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Effect of Acteoside as a Cell Protector to Produce a Cloned Dog
Somatic cell nuclear transfer (SCNT) is a well-known laboratory technique. The principle of the SCNT involves the reprogramming a somatic nucleus by injecting a somatic cell into a recipient oocyte whose nucleus has been removed. Therefore, the nucleus donor cells are considered as a crucial factor...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4948914/ https://www.ncbi.nlm.nih.gov/pubmed/27428333 http://dx.doi.org/10.1371/journal.pone.0159330 |
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author | Lee, Ji Hye Chun, Ju Lan Kim, Keun Jung Kim, Eun Young Kim, Dong-hee Lee, Bo Myeong Han, Kil Woo Park, Kang-Sun Lee, Kyung-Bon Kim, Min Kyu |
author_facet | Lee, Ji Hye Chun, Ju Lan Kim, Keun Jung Kim, Eun Young Kim, Dong-hee Lee, Bo Myeong Han, Kil Woo Park, Kang-Sun Lee, Kyung-Bon Kim, Min Kyu |
author_sort | Lee, Ji Hye |
collection | PubMed |
description | Somatic cell nuclear transfer (SCNT) is a well-known laboratory technique. The principle of the SCNT involves the reprogramming a somatic nucleus by injecting a somatic cell into a recipient oocyte whose nucleus has been removed. Therefore, the nucleus donor cells are considered as a crucial factor in SCNT. Cell cycle synchronization of nucleus donor cells at G0/G1 stage can be induced by contact inhibition or serum starvation. In this study, acteoside, a phenylpropanoid glycoside compound, was investigated to determine whether it is applicable for inducing cell cycle synchronization, cytoprotection, and improving SCNT efficiency in canine fetal fibroblasts. Primary canine fetal fibroblasts were treated with acteoside (10, 30, 50 μM) for various time periods (24, 48 and 72 hours). Cell cycle synchronization at G0/G1 stage did not differ significantly with the method of induction: acteoside treatment, contact inhibition or serum starvation. However, of these three treatments, serum starvation resulted in significantly increased level of reactive oxygen species (ROS) (99.5 ± 0.3%) and apoptosis. The results also revealed that acteoside reduced ROS and apoptosis processes including necrosis in canine fetal fibroblasts, and improved the cell survival. Canine fetal fibroblasts treated with acteoside were successfully arrested at the G0/G1 stage. Moreover, the reconstructed embryos using nucleus donor cells treated with acteoside produced a healthy cloned dog, but not the embryos produced using nucleus donor cells subjected to contact inhibition. In conclusion, acteoside induced cell cycle synchronization of nucleus donor cells would be an alternative method to improve the efficiency of canine SCNT because of its cytoprotective effects. |
format | Online Article Text |
id | pubmed-4948914 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-49489142016-08-01 Effect of Acteoside as a Cell Protector to Produce a Cloned Dog Lee, Ji Hye Chun, Ju Lan Kim, Keun Jung Kim, Eun Young Kim, Dong-hee Lee, Bo Myeong Han, Kil Woo Park, Kang-Sun Lee, Kyung-Bon Kim, Min Kyu PLoS One Research Article Somatic cell nuclear transfer (SCNT) is a well-known laboratory technique. The principle of the SCNT involves the reprogramming a somatic nucleus by injecting a somatic cell into a recipient oocyte whose nucleus has been removed. Therefore, the nucleus donor cells are considered as a crucial factor in SCNT. Cell cycle synchronization of nucleus donor cells at G0/G1 stage can be induced by contact inhibition or serum starvation. In this study, acteoside, a phenylpropanoid glycoside compound, was investigated to determine whether it is applicable for inducing cell cycle synchronization, cytoprotection, and improving SCNT efficiency in canine fetal fibroblasts. Primary canine fetal fibroblasts were treated with acteoside (10, 30, 50 μM) for various time periods (24, 48 and 72 hours). Cell cycle synchronization at G0/G1 stage did not differ significantly with the method of induction: acteoside treatment, contact inhibition or serum starvation. However, of these three treatments, serum starvation resulted in significantly increased level of reactive oxygen species (ROS) (99.5 ± 0.3%) and apoptosis. The results also revealed that acteoside reduced ROS and apoptosis processes including necrosis in canine fetal fibroblasts, and improved the cell survival. Canine fetal fibroblasts treated with acteoside were successfully arrested at the G0/G1 stage. Moreover, the reconstructed embryos using nucleus donor cells treated with acteoside produced a healthy cloned dog, but not the embryos produced using nucleus donor cells subjected to contact inhibition. In conclusion, acteoside induced cell cycle synchronization of nucleus donor cells would be an alternative method to improve the efficiency of canine SCNT because of its cytoprotective effects. Public Library of Science 2016-07-18 /pmc/articles/PMC4948914/ /pubmed/27428333 http://dx.doi.org/10.1371/journal.pone.0159330 Text en © 2016 Lee et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Lee, Ji Hye Chun, Ju Lan Kim, Keun Jung Kim, Eun Young Kim, Dong-hee Lee, Bo Myeong Han, Kil Woo Park, Kang-Sun Lee, Kyung-Bon Kim, Min Kyu Effect of Acteoside as a Cell Protector to Produce a Cloned Dog |
title | Effect of Acteoside as a Cell Protector to Produce a Cloned Dog |
title_full | Effect of Acteoside as a Cell Protector to Produce a Cloned Dog |
title_fullStr | Effect of Acteoside as a Cell Protector to Produce a Cloned Dog |
title_full_unstemmed | Effect of Acteoside as a Cell Protector to Produce a Cloned Dog |
title_short | Effect of Acteoside as a Cell Protector to Produce a Cloned Dog |
title_sort | effect of acteoside as a cell protector to produce a cloned dog |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4948914/ https://www.ncbi.nlm.nih.gov/pubmed/27428333 http://dx.doi.org/10.1371/journal.pone.0159330 |
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