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Association of p53 codon72 Arg>Pro polymorphism with susceptibility to nasopharyngeal carcinoma: evidence from a case–control study and meta-analysis

Tumor suppressor p53 is a critical player in the fight against cancer as it controls the cell cycle check point, apoptotic pathways and genomic stability. It is known to be the most frequently mutated gene in a wide variety of human cancers. Single-nucleotide polymorphism of p53 at codon72 leading t...

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Autores principales: Sahu, S K, Chakrabarti, S, Roy, S D, Baishya, N, Reddy, R R, Suklabaidya, S, Kumar, A, Mohanty, S, Maji, S, Suryanwanshi, A, Rajasubramaniam, S, Asthana, M, Panda, A K, Singh, S P, Ganguly, S, Shaw, O P, Bichhwalia, A K, Sahoo, P K, Chattopadhyay, N R, Chatterjee, K, Kundu, C N, Das, A K, Kannan, R, Zorenpuii, Zomawia, E, Sema, S A, Singh, Y I, Ghosh, S K, Sharma, K, Das, B S, Choudhuri, T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4945748/
https://www.ncbi.nlm.nih.gov/pubmed/27159678
http://dx.doi.org/10.1038/oncsis.2016.31
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author Sahu, S K
Chakrabarti, S
Roy, S D
Baishya, N
Reddy, R R
Suklabaidya, S
Kumar, A
Mohanty, S
Maji, S
Suryanwanshi, A
Rajasubramaniam, S
Asthana, M
Panda, A K
Singh, S P
Ganguly, S
Shaw, O P
Bichhwalia, A K
Sahoo, P K
Chattopadhyay, N R
Chatterjee, K
Kundu, C N
Das, A K
Kannan, R
Zorenpuii
Zomawia, E
Sema, S A
Singh, Y I
Ghosh, S K
Sharma, K
Das, B S
Choudhuri, T
author_facet Sahu, S K
Chakrabarti, S
Roy, S D
Baishya, N
Reddy, R R
Suklabaidya, S
Kumar, A
Mohanty, S
Maji, S
Suryanwanshi, A
Rajasubramaniam, S
Asthana, M
Panda, A K
Singh, S P
Ganguly, S
Shaw, O P
Bichhwalia, A K
Sahoo, P K
Chattopadhyay, N R
Chatterjee, K
Kundu, C N
Das, A K
Kannan, R
Zorenpuii
Zomawia, E
Sema, S A
Singh, Y I
Ghosh, S K
Sharma, K
Das, B S
Choudhuri, T
author_sort Sahu, S K
collection PubMed
description Tumor suppressor p53 is a critical player in the fight against cancer as it controls the cell cycle check point, apoptotic pathways and genomic stability. It is known to be the most frequently mutated gene in a wide variety of human cancers. Single-nucleotide polymorphism of p53 at codon72 leading to substitution of proline (Pro) in place of arginine (Arg) has been identified as a risk factor for development of many cancers, including nasopharyngeal carcinoma (NPC). However, the association of this polymorphism with NPC across the published literature has shown conflicting results. We aimed to conduct a case–control study for a possible relation of p53 codon72 Arg>Pro polymorphism with NPC risk in underdeveloped states of India, combine the result with previously available records from different databases and perform a meta-analysis to draw a more definitive conclusion. A total of 70 NPC patients and 70 healthy controls were enrolled from different hospitals of north-eastern India. The p53 codon72 Arg>Pro polymorphism was typed by polymerase chain reaction, which showed an association with NPC risk. In the meta-analysis consisting of 1842 cases and 2330 controls, it was found that individuals carrying the Pro allele and the ProPro genotype were at a significantly higher risk for NPC as compared with those with the Arg allele and the ArgArg genotype, respectively. Individuals with a ProPro genotype and a combined Pro genotype (ProPro+ArgPro) also showed a significantly higher risk for NPC over a wild homozygote ArgArg genotype. Additionally, the strength of each study was tested by power analysis and genotype distribution by Hardy–Weinberg equilibrium. The outcome of the study indicated that both allele frequency and genotype distribution of p53 codon72 Arg>Pro polymorphism were significantly associated with NPC risk. Stratified analyses based on ethnicity and source of samples supported the above result.
