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Mitotic noncoding RNA processing promotes kinetochore and spindle assembly in Xenopus

Transcription at the centromere of chromosomes plays an important role in kinetochore assembly in many eukaryotes, and noncoding RNAs contribute to activation of the mitotic kinase Aurora B. However, little is known about how mitotic RNA processing contributes to spindle assembly. We found that inhi...

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Detalles Bibliográficos
Autores principales: Grenfell, Andrew W., Heald, Rebecca, Strzelecka, Magdalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4949451/
https://www.ncbi.nlm.nih.gov/pubmed/27402954
http://dx.doi.org/10.1083/jcb.201604029
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author Grenfell, Andrew W.
Heald, Rebecca
Strzelecka, Magdalena
author_facet Grenfell, Andrew W.
Heald, Rebecca
Strzelecka, Magdalena
author_sort Grenfell, Andrew W.
collection PubMed
description Transcription at the centromere of chromosomes plays an important role in kinetochore assembly in many eukaryotes, and noncoding RNAs contribute to activation of the mitotic kinase Aurora B. However, little is known about how mitotic RNA processing contributes to spindle assembly. We found that inhibition of transcription initiation or RNA splicing, but not translation, leads to spindle defects in Xenopus egg extracts. Spliceosome inhibition resulted in the accumulation of high molecular weight centromeric transcripts, concomitant with decreased recruitment of the centromere and kinetochore proteins CENP-A, CENP-C, and NDC80 to mitotic chromosomes. In addition, blocking transcript synthesis or processing during mitosis caused accumulation of MCAK, a microtubule depolymerase, on the spindle, indicating misregulation of Aurora B. These findings suggest that co-transcriptional recruitment of the RNA processing machinery to nascent mitotic transcripts is an important step in kinetochore and spindle assembly and challenge the idea that RNA processing is globally repressed during mitosis.
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spelling pubmed-49494512017-01-18 Mitotic noncoding RNA processing promotes kinetochore and spindle assembly in Xenopus Grenfell, Andrew W. Heald, Rebecca Strzelecka, Magdalena J Cell Biol Research Articles Transcription at the centromere of chromosomes plays an important role in kinetochore assembly in many eukaryotes, and noncoding RNAs contribute to activation of the mitotic kinase Aurora B. However, little is known about how mitotic RNA processing contributes to spindle assembly. We found that inhibition of transcription initiation or RNA splicing, but not translation, leads to spindle defects in Xenopus egg extracts. Spliceosome inhibition resulted in the accumulation of high molecular weight centromeric transcripts, concomitant with decreased recruitment of the centromere and kinetochore proteins CENP-A, CENP-C, and NDC80 to mitotic chromosomes. In addition, blocking transcript synthesis or processing during mitosis caused accumulation of MCAK, a microtubule depolymerase, on the spindle, indicating misregulation of Aurora B. These findings suggest that co-transcriptional recruitment of the RNA processing machinery to nascent mitotic transcripts is an important step in kinetochore and spindle assembly and challenge the idea that RNA processing is globally repressed during mitosis. The Rockefeller University Press 2016-07-18 /pmc/articles/PMC4949451/ /pubmed/27402954 http://dx.doi.org/10.1083/jcb.201604029 Text en © 2016 Grenfell et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Grenfell, Andrew W.
Heald, Rebecca
Strzelecka, Magdalena
Mitotic noncoding RNA processing promotes kinetochore and spindle assembly in Xenopus
title Mitotic noncoding RNA processing promotes kinetochore and spindle assembly in Xenopus
title_full Mitotic noncoding RNA processing promotes kinetochore and spindle assembly in Xenopus
title_fullStr Mitotic noncoding RNA processing promotes kinetochore and spindle assembly in Xenopus
title_full_unstemmed Mitotic noncoding RNA processing promotes kinetochore and spindle assembly in Xenopus
title_short Mitotic noncoding RNA processing promotes kinetochore and spindle assembly in Xenopus
title_sort mitotic noncoding rna processing promotes kinetochore and spindle assembly in xenopus
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4949451/
https://www.ncbi.nlm.nih.gov/pubmed/27402954
http://dx.doi.org/10.1083/jcb.201604029
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