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Septin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV
The accumulation of lipid droplets (LD) is frequently observed in hepatitis C virus (HCV) infection and represents an important risk factor for the development of liver steatosis and cirrhosis. The mechanisms of LD biogenesis and growth remain open questions. Here, transcriptome analysis reveals a s...
| Autores principales: | , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2016
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4947189/ https://www.ncbi.nlm.nih.gov/pubmed/27417143 http://dx.doi.org/10.1038/ncomms12203 |
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| author | Akil, Abdellah Peng, Juan Omrane, Mohyeddine Gondeau, Claire Desterke, Christophe Marin, Mickaël Tronchère, Hélène Taveneau, Cyntia Sar, Sokhavuth Briolotti, Philippe Benjelloun, Soumaya Benjouad, Abdelaziz Maurel, Patrick Thiers, Valérie Bressanelli, Stéphane Samuel, Didier Bréchot, Christian Gassama-Diagne, Ama |
| author_facet | Akil, Abdellah Peng, Juan Omrane, Mohyeddine Gondeau, Claire Desterke, Christophe Marin, Mickaël Tronchère, Hélène Taveneau, Cyntia Sar, Sokhavuth Briolotti, Philippe Benjelloun, Soumaya Benjouad, Abdelaziz Maurel, Patrick Thiers, Valérie Bressanelli, Stéphane Samuel, Didier Bréchot, Christian Gassama-Diagne, Ama |
| author_sort | Akil, Abdellah |
| collection | PubMed |
| description | The accumulation of lipid droplets (LD) is frequently observed in hepatitis C virus (HCV) infection and represents an important risk factor for the development of liver steatosis and cirrhosis. The mechanisms of LD biogenesis and growth remain open questions. Here, transcriptome analysis reveals a significant upregulation of septin 9 in HCV-induced cirrhosis compared with the normal liver. HCV infection increases septin 9 expression and induces its assembly into filaments. Septin 9 regulates LD growth and perinuclear accumulation in a manner dependent on dynamic microtubules. The effects of septin 9 on LDs are also dependent on binding to PtdIns5P, which, in turn, controls the formation of septin 9 filaments and its interaction with microtubules. This previously undescribed cooperation between PtdIns5P and septin 9 regulates oleate-induced accumulation of LDs. Overall, our data offer a novel route for LD growth through the involvement of a septin 9/PtdIns5P signalling pathway. |
| format | Online Article Text |
| id | pubmed-4947189 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2016 |
| publisher | Nature Publishing Group |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-49471892016-07-27 Septin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV Akil, Abdellah Peng, Juan Omrane, Mohyeddine Gondeau, Claire Desterke, Christophe Marin, Mickaël Tronchère, Hélène Taveneau, Cyntia Sar, Sokhavuth Briolotti, Philippe Benjelloun, Soumaya Benjouad, Abdelaziz Maurel, Patrick Thiers, Valérie Bressanelli, Stéphane Samuel, Didier Bréchot, Christian Gassama-Diagne, Ama Nat Commun Article The accumulation of lipid droplets (LD) is frequently observed in hepatitis C virus (HCV) infection and represents an important risk factor for the development of liver steatosis and cirrhosis. The mechanisms of LD biogenesis and growth remain open questions. Here, transcriptome analysis reveals a significant upregulation of septin 9 in HCV-induced cirrhosis compared with the normal liver. HCV infection increases septin 9 expression and induces its assembly into filaments. Septin 9 regulates LD growth and perinuclear accumulation in a manner dependent on dynamic microtubules. The effects of septin 9 on LDs are also dependent on binding to PtdIns5P, which, in turn, controls the formation of septin 9 filaments and its interaction with microtubules. This previously undescribed cooperation between PtdIns5P and septin 9 regulates oleate-induced accumulation of LDs. Overall, our data offer a novel route for LD growth through the involvement of a septin 9/PtdIns5P signalling pathway. Nature Publishing Group 2016-07-15 /pmc/articles/PMC4947189/ /pubmed/27417143 http://dx.doi.org/10.1038/ncomms12203 Text en Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| spellingShingle | Article Akil, Abdellah Peng, Juan Omrane, Mohyeddine Gondeau, Claire Desterke, Christophe Marin, Mickaël Tronchère, Hélène Taveneau, Cyntia Sar, Sokhavuth Briolotti, Philippe Benjelloun, Soumaya Benjouad, Abdelaziz Maurel, Patrick Thiers, Valérie Bressanelli, Stéphane Samuel, Didier Bréchot, Christian Gassama-Diagne, Ama Septin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV |
| title | Septin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV |
| title_full | Septin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV |
| title_fullStr | Septin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV |
| title_full_unstemmed | Septin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV |
| title_short | Septin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV |
| title_sort | septin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by hcv |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4947189/ https://www.ncbi.nlm.nih.gov/pubmed/27417143 http://dx.doi.org/10.1038/ncomms12203 |
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