Mass spectrometry locates local and allosteric conformational changes that occur on cofactor binding

Fdc1 is a decarboxylase enzyme that requires the novel prenylated FMN cofactor for activity. Here, we use it as an exemplar system to show how native top-down and bottom-up mass spectrometry can measure the structural effect of cofactor binding by a protein. For Fdc1(Ubix), the cofactor confers stru...

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Detalles Bibliográficos
Autores principales: Beveridge, Rebecca, Migas, Lukasz G., Payne, Karl A. P., Scrutton, Nigel S., Leys, David, Barran, Perdita E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4947166/
https://www.ncbi.nlm.nih.gov/pubmed/27418477
http://dx.doi.org/10.1038/ncomms12163

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4947166/
https://www.ncbi.nlm.nih.gov/pubmed/27418477
http://dx.doi.org/10.1038/ncomms12163

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