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Differential Transcriptional Changes in Mice Exposed to Chemically Distinct Diesel Samples

Epidemiological studies have linked exposure to ambient particulate matter (PM) with increased asthmatic symptoms. Diesel exhaust particles (DEP) are a predominant source of vehicle derived ambient PM, and experimental studies have demonstrated that they may have adjuvant potential when given with a...

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Autores principales: Stevens, Tina, Hester, Susan, Gilmour, M. Ian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Libertas Academica 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4948654/
https://www.ncbi.nlm.nih.gov/pubmed/27458330
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author Stevens, Tina
Hester, Susan
Gilmour, M. Ian
author_facet Stevens, Tina
Hester, Susan
Gilmour, M. Ian
author_sort Stevens, Tina
collection PubMed
description Epidemiological studies have linked exposure to ambient particulate matter (PM) with increased asthmatic symptoms. Diesel exhaust particles (DEP) are a predominant source of vehicle derived ambient PM, and experimental studies have demonstrated that they may have adjuvant potential when given with an antigen. We previously compared 3 DEP samples: N-DEP, A-DEP, and C-DEP in a murine ovalbumin (OVA) mucosal sensitization model and reported the adjuvant activity to be: C-DEP ≈ A-DEP > N-DEP. The present study analyzed gene expression changes from the lungs of these mice. Transcription profiling demonstrated that all the DEP samples altered cytokine and toll-like receptor pathways regardless of type, with or without antigen sensitization. Further analysis of DEP exposure with OVA showed that all DEP treatments altered networks involved in immune and inflammatory responses. The A- and C-DEP/OVA treatments induced differential expression of apoptosis pathways in association with stronger adjuvant responses, while expression of cell cycle control and DNA damage pathways were also altered in the C-DEP/OVA treatment. This comprehensive approach using gene expression analysis to examine changes at a pathway level provides detailed information on events occurring in the lung after DEP exposure, and confirms that the most bioactive sample induced many more individual genes and changes in immunoregulatory and homeostatic pathways.
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spelling pubmed-49486542016-07-25 Differential Transcriptional Changes in Mice Exposed to Chemically Distinct Diesel Samples Stevens, Tina Hester, Susan Gilmour, M. Ian Biomed Inform Insights Original Research Epidemiological studies have linked exposure to ambient particulate matter (PM) with increased asthmatic symptoms. Diesel exhaust particles (DEP) are a predominant source of vehicle derived ambient PM, and experimental studies have demonstrated that they may have adjuvant potential when given with an antigen. We previously compared 3 DEP samples: N-DEP, A-DEP, and C-DEP in a murine ovalbumin (OVA) mucosal sensitization model and reported the adjuvant activity to be: C-DEP ≈ A-DEP > N-DEP. The present study analyzed gene expression changes from the lungs of these mice. Transcription profiling demonstrated that all the DEP samples altered cytokine and toll-like receptor pathways regardless of type, with or without antigen sensitization. Further analysis of DEP exposure with OVA showed that all DEP treatments altered networks involved in immune and inflammatory responses. The A- and C-DEP/OVA treatments induced differential expression of apoptosis pathways in association with stronger adjuvant responses, while expression of cell cycle control and DNA damage pathways were also altered in the C-DEP/OVA treatment. This comprehensive approach using gene expression analysis to examine changes at a pathway level provides detailed information on events occurring in the lung after DEP exposure, and confirms that the most bioactive sample induced many more individual genes and changes in immunoregulatory and homeostatic pathways. Libertas Academica 2010-08-12 /pmc/articles/PMC4948654/ /pubmed/27458330 Text en © 2010 the author(s), publisher and licensee Libertas Academica Ltd. This is an open access article. Unrestricted non-commercial use is permitted provided the original work is properly cited.
spellingShingle Original Research
Stevens, Tina
Hester, Susan
Gilmour, M. Ian
Differential Transcriptional Changes in Mice Exposed to Chemically Distinct Diesel Samples
title Differential Transcriptional Changes in Mice Exposed to Chemically Distinct Diesel Samples
title_full Differential Transcriptional Changes in Mice Exposed to Chemically Distinct Diesel Samples
title_fullStr Differential Transcriptional Changes in Mice Exposed to Chemically Distinct Diesel Samples
title_full_unstemmed Differential Transcriptional Changes in Mice Exposed to Chemically Distinct Diesel Samples
title_short Differential Transcriptional Changes in Mice Exposed to Chemically Distinct Diesel Samples
title_sort differential transcriptional changes in mice exposed to chemically distinct diesel samples
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4948654/
https://www.ncbi.nlm.nih.gov/pubmed/27458330
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