Modular, Antibody-free Time-Resolved LRET Kinase Assay Enabled by Quantum Dots and Tb(3+)-sensitizing Peptides

Fluorescent drug screening assays are essential for tyrosine kinase inhibitor discovery. Here we demonstrate a flexible, antibody-free TR-LRET kinase assay strategy that is enabled by the combination of streptavidin-coated quantum dot (QD) acceptors and biotinylated, Tb(3+) sensitizing peptide donor...

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Detalles Bibliográficos
Autores principales: Cui, Wei, Parker, Laurie L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4947905/
https://www.ncbi.nlm.nih.gov/pubmed/27426233
http://dx.doi.org/10.1038/srep28971

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4947905/
https://www.ncbi.nlm.nih.gov/pubmed/27426233
http://dx.doi.org/10.1038/srep28971

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