-Binding Activity

Silk fibers have many inherent properties that are suitable for their use in biomaterials. In this study, the silk fibroin was genetically modified by including a Ca-binding sequence, [(AGSGAG)(6)ASEYDYDDDSDDDDEWD](2) from shell nacreous matrix protein. It can be produced as fibers by transgenic sil...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Shaohua, Zhang, Yuyu, Yang, Mingying, Ye, Lupeng, Gong, Lu, Qian, Qiujie, Shuai, Yajun, You, Zhengying, Chen, Yuyin, Zhong, Boxiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944971/
https://www.ncbi.nlm.nih.gov/pubmed/27414647
http://dx.doi.org/10.1371/journal.pone.0159111
_version_ 1782442843076296704
author Wang, Shaohua
Zhang, Yuyu
Yang, Mingying
Ye, Lupeng
Gong, Lu
Qian, Qiujie
Shuai, Yajun
You, Zhengying
Chen, Yuyin
Zhong, Boxiong
author_facet Wang, Shaohua
Zhang, Yuyu
Yang, Mingying
Ye, Lupeng
Gong, Lu
Qian, Qiujie
Shuai, Yajun
You, Zhengying
Chen, Yuyin
Zhong, Boxiong
author_sort Wang, Shaohua
collection PubMed
description Silk fibers have many inherent properties that are suitable for their use in biomaterials. In this study, the silk fibroin was genetically modified by including a Ca-binding sequence, [(AGSGAG)(6)ASEYDYDDDSDDDDEWD](2) from shell nacreous matrix protein. It can be produced as fibers by transgenic silkworm. The Ca-binding activity and mineralization of the transgenic silk fibroin were examined in vitro. The results showed that this transgenic silk fibroin had relatively higher Ca-binding activity than unmodified silk fibroin. The increased Ca-binding activity could promote the usage of silk fibroin as a biomaterial in the pharmaceutical industry. This study shows the possibility of using silk fibroin as a mineralization accelerating medical material by generating genetically modified transgenic silkworm.
format Online
Article
Text
id pubmed-4944971
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-49449712016-08-08 Characterization of Transgenic Silkworm Yielded Biomaterials with Calcium-Binding Activity Wang, Shaohua Zhang, Yuyu Yang, Mingying Ye, Lupeng Gong, Lu Qian, Qiujie Shuai, Yajun You, Zhengying Chen, Yuyin Zhong, Boxiong PLoS One Research Article Silk fibers have many inherent properties that are suitable for their use in biomaterials. In this study, the silk fibroin was genetically modified by including a Ca-binding sequence, [(AGSGAG)(6)ASEYDYDDDSDDDDEWD](2) from shell nacreous matrix protein. It can be produced as fibers by transgenic silkworm. The Ca-binding activity and mineralization of the transgenic silk fibroin were examined in vitro. The results showed that this transgenic silk fibroin had relatively higher Ca-binding activity than unmodified silk fibroin. The increased Ca-binding activity could promote the usage of silk fibroin as a biomaterial in the pharmaceutical industry. This study shows the possibility of using silk fibroin as a mineralization accelerating medical material by generating genetically modified transgenic silkworm. Public Library of Science 2016-07-14 /pmc/articles/PMC4944971/ /pubmed/27414647 http://dx.doi.org/10.1371/journal.pone.0159111 Text en © 2016 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wang, Shaohua
Zhang, Yuyu
Yang, Mingying
Ye, Lupeng
Gong, Lu
Qian, Qiujie
Shuai, Yajun
You, Zhengying
Chen, Yuyin
Zhong, Boxiong
Characterization of Transgenic Silkworm Yielded Biomaterials with Calcium-Binding Activity
title Characterization of Transgenic Silkworm Yielded Biomaterials with Calcium-Binding Activity
title_full Characterization of Transgenic Silkworm Yielded Biomaterials with Calcium-Binding Activity
title_fullStr Characterization of Transgenic Silkworm Yielded Biomaterials with Calcium-Binding Activity
title_full_unstemmed Characterization of Transgenic Silkworm Yielded Biomaterials with Calcium-Binding Activity
title_short Characterization of Transgenic Silkworm Yielded Biomaterials with Calcium-Binding Activity
title_sort characterization of transgenic silkworm yielded biomaterials with calcium-binding activity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944971/
https://www.ncbi.nlm.nih.gov/pubmed/27414647
http://dx.doi.org/10.1371/journal.pone.0159111
work_keys_str_mv AT wangshaohua characterizationoftransgenicsilkwormyieldedbiomaterialswithcalciumbindingactivity
AT zhangyuyu characterizationoftransgenicsilkwormyieldedbiomaterialswithcalciumbindingactivity
AT yangmingying characterizationoftransgenicsilkwormyieldedbiomaterialswithcalciumbindingactivity
AT yelupeng characterizationoftransgenicsilkwormyieldedbiomaterialswithcalciumbindingactivity
AT gonglu characterizationoftransgenicsilkwormyieldedbiomaterialswithcalciumbindingactivity
AT qianqiujie characterizationoftransgenicsilkwormyieldedbiomaterialswithcalciumbindingactivity
AT shuaiyajun characterizationoftransgenicsilkwormyieldedbiomaterialswithcalciumbindingactivity
AT youzhengying characterizationoftransgenicsilkwormyieldedbiomaterialswithcalciumbindingactivity
AT chenyuyin characterizationoftransgenicsilkwormyieldedbiomaterialswithcalciumbindingactivity
AT zhongboxiong characterizationoftransgenicsilkwormyieldedbiomaterialswithcalciumbindingactivity

Ejemplares similares