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Immunogenicity and protective efficacy of DNA vaccine against visceral leishmaniasis in BALB/c mice
The current study was designed to examine the protective efficacy of DNA vaccines based on gp63 and Hsp70 against murine visceral leishmaniasis. Inbred BALB/c mice were immunized subcutaneously twice at an interval of three weeks with pcDNA3.1(+) encoding T cell epitopes of gp63 and Hsp70 individual...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Editorial Department of Journal of Biomedical Research
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4946321/ https://www.ncbi.nlm.nih.gov/pubmed/27533939 http://dx.doi.org/10.7555/JBR.30.20150125 |
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author | Kaur, Sukhbir Kaur, Tejinder Joshi, Jyoti |
author_facet | Kaur, Sukhbir Kaur, Tejinder Joshi, Jyoti |
author_sort | Kaur, Sukhbir |
collection | PubMed |
description | The current study was designed to examine the protective efficacy of DNA vaccines based on gp63 and Hsp70 against murine visceral leishmaniasis. Inbred BALB/c mice were immunized subcutaneously twice at an interval of three weeks with pcDNA3.1(+) encoding T cell epitopes of gp63 and Hsp70 individually and in combination. Animals were challenged intracardially with 107 promastigotes of Leishmania donovani 10 days post immunization and sacrificed 1, 2 and 3 months post challenge. The immunized animals revealed a significant reduction (P < 0.05) in splenic and hepatic parasite burden as compared to the infected controls. Maximum reduction in parasite load (P < 0.05) was observed in animals treated with a combination of pcDNA/gp63 and pcDNA/Hsp70. These animals also showed heightened DTH response, increased IgG2a, elevated Th1 cytokines (IFN-γ and IL-2) and reduced IgG1 and IL-10 levels. Thus, mice immunized with the cocktail vaccine exhibited significantly greater protection in comparison to those immunized with individual antigens. |
format | Online Article Text |
id | pubmed-4946321 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Editorial Department of Journal of Biomedical Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-49463212016-07-25 Immunogenicity and protective efficacy of DNA vaccine against visceral leishmaniasis in BALB/c mice Kaur, Sukhbir Kaur, Tejinder Joshi, Jyoti J Biomed Res Original Article The current study was designed to examine the protective efficacy of DNA vaccines based on gp63 and Hsp70 against murine visceral leishmaniasis. Inbred BALB/c mice were immunized subcutaneously twice at an interval of three weeks with pcDNA3.1(+) encoding T cell epitopes of gp63 and Hsp70 individually and in combination. Animals were challenged intracardially with 107 promastigotes of Leishmania donovani 10 days post immunization and sacrificed 1, 2 and 3 months post challenge. The immunized animals revealed a significant reduction (P < 0.05) in splenic and hepatic parasite burden as compared to the infected controls. Maximum reduction in parasite load (P < 0.05) was observed in animals treated with a combination of pcDNA/gp63 and pcDNA/Hsp70. These animals also showed heightened DTH response, increased IgG2a, elevated Th1 cytokines (IFN-γ and IL-2) and reduced IgG1 and IL-10 levels. Thus, mice immunized with the cocktail vaccine exhibited significantly greater protection in comparison to those immunized with individual antigens. Editorial Department of Journal of Biomedical Research 2016-07 2016-03-10 /pmc/articles/PMC4946321/ /pubmed/27533939 http://dx.doi.org/10.7555/JBR.30.20150125 Text en © 2016 by the Journal of Biomedical Research. All rights reserved. |
spellingShingle | Original Article Kaur, Sukhbir Kaur, Tejinder Joshi, Jyoti Immunogenicity and protective efficacy of DNA vaccine against visceral leishmaniasis in BALB/c mice |
title | Immunogenicity and protective efficacy of DNA vaccine against visceral leishmaniasis in BALB/c mice |
title_full | Immunogenicity and protective efficacy of DNA vaccine against visceral leishmaniasis in BALB/c mice |
title_fullStr | Immunogenicity and protective efficacy of DNA vaccine against visceral leishmaniasis in BALB/c mice |
title_full_unstemmed | Immunogenicity and protective efficacy of DNA vaccine against visceral leishmaniasis in BALB/c mice |
title_short | Immunogenicity and protective efficacy of DNA vaccine against visceral leishmaniasis in BALB/c mice |
title_sort | immunogenicity and protective efficacy of dna vaccine against visceral leishmaniasis in balb/c mice |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4946321/ https://www.ncbi.nlm.nih.gov/pubmed/27533939 http://dx.doi.org/10.7555/JBR.30.20150125 |
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