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Multiwalled carbon nanotubes intratracheally instilled into the rat lung induce development of pleural malignant mesothelioma and lung tumors

Multiwalled carbon nanotubes (MWCNT) have a fibrous structure and physical properties similar to asbestos and have been shown to induce malignant mesothelioma of the peritoneum after injection into the scrotum or peritoneal cavity in rats and mice. For human cancer risk assessment, however, data aft...

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Autores principales: Suzui, Masumi, Futakuchi, Mitsuru, Fukamachi, Katsumi, Numano, Takamasa, Abdelgied, Mohamed, Takahashi, Satoru, Ohnishi, Makoto, Omori, Toyonori, Tsuruoka, Shuji, Hirose, Akihiko, Kanno, Jun, Sakamoto, Yoshimitsu, Alexander, David B., Alexander, William T., Jiegou, Xu, Tsuda, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4946724/
https://www.ncbi.nlm.nih.gov/pubmed/27098557
http://dx.doi.org/10.1111/cas.12954
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author Suzui, Masumi
Futakuchi, Mitsuru
Fukamachi, Katsumi
Numano, Takamasa
Abdelgied, Mohamed
Takahashi, Satoru
Ohnishi, Makoto
Omori, Toyonori
Tsuruoka, Shuji
Hirose, Akihiko
Kanno, Jun
Sakamoto, Yoshimitsu
Alexander, David B.
Alexander, William T.
Jiegou, Xu
Tsuda, Hiroyuki
author_facet Suzui, Masumi
Futakuchi, Mitsuru
Fukamachi, Katsumi
Numano, Takamasa
Abdelgied, Mohamed
Takahashi, Satoru
Ohnishi, Makoto
Omori, Toyonori
Tsuruoka, Shuji
Hirose, Akihiko
Kanno, Jun
Sakamoto, Yoshimitsu
Alexander, David B.
Alexander, William T.
Jiegou, Xu
Tsuda, Hiroyuki
author_sort Suzui, Masumi
collection PubMed
description Multiwalled carbon nanotubes (MWCNT) have a fibrous structure and physical properties similar to asbestos and have been shown to induce malignant mesothelioma of the peritoneum after injection into the scrotum or peritoneal cavity in rats and mice. For human cancer risk assessment, however, data after administration of MWCNT via the airway, the exposure route that is most relevant to humans, is required. The present study was undertaken to investigate the carcinogenicity of MWCNT‐N (NIKKISO) after administration to the rat lung. MWCNT‐N was fractionated by passing it through a sieve with a pore size of 25 μm. The average lengths of the MWCNT were 4.2 μm before filtration and 2.6 μm in the flow‐through fraction; the length of the retained MWCNT could not be determined. For the present study, 10‐week‐old F344/Crj male rats were divided into five groups: no treatment, vehicle control, MWCNT‐N before filtration, MWCNT‐N flow‐through and MWCNT‐N retained groups. Administration was by the trans‐tracheal intrapulmonary spraying (TIPS) method. Rats were administered a total of 1 mg/rat during the initial 2 weeks of the experiment and then observed up to 109 weeks. The incidences of malignant mesothelioma and lung tumors (bronchiolo‐alveolar adenomas and carcinomas) were 6/38 and 14/38, respectively, in the three groups administered MWCNT and 0/28 and 0/28, respectively, in the control groups. All malignant mesotheliomas were localized in the pericardial pleural cavity. The sieve fractions did not have a significant effect on tumor incidence. In conclusion, administration of MWCNT to the lung in the rat induces malignant mesothelioma and lung tumors.
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spelling pubmed-49467242016-07-27 Multiwalled carbon nanotubes intratracheally instilled into the rat lung induce development of pleural malignant mesothelioma and lung tumors Suzui, Masumi Futakuchi, Mitsuru Fukamachi, Katsumi Numano, Takamasa Abdelgied, Mohamed Takahashi, Satoru Ohnishi, Makoto Omori, Toyonori Tsuruoka, Shuji Hirose, Akihiko Kanno, Jun Sakamoto, Yoshimitsu Alexander, David B. Alexander, William T. Jiegou, Xu Tsuda, Hiroyuki Cancer Sci Original Articles Multiwalled carbon nanotubes (MWCNT) have a fibrous structure and physical properties similar to asbestos and have been shown to induce malignant mesothelioma of the peritoneum after injection into the scrotum or peritoneal cavity in rats and mice. For human cancer risk assessment, however, data after administration of MWCNT via the airway, the exposure route that is most relevant to humans, is required. The present study was undertaken to investigate the carcinogenicity of MWCNT‐N (NIKKISO) after administration to the rat lung. MWCNT‐N was fractionated by passing it through a sieve with a pore size of 25 μm. The average lengths of the MWCNT were 4.2 μm before filtration and 2.6 μm in the flow‐through fraction; the length of the retained MWCNT could not be determined. For the present study, 10‐week‐old F344/Crj male rats were divided into five groups: no treatment, vehicle control, MWCNT‐N before filtration, MWCNT‐N flow‐through and MWCNT‐N retained groups. Administration was by the trans‐tracheal intrapulmonary spraying (TIPS) method. Rats were administered a total of 1 mg/rat during the initial 2 weeks of the experiment and then observed up to 109 weeks. The incidences of malignant mesothelioma and lung tumors (bronchiolo‐alveolar adenomas and carcinomas) were 6/38 and 14/38, respectively, in the three groups administered MWCNT and 0/28 and 0/28, respectively, in the control groups. All malignant mesotheliomas were localized in the pericardial pleural cavity. The sieve fractions did not have a significant effect on tumor incidence. In conclusion, administration of MWCNT to the lung in the rat induces malignant mesothelioma and lung tumors. John Wiley and Sons Inc. 2016-06-23 2016-07 /pmc/articles/PMC4946724/ /pubmed/27098557 http://dx.doi.org/10.1111/cas.12954 Text en © 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial (http://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Suzui, Masumi
Futakuchi, Mitsuru
Fukamachi, Katsumi
Numano, Takamasa
Abdelgied, Mohamed
Takahashi, Satoru
Ohnishi, Makoto
Omori, Toyonori
Tsuruoka, Shuji
Hirose, Akihiko
Kanno, Jun
Sakamoto, Yoshimitsu
Alexander, David B.
Alexander, William T.
Jiegou, Xu
Tsuda, Hiroyuki
Multiwalled carbon nanotubes intratracheally instilled into the rat lung induce development of pleural malignant mesothelioma and lung tumors
title Multiwalled carbon nanotubes intratracheally instilled into the rat lung induce development of pleural malignant mesothelioma and lung tumors
title_full Multiwalled carbon nanotubes intratracheally instilled into the rat lung induce development of pleural malignant mesothelioma and lung tumors
title_fullStr Multiwalled carbon nanotubes intratracheally instilled into the rat lung induce development of pleural malignant mesothelioma and lung tumors
title_full_unstemmed Multiwalled carbon nanotubes intratracheally instilled into the rat lung induce development of pleural malignant mesothelioma and lung tumors
title_short Multiwalled carbon nanotubes intratracheally instilled into the rat lung induce development of pleural malignant mesothelioma and lung tumors
title_sort multiwalled carbon nanotubes intratracheally instilled into the rat lung induce development of pleural malignant mesothelioma and lung tumors
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4946724/
https://www.ncbi.nlm.nih.gov/pubmed/27098557
http://dx.doi.org/10.1111/cas.12954
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