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Thymosin From Bombyx mori Is Down-Regulated in Expression by BmNPV Exhibiting Antiviral Activity

Thymosins have been highly conserved during evolution. These hormones exist in many animal species and play an essential role in many biological events. However, little is known regarding the physiological function of silkworm Bombyx mori thymosin (BmTHY). In this study, we investigated the expressi...

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Autores principales: Zhang, Chen, Wang, Yongdi, Fang, Qiang, Xu, Minlin, Lv, Mengyuan, Liao, Jinxu, Li, Si, Nie, Zuoming, Zhang, Wenping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4948601/
https://www.ncbi.nlm.nih.gov/pubmed/27432352
http://dx.doi.org/10.1093/jisesa/iew039
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author Zhang, Chen
Wang, Yongdi
Fang, Qiang
Xu, Minlin
Lv, Mengyuan
Liao, Jinxu
Li, Si
Nie, Zuoming
Zhang, Wenping
author_facet Zhang, Chen
Wang, Yongdi
Fang, Qiang
Xu, Minlin
Lv, Mengyuan
Liao, Jinxu
Li, Si
Nie, Zuoming
Zhang, Wenping
author_sort Zhang, Chen
collection PubMed
description Thymosins have been highly conserved during evolution. These hormones exist in many animal species and play an essential role in many biological events. However, little is known regarding the physiological function of silkworm Bombyx mori thymosin (BmTHY). In this study, we investigated the expression pattern of BmTHY in a Bombyx mori larval ovarian cell line (BmN) challenged with Bombyx mori nuclear polyhydrosis virus (BmNPV) and the antiviral effect of recombinant BmTHY (rBmTHY) for Bombyx mori against BmNPV. Western-blot assay and qRT-PCR analysis revealed that the level of BmTHY protein expression and transcription decreased over time when BmN cells were infected by BmNPV. Treatment with endotoxin-free rBmTHY led to a significant reduction in viral titer in the supernatant of BmN cells challenged with BmNPV. The results from antiviral tests performed in vitro and in vivo showed that endotoxin-free rBmTHY improved the survival rate of Bombyx mori infected with BmNPV. These findings suggest that BmTHY exerts immunomodulatory effects on Bombyx mori, rendering them resistant to viral infection.
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spelling pubmed-49486012016-07-20 Thymosin From Bombyx mori Is Down-Regulated in Expression by BmNPV Exhibiting Antiviral Activity Zhang, Chen Wang, Yongdi Fang, Qiang Xu, Minlin Lv, Mengyuan Liao, Jinxu Li, Si Nie, Zuoming Zhang, Wenping J Insect Sci Research Article Thymosins have been highly conserved during evolution. These hormones exist in many animal species and play an essential role in many biological events. However, little is known regarding the physiological function of silkworm Bombyx mori thymosin (BmTHY). In this study, we investigated the expression pattern of BmTHY in a Bombyx mori larval ovarian cell line (BmN) challenged with Bombyx mori nuclear polyhydrosis virus (BmNPV) and the antiviral effect of recombinant BmTHY (rBmTHY) for Bombyx mori against BmNPV. Western-blot assay and qRT-PCR analysis revealed that the level of BmTHY protein expression and transcription decreased over time when BmN cells were infected by BmNPV. Treatment with endotoxin-free rBmTHY led to a significant reduction in viral titer in the supernatant of BmN cells challenged with BmNPV. The results from antiviral tests performed in vitro and in vivo showed that endotoxin-free rBmTHY improved the survival rate of Bombyx mori infected with BmNPV. These findings suggest that BmTHY exerts immunomodulatory effects on Bombyx mori, rendering them resistant to viral infection. Oxford University Press 2016-07-18 /pmc/articles/PMC4948601/ /pubmed/27432352 http://dx.doi.org/10.1093/jisesa/iew039 Text en © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Article
Zhang, Chen
Wang, Yongdi
Fang, Qiang
Xu, Minlin
Lv, Mengyuan
Liao, Jinxu
Li, Si
Nie, Zuoming
Zhang, Wenping
Thymosin From Bombyx mori Is Down-Regulated in Expression by BmNPV Exhibiting Antiviral Activity
title Thymosin From Bombyx mori Is Down-Regulated in Expression by BmNPV Exhibiting Antiviral Activity
title_full Thymosin From Bombyx mori Is Down-Regulated in Expression by BmNPV Exhibiting Antiviral Activity
title_fullStr Thymosin From Bombyx mori Is Down-Regulated in Expression by BmNPV Exhibiting Antiviral Activity
title_full_unstemmed Thymosin From Bombyx mori Is Down-Regulated in Expression by BmNPV Exhibiting Antiviral Activity
title_short Thymosin From Bombyx mori Is Down-Regulated in Expression by BmNPV Exhibiting Antiviral Activity
title_sort thymosin from bombyx mori is down-regulated in expression by bmnpv exhibiting antiviral activity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4948601/
https://www.ncbi.nlm.nih.gov/pubmed/27432352
http://dx.doi.org/10.1093/jisesa/iew039
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