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Effect of astragalus injection on renal tubular epithelial transdifferentiation in type 2 diabetic mice
BACKGROUND: Astragalus injection is used by practitioners of traditional Chinese medicine to treat diabetic nephropathy (DN). The current study was conducted to determine the effect of astragalus on tubular epithelial transdifferentiation during the progression of DN in KKAy mice, as well as to inve...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4947258/ https://www.ncbi.nlm.nih.gov/pubmed/27422712 http://dx.doi.org/10.1186/s12906-016-1208-8 |
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author | Yi, Yue-e Li, Shu-yu Nie, Yan-na Jia, De-xian Zhang, Zhi-hui Wang, Yan-fei Wang, Qian |
author_facet | Yi, Yue-e Li, Shu-yu Nie, Yan-na Jia, De-xian Zhang, Zhi-hui Wang, Yan-fei Wang, Qian |
author_sort | Yi, Yue-e |
collection | PubMed |
description | BACKGROUND: Astragalus injection is used by practitioners of traditional Chinese medicine to treat diabetic nephropathy (DN). The current study was conducted to determine the effect of astragalus on tubular epithelial transdifferentiation during the progression of DN in KKAy mice, as well as to investigate the molecular mechanism underlying this effect. METHODS: Diabetic, 14-week-old, male KKAy mice were randomly divided into a model group and an astragalus treatment group, while age-matched male C57BL/6 J mice were selected as controls. The treatment group received daily intraperitoneal injections of astragalus (0.03 mL/10 g per day), while the model group received injections of an equal volume of saline. Mice were euthanized after 24 weeks. Serum samples were obtained from the animals in each group for blood glucose measurement. Kidney tissue samples were used for morphometric studies. The mRNA and protein expression levels of transforming growth factor beta 1 (TGF-β1), transforming growth factor beta receptor 1 (TGFβ-R1), alpha smooth muscle actin (α-SMA), and E-cadherin were evaluated using real-time polymerase chain reaction (PCR) and western blotting. RESULTS: Astragalus significantly reduced blood glucose levels; inhibited morphological changes in the kidneys of KKAy mice; reduced mRNA and protein expression levels of TGF-β1, TGFβ-R1, and α-SMA; and increased E-cadherin expression. CONCLUSIONS: Tubular epithelial transdifferentiation plays an important role in the development of DN in diabetic mice. Administration of astragalus likely prevents or mitigates DN by suppressing tubular epithelial transdifferentiation, protecting KKAy mice from renal damage. |
format | Online Article Text |
id | pubmed-4947258 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-49472582016-07-17 Effect of astragalus injection on renal tubular epithelial transdifferentiation in type 2 diabetic mice Yi, Yue-e Li, Shu-yu Nie, Yan-na Jia, De-xian Zhang, Zhi-hui Wang, Yan-fei Wang, Qian BMC Complement Altern Med Research Article BACKGROUND: Astragalus injection is used by practitioners of traditional Chinese medicine to treat diabetic nephropathy (DN). The current study was conducted to determine the effect of astragalus on tubular epithelial transdifferentiation during the progression of DN in KKAy mice, as well as to investigate the molecular mechanism underlying this effect. METHODS: Diabetic, 14-week-old, male KKAy mice were randomly divided into a model group and an astragalus treatment group, while age-matched male C57BL/6 J mice were selected as controls. The treatment group received daily intraperitoneal injections of astragalus (0.03 mL/10 g per day), while the model group received injections of an equal volume of saline. Mice were euthanized after 24 weeks. Serum samples were obtained from the animals in each group for blood glucose measurement. Kidney tissue samples were used for morphometric studies. The mRNA and protein expression levels of transforming growth factor beta 1 (TGF-β1), transforming growth factor beta receptor 1 (TGFβ-R1), alpha smooth muscle actin (α-SMA), and E-cadherin were evaluated using real-time polymerase chain reaction (PCR) and western blotting. RESULTS: Astragalus significantly reduced blood glucose levels; inhibited morphological changes in the kidneys of KKAy mice; reduced mRNA and protein expression levels of TGF-β1, TGFβ-R1, and α-SMA; and increased E-cadherin expression. CONCLUSIONS: Tubular epithelial transdifferentiation plays an important role in the development of DN in diabetic mice. Administration of astragalus likely prevents or mitigates DN by suppressing tubular epithelial transdifferentiation, protecting KKAy mice from renal damage. BioMed Central 2016-07-16 /pmc/articles/PMC4947258/ /pubmed/27422712 http://dx.doi.org/10.1186/s12906-016-1208-8 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Yi, Yue-e Li, Shu-yu Nie, Yan-na Jia, De-xian Zhang, Zhi-hui Wang, Yan-fei Wang, Qian Effect of astragalus injection on renal tubular epithelial transdifferentiation in type 2 diabetic mice |
title | Effect of astragalus injection on renal tubular epithelial transdifferentiation in type 2 diabetic mice |
title_full | Effect of astragalus injection on renal tubular epithelial transdifferentiation in type 2 diabetic mice |
title_fullStr | Effect of astragalus injection on renal tubular epithelial transdifferentiation in type 2 diabetic mice |
title_full_unstemmed | Effect of astragalus injection on renal tubular epithelial transdifferentiation in type 2 diabetic mice |
title_short | Effect of astragalus injection on renal tubular epithelial transdifferentiation in type 2 diabetic mice |
title_sort | effect of astragalus injection on renal tubular epithelial transdifferentiation in type 2 diabetic mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4947258/ https://www.ncbi.nlm.nih.gov/pubmed/27422712 http://dx.doi.org/10.1186/s12906-016-1208-8 |
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