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Glaucoma related Proteomic Alterations in Human Retina Samples

Glaucoma related proteomic changes have been documented in cell and animal models. However, proteomic studies investigating on human retina samples are still rare. In the present work, retina samples of glaucoma and non-glaucoma control donors have been examined by a state-of-the-art mass spectromet...

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Autores principales: Funke, Sebastian, Perumal, Natarajan, Beck, Sabine, Gabel-Scheurich, Silke, Schmelter, Carsten, Teister, Julia, Gerbig, Claudia, Gramlich, Oliver W., Pfeiffer, Norbert, Grus, Franz H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4947915/
https://www.ncbi.nlm.nih.gov/pubmed/27425789
http://dx.doi.org/10.1038/srep29759
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author Funke, Sebastian
Perumal, Natarajan
Beck, Sabine
Gabel-Scheurich, Silke
Schmelter, Carsten
Teister, Julia
Gerbig, Claudia
Gramlich, Oliver W.
Pfeiffer, Norbert
Grus, Franz H.
author_facet Funke, Sebastian
Perumal, Natarajan
Beck, Sabine
Gabel-Scheurich, Silke
Schmelter, Carsten
Teister, Julia
Gerbig, Claudia
Gramlich, Oliver W.
Pfeiffer, Norbert
Grus, Franz H.
author_sort Funke, Sebastian
collection PubMed
description Glaucoma related proteomic changes have been documented in cell and animal models. However, proteomic studies investigating on human retina samples are still rare. In the present work, retina samples of glaucoma and non-glaucoma control donors have been examined by a state-of-the-art mass spectrometry (MS) workflow to uncover glaucoma related proteomic changes. More than 600 proteins could be identified with high confidence (FDR < 1%) in human retina samples. Distinct proteomic changes have been observed in 10% of proteins encircling mitochondrial and nucleus species. Numerous proteins showed a significant glaucoma related level change (p < 0.05) or distinct tendency of alteration (p < 0.1). Candidates were documented to be involved in cellular development, stress and cell death. Increase of stress related proteins and decrease of new glaucoma related candidates, ADP/ATP translocase 3 (ANT3), PC4 and SRFS1-interacting protein 1 (DFS70) and methyl-CpG-binding protein 2 (MeCp2) could be documented by MS. Moreover, candidates could be validated by Accurate Inclusion Mass Screening (AIMS) and immunostaining and supported for the retinal ganglion cell layer (GCL) by laser capture microdissection (LCM) in porcine and human eye cryosections. The workflow allowed a detailed view into the human retina proteome highlighting new molecular players ANT3, DFS70 and MeCp2 associated to glaucoma.
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spelling pubmed-49479152016-07-26 Glaucoma related Proteomic Alterations in Human Retina Samples Funke, Sebastian Perumal, Natarajan Beck, Sabine Gabel-Scheurich, Silke Schmelter, Carsten Teister, Julia Gerbig, Claudia Gramlich, Oliver W. Pfeiffer, Norbert Grus, Franz H. Sci Rep Article Glaucoma related proteomic changes have been documented in cell and animal models. However, proteomic studies investigating on human retina samples are still rare. In the present work, retina samples of glaucoma and non-glaucoma control donors have been examined by a state-of-the-art mass spectrometry (MS) workflow to uncover glaucoma related proteomic changes. More than 600 proteins could be identified with high confidence (FDR < 1%) in human retina samples. Distinct proteomic changes have been observed in 10% of proteins encircling mitochondrial and nucleus species. Numerous proteins showed a significant glaucoma related level change (p < 0.05) or distinct tendency of alteration (p < 0.1). Candidates were documented to be involved in cellular development, stress and cell death. Increase of stress related proteins and decrease of new glaucoma related candidates, ADP/ATP translocase 3 (ANT3), PC4 and SRFS1-interacting protein 1 (DFS70) and methyl-CpG-binding protein 2 (MeCp2) could be documented by MS. Moreover, candidates could be validated by Accurate Inclusion Mass Screening (AIMS) and immunostaining and supported for the retinal ganglion cell layer (GCL) by laser capture microdissection (LCM) in porcine and human eye cryosections. The workflow allowed a detailed view into the human retina proteome highlighting new molecular players ANT3, DFS70 and MeCp2 associated to glaucoma. Nature Publishing Group 2016-07-18 /pmc/articles/PMC4947915/ /pubmed/27425789 http://dx.doi.org/10.1038/srep29759 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Funke, Sebastian
Perumal, Natarajan
Beck, Sabine
Gabel-Scheurich, Silke
Schmelter, Carsten
Teister, Julia
Gerbig, Claudia
Gramlich, Oliver W.
Pfeiffer, Norbert
Grus, Franz H.
Glaucoma related Proteomic Alterations in Human Retina Samples
title Glaucoma related Proteomic Alterations in Human Retina Samples
title_full Glaucoma related Proteomic Alterations in Human Retina Samples
title_fullStr Glaucoma related Proteomic Alterations in Human Retina Samples
title_full_unstemmed Glaucoma related Proteomic Alterations in Human Retina Samples
title_short Glaucoma related Proteomic Alterations in Human Retina Samples
title_sort glaucoma related proteomic alterations in human retina samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4947915/
https://www.ncbi.nlm.nih.gov/pubmed/27425789
http://dx.doi.org/10.1038/srep29759
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