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Transcriptome Analysis of B Cell Immune Functions in Periodontitis: Mucosal Tissue Responses to the Oral Microbiome in Aging
Evidence has shown activation of T and B cells in gingival tissues in experimental models and in humans diagnosed with periodontitis. The results of this adaptive immune response are noted both locally and systemically with antigenic specificity for an array of oral bacteria, including periodontopat...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4947588/ https://www.ncbi.nlm.nih.gov/pubmed/27486459 http://dx.doi.org/10.3389/fimmu.2016.00272 |
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author | Ebersole, Jeffrey L. Kirakodu, Sreenatha S. Novak, M. John Orraca, Luis Martinez, Janis Gonzalez Cunningham, Larry L. Thomas, Mark V. Stromberg, Arnold Pandruvada, Subramanya N. Gonzalez, Octavio A. |
author_facet | Ebersole, Jeffrey L. Kirakodu, Sreenatha S. Novak, M. John Orraca, Luis Martinez, Janis Gonzalez Cunningham, Larry L. Thomas, Mark V. Stromberg, Arnold Pandruvada, Subramanya N. Gonzalez, Octavio A. |
author_sort | Ebersole, Jeffrey L. |
collection | PubMed |
description | Evidence has shown activation of T and B cells in gingival tissues in experimental models and in humans diagnosed with periodontitis. The results of this adaptive immune response are noted both locally and systemically with antigenic specificity for an array of oral bacteria, including periodontopathic species, e.g., Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. It has been recognized through epidemiological studies and clinical observations that the prevalence of periodontitis increases with age. This report describes our studies evaluating gingival tissue transcriptomes in humans and specifically exploiting the use of a non-human primate model of naturally occurring periodontitis to delineate gingival mucosal tissue gene expression profiles focusing on cells/genes critical for the development of humoral adaptive immune responses. Patterns of B cell and plasmacyte genes were altered in aging healthy gingival tissues. Substantial increases in a large number of genes reflecting antigen-dependent activation, B cell activation, B cell proliferation, and B cell differentiation/maturation were observed in periodontitis in adults and aged animals. Finally, evaluation of the relationship of these gene expression patterns with those of various tissue destructive molecules (MMP2, MMP9, CTSK, TNFα, and RANKL) showed a greater frequency of positive correlations in healthy tissues versus periodontitis tissues, with only MMP9 correlations similar between the two tissue types. These results are consistent with B cell response activities in healthy tissues potentially contributing to muting the effects of the tissue destructive biomolecules, whereas with periodontitis this relationship is adversely affected and enabling a progression of tissue destructive events. |
format | Online Article Text |
id | pubmed-4947588 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-49475882016-08-02 Transcriptome Analysis of B Cell Immune Functions in Periodontitis: Mucosal Tissue Responses to the Oral Microbiome in Aging Ebersole, Jeffrey L. Kirakodu, Sreenatha S. Novak, M. John Orraca, Luis Martinez, Janis Gonzalez Cunningham, Larry L. Thomas, Mark V. Stromberg, Arnold Pandruvada, Subramanya N. Gonzalez, Octavio A. Front Immunol Immunology Evidence has shown activation of T and B cells in gingival tissues in experimental models and in humans diagnosed with periodontitis. The results of this adaptive immune response are noted both locally and systemically with antigenic specificity for an array of oral bacteria, including periodontopathic species, e.g., Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. It has been recognized through epidemiological studies and clinical observations that the prevalence of periodontitis increases with age. This report describes our studies evaluating gingival tissue transcriptomes in humans and specifically exploiting the use of a non-human primate model of naturally occurring periodontitis to delineate gingival mucosal tissue gene expression profiles focusing on cells/genes critical for the development of humoral adaptive immune responses. Patterns of B cell and plasmacyte genes were altered in aging healthy gingival tissues. Substantial increases in a large number of genes reflecting antigen-dependent activation, B cell activation, B cell proliferation, and B cell differentiation/maturation were observed in periodontitis in adults and aged animals. Finally, evaluation of the relationship of these gene expression patterns with those of various tissue destructive molecules (MMP2, MMP9, CTSK, TNFα, and RANKL) showed a greater frequency of positive correlations in healthy tissues versus periodontitis tissues, with only MMP9 correlations similar between the two tissue types. These results are consistent with B cell response activities in healthy tissues potentially contributing to muting the effects of the tissue destructive biomolecules, whereas with periodontitis this relationship is adversely affected and enabling a progression of tissue destructive events. Frontiers Media S.A. 2016-07-18 /pmc/articles/PMC4947588/ /pubmed/27486459 http://dx.doi.org/10.3389/fimmu.2016.00272 Text en Copyright © 2016 Ebersole, Kirakodu, Novak, Orraca, Martinez, Cunningham, Thomas, Stromberg, Pandruvada and Gonzalez. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Ebersole, Jeffrey L. Kirakodu, Sreenatha S. Novak, M. John Orraca, Luis Martinez, Janis Gonzalez Cunningham, Larry L. Thomas, Mark V. Stromberg, Arnold Pandruvada, Subramanya N. Gonzalez, Octavio A. Transcriptome Analysis of B Cell Immune Functions in Periodontitis: Mucosal Tissue Responses to the Oral Microbiome in Aging |
title | Transcriptome Analysis of B Cell Immune Functions in Periodontitis: Mucosal Tissue Responses to the Oral Microbiome in Aging |
title_full | Transcriptome Analysis of B Cell Immune Functions in Periodontitis: Mucosal Tissue Responses to the Oral Microbiome in Aging |
title_fullStr | Transcriptome Analysis of B Cell Immune Functions in Periodontitis: Mucosal Tissue Responses to the Oral Microbiome in Aging |
title_full_unstemmed | Transcriptome Analysis of B Cell Immune Functions in Periodontitis: Mucosal Tissue Responses to the Oral Microbiome in Aging |
title_short | Transcriptome Analysis of B Cell Immune Functions in Periodontitis: Mucosal Tissue Responses to the Oral Microbiome in Aging |
title_sort | transcriptome analysis of b cell immune functions in periodontitis: mucosal tissue responses to the oral microbiome in aging |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4947588/ https://www.ncbi.nlm.nih.gov/pubmed/27486459 http://dx.doi.org/10.3389/fimmu.2016.00272 |
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