RNA polymerase I–Rrn3 complex at 4.8 Å resolution

Transcription of ribosomal DNA by RNA polymerase I (Pol I) requires the initiation factor Rrn3. Here we report the cryo-EM structure of the Pol I–Rrn3 complex at 4.8 Å resolution. The structure reveals how Rrn3 binding converts an inactive Pol I dimer into an initiation-competent monomeric complex a...

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Autores principales: Engel, Christoph, Plitzko, Jürgen, Cramer, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4947163/
https://www.ncbi.nlm.nih.gov/pubmed/27418309
http://dx.doi.org/10.1038/ncomms12129
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author Engel, Christoph
Plitzko, Jürgen
Cramer, Patrick
author_facet Engel, Christoph
Plitzko, Jürgen
Cramer, Patrick
author_sort Engel, Christoph
collection PubMed
description Transcription of ribosomal DNA by RNA polymerase I (Pol I) requires the initiation factor Rrn3. Here we report the cryo-EM structure of the Pol I–Rrn3 complex at 4.8 Å resolution. The structure reveals how Rrn3 binding converts an inactive Pol I dimer into an initiation-competent monomeric complex and provides insights into the mechanisms of Pol I-specific initiation and regulation.
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spelling pubmed-49471632016-07-27 RNA polymerase I–Rrn3 complex at 4.8 Å resolution Engel, Christoph Plitzko, Jürgen Cramer, Patrick Nat Commun Article Transcription of ribosomal DNA by RNA polymerase I (Pol I) requires the initiation factor Rrn3. Here we report the cryo-EM structure of the Pol I–Rrn3 complex at 4.8 Å resolution. The structure reveals how Rrn3 binding converts an inactive Pol I dimer into an initiation-competent monomeric complex and provides insights into the mechanisms of Pol I-specific initiation and regulation. Nature Publishing Group 2016-07-15 /pmc/articles/PMC4947163/ /pubmed/27418309 http://dx.doi.org/10.1038/ncomms12129 Text en Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Engel, Christoph
Plitzko, Jürgen
Cramer, Patrick
RNA polymerase I–Rrn3 complex at 4.8 Å resolution
title RNA polymerase I–Rrn3 complex at 4.8 Å resolution
title_full RNA polymerase I–Rrn3 complex at 4.8 Å resolution
title_fullStr RNA polymerase I–Rrn3 complex at 4.8 Å resolution
title_full_unstemmed RNA polymerase I–Rrn3 complex at 4.8 Å resolution
title_short RNA polymerase I–Rrn3 complex at 4.8 Å resolution
title_sort rna polymerase i–rrn3 complex at 4.8 å resolution
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4947163/
https://www.ncbi.nlm.nih.gov/pubmed/27418309
http://dx.doi.org/10.1038/ncomms12129
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AT plitzkojurgen rnapolymeraseirrn3complexat48aresolution
AT cramerpatrick rnapolymeraseirrn3complexat48aresolution

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