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Specific requirements of nonbilayer phospholipids in mitochondrial respiratory chain function and formation

Mitochondrial membrane phospholipid composition affects mitochondrial function by influencing the assembly of the mitochondrial respiratory chain (MRC) complexes into supercomplexes. For example, the loss of cardiolipin (CL), a signature non–bilayer-forming phospholipid of mitochondria, results in d...

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Autores principales: Baker, Charli D., Basu Ball, Writoban, Pryce, Erin N., Gohil, Vishal M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4945136/
https://www.ncbi.nlm.nih.gov/pubmed/27226479
http://dx.doi.org/10.1091/mbc.E15-12-0865
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author Baker, Charli D.
Basu Ball, Writoban
Pryce, Erin N.
Gohil, Vishal M.
author_facet Baker, Charli D.
Basu Ball, Writoban
Pryce, Erin N.
Gohil, Vishal M.
author_sort Baker, Charli D.
collection PubMed
description Mitochondrial membrane phospholipid composition affects mitochondrial function by influencing the assembly of the mitochondrial respiratory chain (MRC) complexes into supercomplexes. For example, the loss of cardiolipin (CL), a signature non–bilayer-forming phospholipid of mitochondria, results in disruption of MRC supercomplexes. However, the functions of the most abundant mitochondrial phospholipids, bilayer-forming phosphatidylcholine (PC) and non–bilayer-forming phosphatidylethanolamine (PE), are not clearly defined. Using yeast mutants of PE and PC biosynthetic pathways, we show a specific requirement for mitochondrial PE in MRC complex III and IV activities but not for their formation, whereas loss of PC does not affect MRC function or formation. Unlike CL, mitochondrial PE or PC is not required for MRC supercomplex formation, emphasizing the specific requirement of CL in supercomplex assembly. Of interest, PE biosynthesized in the endoplasmic reticulum (ER) can functionally substitute for the lack of mitochondrial PE biosynthesis, suggesting the existence of PE transport pathway from ER to mitochondria. To understand the mechanism of PE transport, we disrupted ER–mitochondrial contact sites formed by the ERMES complex and found that, although not essential for PE transport, ERMES facilitates the efficient rescue of mitochondrial PE deficiency. Our work highlights specific roles of non–bilayer-forming phospholipids in MRC function and formation.
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spelling pubmed-49451362016-09-30 Specific requirements of nonbilayer phospholipids in mitochondrial respiratory chain function and formation Baker, Charli D. Basu Ball, Writoban Pryce, Erin N. Gohil, Vishal M. Mol Biol Cell Articles Mitochondrial membrane phospholipid composition affects mitochondrial function by influencing the assembly of the mitochondrial respiratory chain (MRC) complexes into supercomplexes. For example, the loss of cardiolipin (CL), a signature non–bilayer-forming phospholipid of mitochondria, results in disruption of MRC supercomplexes. However, the functions of the most abundant mitochondrial phospholipids, bilayer-forming phosphatidylcholine (PC) and non–bilayer-forming phosphatidylethanolamine (PE), are not clearly defined. Using yeast mutants of PE and PC biosynthetic pathways, we show a specific requirement for mitochondrial PE in MRC complex III and IV activities but not for their formation, whereas loss of PC does not affect MRC function or formation. Unlike CL, mitochondrial PE or PC is not required for MRC supercomplex formation, emphasizing the specific requirement of CL in supercomplex assembly. Of interest, PE biosynthesized in the endoplasmic reticulum (ER) can functionally substitute for the lack of mitochondrial PE biosynthesis, suggesting the existence of PE transport pathway from ER to mitochondria. To understand the mechanism of PE transport, we disrupted ER–mitochondrial contact sites formed by the ERMES complex and found that, although not essential for PE transport, ERMES facilitates the efficient rescue of mitochondrial PE deficiency. Our work highlights specific roles of non–bilayer-forming phospholipids in MRC function and formation. The American Society for Cell Biology 2016-07-15 /pmc/articles/PMC4945136/ /pubmed/27226479 http://dx.doi.org/10.1091/mbc.E15-12-0865 Text en © 2016 Baker et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Baker, Charli D.
Basu Ball, Writoban
Pryce, Erin N.
Gohil, Vishal M.
Specific requirements of nonbilayer phospholipids in mitochondrial respiratory chain function and formation
title Specific requirements of nonbilayer phospholipids in mitochondrial respiratory chain function and formation
title_full Specific requirements of nonbilayer phospholipids in mitochondrial respiratory chain function and formation
title_fullStr Specific requirements of nonbilayer phospholipids in mitochondrial respiratory chain function and formation
title_full_unstemmed Specific requirements of nonbilayer phospholipids in mitochondrial respiratory chain function and formation
title_short Specific requirements of nonbilayer phospholipids in mitochondrial respiratory chain function and formation
title_sort specific requirements of nonbilayer phospholipids in mitochondrial respiratory chain function and formation
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4945136/
https://www.ncbi.nlm.nih.gov/pubmed/27226479
http://dx.doi.org/10.1091/mbc.E15-12-0865
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