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spelling pubmed-49457482017-01-17 Association of p53 codon72 Arg>Pro polymorphism with susceptibility to nasopharyngeal carcinoma: evidence from a case–control study and meta-analysis Sahu, S K Chakrabarti, S Roy, S D Baishya, N Reddy, R R Suklabaidya, S Kumar, A Mohanty, S Maji, S Suryanwanshi, A Rajasubramaniam, S Asthana, M Panda, A K Singh, S P Ganguly, S Shaw, O P Bichhwalia, A K Sahoo, P K Chattopadhyay, N R Chatterjee, K Kundu, C N Das, A K Kannan, R Zorenpuii Zomawia, E Sema, S A Singh, Y I Ghosh, S K Sharma, K Das, B S Choudhuri, T Oncogenesis Short Communication Tumor suppressor p53 is a critical player in the fight against cancer as it controls the cell cycle check point, apoptotic pathways and genomic stability. It is known to be the most frequently mutated gene in a wide variety of human cancers. Single-nucleotide polymorphism of p53 at codon72 leading to substitution of proline (Pro) in place of arginine (Arg) has been identified as a risk factor for development of many cancers, including nasopharyngeal carcinoma (NPC). However, the association of this polymorphism with NPC across the published literature has shown conflicting results. We aimed to conduct a case–control study for a possible relation of p53 codon72 Arg>Pro polymorphism with NPC risk in underdeveloped states of India, combine the result with previously available records from different databases and perform a meta-analysis to draw a more definitive conclusion. A total of 70 NPC patients and 70 healthy controls were enrolled from different hospitals of north-eastern India. The p53 codon72 Arg>Pro polymorphism was typed by polymerase chain reaction, which showed an association with NPC risk. In the meta-analysis consisting of 1842 cases and 2330 controls, it was found that individuals carrying the Pro allele and the ProPro genotype were at a significantly higher risk for NPC as compared with those with the Arg allele and the ArgArg genotype, respectively. Individuals with a ProPro genotype and a combined Pro genotype (ProPro+ArgPro) also showed a significantly higher risk for NPC over a wild homozygote ArgArg genotype. Additionally, the strength of each study was tested by power analysis and genotype distribution by Hardy–Weinberg equilibrium. The outcome of the study indicated that both allele frequency and genotype distribution of p53 codon72 Arg>Pro polymorphism were significantly associated with NPC risk. Stratified analyses based on ethnicity and source of samples supported the above result. Nature Publishing Group 2016-05 2016-05-09 /pmc/articles/PMC4945748/ /pubmed/27159678 http://dx.doi.org/10.1038/oncsis.2016.31 Text en Copyright © 2016 Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Short Communication
Sahu, S K
Chakrabarti, S
Roy, S D
Baishya, N
Reddy, R R
Suklabaidya, S
Kumar, A
Mohanty, S
Maji, S
Suryanwanshi, A
Rajasubramaniam, S
Asthana, M
Panda, A K
Singh, S P
Ganguly, S
Shaw, O P
Bichhwalia, A K
Sahoo, P K
Chattopadhyay, N R
Chatterjee, K
Kundu, C N
Das, A K
Kannan, R
Zorenpuii
Zomawia, E
Sema, S A
Singh, Y I
Ghosh, S K
Sharma, K
Das, B S
Choudhuri, T
Association of p53 codon72 Arg>Pro polymorphism with susceptibility to nasopharyngeal carcinoma: evidence from a case–control study and meta-analysis
title Association of p53 codon72 Arg>Pro polymorphism with susceptibility to nasopharyngeal carcinoma: evidence from a case–control study and meta-analysis
title_full Association of p53 codon72 Arg>Pro polymorphism with susceptibility to nasopharyngeal carcinoma: evidence from a case–control study and meta-analysis
title_fullStr Association of p53 codon72 Arg>Pro polymorphism with susceptibility to nasopharyngeal carcinoma: evidence from a case–control study and meta-analysis
title_full_unstemmed Association of p53 codon72 Arg>Pro polymorphism with susceptibility to nasopharyngeal carcinoma: evidence from a case–control study and meta-analysis
title_short Association of p53 codon72 Arg>Pro polymorphism with susceptibility to nasopharyngeal carcinoma: evidence from a case–control study and meta-analysis
title_sort association of p53 codon72 arg>pro polymorphism with susceptibility to nasopharyngeal carcinoma: evidence from a case–control study and meta-analysis
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4945748/
https://www.ncbi.nlm.nih.gov/pubmed/27159678
http://dx.doi.org/10.1038/oncsis.2016.31
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