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Red Cell Properties after Different Modes of Blood Transportation
Transportation of blood samples is unavoidable for assessment of specific parameters in blood of patients with rare anemias, blood doping testing, or for research purposes. Despite the awareness that shipment may substantially alter multiple parameters, no study of that extent has been performed to...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4945647/ https://www.ncbi.nlm.nih.gov/pubmed/27471472 http://dx.doi.org/10.3389/fphys.2016.00288 |
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author | Makhro, Asya Huisjes, Rick Verhagen, Liesbeth P. Mañú-Pereira, María del Mar Llaudet-Planas, Esther Petkova-Kirova, Polina Wang, Jue Eichler, Hermann Bogdanova, Anna van Wijk, Richard Vives-Corrons, Joan-Lluís Kaestner, Lars |
author_facet | Makhro, Asya Huisjes, Rick Verhagen, Liesbeth P. Mañú-Pereira, María del Mar Llaudet-Planas, Esther Petkova-Kirova, Polina Wang, Jue Eichler, Hermann Bogdanova, Anna van Wijk, Richard Vives-Corrons, Joan-Lluís Kaestner, Lars |
author_sort | Makhro, Asya |
collection | PubMed |
description | Transportation of blood samples is unavoidable for assessment of specific parameters in blood of patients with rare anemias, blood doping testing, or for research purposes. Despite the awareness that shipment may substantially alter multiple parameters, no study of that extent has been performed to assess these changes and optimize shipment conditions to reduce transportation-related artifacts. Here we investigate the changes in multiple parameters in blood of healthy donors over 72 h of simulated shipment conditions. Three different anticoagulants (K3EDTA, Sodium Heparin, and citrate-based CPDA) for two temperatures (4°C and room temperature) were tested to define the optimal transportation conditions. Parameters measured cover common cytology and biochemistry parameters (complete blood count, hematocrit, morphological examination), red blood cell (RBC) volume, ion content and density, membrane properties and stability (hemolysis, osmotic fragility, membrane heat stability, patch-clamp investigations, and formation of micro vesicles), Ca(2+) handling, RBC metabolism, activity of numerous enzymes, and O(2) transport capacity. Our findings indicate that individual sets of parameters may require different shipment settings (anticoagulants, temperature). Most of the parameters except for ion (Na(+), K(+), Ca(2+)) handling and, possibly, reticulocytes counts, tend to favor transportation at 4°C. Whereas plasma and intraerythrocytic Ca(2+) cannot be accurately measured in the presence of chelators such as citrate and EDTA, the majority of Ca(2+)-dependent parameters are stabilized in CPDA samples. Even in blood samples from healthy donors transported using an optimized shipment protocol, the majority of parameters were stable within 24 h, a condition that may not hold for the samples of patients with rare anemias. This implies for as short as possible shipping using fast courier services to the closest expert laboratory at reach. Mobile laboratories or the travel of the patients to the specialized laboratories may be the only option for some groups of patients with highly unstable RBCs. |
format | Online Article Text |
id | pubmed-4945647 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-49456472016-07-28 Red Cell Properties after Different Modes of Blood Transportation Makhro, Asya Huisjes, Rick Verhagen, Liesbeth P. Mañú-Pereira, María del Mar Llaudet-Planas, Esther Petkova-Kirova, Polina Wang, Jue Eichler, Hermann Bogdanova, Anna van Wijk, Richard Vives-Corrons, Joan-Lluís Kaestner, Lars Front Physiol Physiology Transportation of blood samples is unavoidable for assessment of specific parameters in blood of patients with rare anemias, blood doping testing, or for research purposes. Despite the awareness that shipment may substantially alter multiple parameters, no study of that extent has been performed to assess these changes and optimize shipment conditions to reduce transportation-related artifacts. Here we investigate the changes in multiple parameters in blood of healthy donors over 72 h of simulated shipment conditions. Three different anticoagulants (K3EDTA, Sodium Heparin, and citrate-based CPDA) for two temperatures (4°C and room temperature) were tested to define the optimal transportation conditions. Parameters measured cover common cytology and biochemistry parameters (complete blood count, hematocrit, morphological examination), red blood cell (RBC) volume, ion content and density, membrane properties and stability (hemolysis, osmotic fragility, membrane heat stability, patch-clamp investigations, and formation of micro vesicles), Ca(2+) handling, RBC metabolism, activity of numerous enzymes, and O(2) transport capacity. Our findings indicate that individual sets of parameters may require different shipment settings (anticoagulants, temperature). Most of the parameters except for ion (Na(+), K(+), Ca(2+)) handling and, possibly, reticulocytes counts, tend to favor transportation at 4°C. Whereas plasma and intraerythrocytic Ca(2+) cannot be accurately measured in the presence of chelators such as citrate and EDTA, the majority of Ca(2+)-dependent parameters are stabilized in CPDA samples. Even in blood samples from healthy donors transported using an optimized shipment protocol, the majority of parameters were stable within 24 h, a condition that may not hold for the samples of patients with rare anemias. This implies for as short as possible shipping using fast courier services to the closest expert laboratory at reach. Mobile laboratories or the travel of the patients to the specialized laboratories may be the only option for some groups of patients with highly unstable RBCs. Frontiers Media S.A. 2016-07-15 /pmc/articles/PMC4945647/ /pubmed/27471472 http://dx.doi.org/10.3389/fphys.2016.00288 Text en Copyright © 2016 Makhro, Huisjes, Verhagen, Mañú-Pereira, Llaudet-Planas, Petkova-Kirova, Wang, Eichler, Bogdanova, van Wijk, Vives-Corrons and Kaestner. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Makhro, Asya Huisjes, Rick Verhagen, Liesbeth P. Mañú-Pereira, María del Mar Llaudet-Planas, Esther Petkova-Kirova, Polina Wang, Jue Eichler, Hermann Bogdanova, Anna van Wijk, Richard Vives-Corrons, Joan-Lluís Kaestner, Lars Red Cell Properties after Different Modes of Blood Transportation |
title | Red Cell Properties after Different Modes of Blood Transportation |
title_full | Red Cell Properties after Different Modes of Blood Transportation |
title_fullStr | Red Cell Properties after Different Modes of Blood Transportation |
title_full_unstemmed | Red Cell Properties after Different Modes of Blood Transportation |
title_short | Red Cell Properties after Different Modes of Blood Transportation |
title_sort | red cell properties after different modes of blood transportation |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4945647/ https://www.ncbi.nlm.nih.gov/pubmed/27471472 http://dx.doi.org/10.3389/fphys.2016.00288 |
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