Cargando…

A Guide to Transient Expression of Membrane Proteins in HEK-293 Cells for Functional Characterization

The human embryonic kidney 293 (HEK-293) cells are commonly used as host for the heterologous expression of membrane proteins not least because they have a high transfection efficiency and faithfully translate and process proteins. In addition, their cell size, morphology and division rate, and low...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ooi, Amanda, Wong, Aloysius, Esau, Luke, Lemtiri-Chlieh, Fouad, Gehring, Chris
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2016
Materias:	Physiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4949579/ https://www.ncbi.nlm.nih.gov/pubmed/27486406 http://dx.doi.org/10.3389/fphys.2016.00300

Ejemplares similares

Mg(2+) is a Missing Link in Plant Cell Ca(2+) Signalling and Homeostasis—A Study on Vicia faba Guard Cells
por: Lemtiri-Chlieh, Fouad, et al.
Publicado: (2020)

The Arabidopsis thaliana K(+)-Uptake Permease 5 (AtKUP5) Contains a Functional Cytosolic Adenylate Cyclase Essential for K(+) Transport
por: Al-Younis, Inas, et al.
Publicado: (2018)

2-AG and anandamide enhance hippocampal long-term potentiation via suppression of inhibition
por: Lemtiri-Chlieh, Fouad, et al.
Publicado: (2022)

Kinetics of the thapsigargin-induced Ca(2+) mobilisation: A quantitative analysis in the HEK-293 cell line
por: Pick, Tillman, et al.
Publicado: (2023)

Predicting Transiently Expressed Protein Yields: Comparison of Transfection Methods in CHO and HEK293
por: Porosk, Ly, et al.
Publicado: (2022)

Effects of Allicin on Late Sodium Current Caused by ΔKPQ-SCN5A Mutation in HEK293 Cells
por: Chen, Yating, et al.
Publicado: (2021)

Characterization of bovine interleukin-2 stably expressed in HEK-293 cells
por: MITOMA, Shuya, et al.
Publicado: (2020)

Influence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells
por: Rajendra, Yashas, et al.
Publicado: (2011)

High-titer expression of recombinant antibodies by transiently transfected HEK 293-6E cell cultures
por: Jäger, Volker, et al.
Publicado: (2015)

Optimization of Insect Odorant Receptor Trafficking and Functional Expression Via Transient Transfection in HEK293 Cells
por: Miazzi, Fabio, et al.
Publicado: (2019)

Cloning, Expression and Characterization of Zebra Fish Ferroportin in Hek 293T Cell Line
por: Rafiee, A, et al.
Publicado: (2012)

High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells
por: Jäger, Volker, et al.
Publicado: (2013)

Effect of PTTG on endogenous gene expression in HEK 293 cells
por: Panguluri, Siva K, et al.
Publicado: (2009)

Characterization of crude Echis carinatus venom-induced cytotoxicity in HEK 293T cells
por: Pierce, Rebecca D, et al.
Publicado: (2011)

Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells
por: Hacker, David L., et al.
Publicado: (2013)

Transient transfection of serum-free suspension HEK 293 cell culture for efficient production of human rFVIII
por: Swiech, Kamilla, et al.
Publicado: (2011)

Enhancing Protein Expression in HEK-293 Cells by Lowering Culture Temperature
por: Lin, Chi-Yen, et al.
Publicado: (2015)

Using HEK293T Expression System to Study Photoactive Plant Cryptochromes
por: Yang, Liang, et al.
Publicado: (2016)

Lack of current observed in HEK293 cells expressing NALCN channels
por: Egan, Jennifer M., et al.
Publicado: (2018)

Characterization of Extracellular Vesicles Secreted in Lentiviral Producing HEK293SF Cell Cultures
por: Do Minh, Aline, et al.
Publicado: (2021)

HEK293S Cells Have Functional Retinoid Processing Machinery
por: Brueggemann, Lioubov I., et al.
Publicado: (2002)

Effects of endotoxin on pacemaker funny current in HEK 293 cells
por: Papaioannou, VP, et al.
Publicado: (2011)

Echinacea-induced cytosolic Ca(2+ )elevation in HEK293
por: Wu, Lankun, et al.
Publicado: (2010)

Analysis of DNA topology of EBV minichromosomes in HEK 293 cells
por: Castán, Alicia, et al.
Publicado: (2017)

HEK293T cell lines defective for O-linked glycosylation
por: Termini, James M., et al.
Publicado: (2017)

Microtubule Integrity Is Associated with the Functional Activity of Mitochondria in HEK293
por: Cho, Min Jeong, et al.
Publicado: (2021)

Development of HEK-293 Cell Lines Constitutively Expressing Flaviviral Antigens for Use in Diagnostics
por: Powers, Jordan A., et al.
Publicado: (2022)

Production of adeno-associated virus (AAV) serotypes by transient transfection of HEK293 cell suspension cultures for gene delivery
por: Chahal, Parminder Singh, et al.
Publicado: (2014)

Cholesterol Down-Regulates BK Channels Stably Expressed in HEK 293 Cells
por: Wu, Wei, et al.
Publicado: (2013)

Allitridi Inhibits Multiple Cardiac Potassium Channels Expressed in HEK 293 Cells
por: Xu, Xiao-Hui, et al.
Publicado: (2012)

Effects of equol on multiple K(+) channels stably expressed in HEK 293 cells
por: Deng, Xiu-Ling, et al.
Publicado: (2017)

Patulin suppresses α(1)-adrenergic receptor expression in HEK293 cells
por: Pillay, Yashodani, et al.
Publicado: (2020)

The CAG promoter maintains high‐level transgene expression in HEK293 cells
por: Dou, Yuanyuan, et al.
Publicado: (2020)

Enhancement of Transgene Expression by Mild Hypothermia Is Promoter Dependent in HEK293 Cells
por: Jang, Min Ho, et al.
Publicado: (2021)

Inducible HEK293 AAV packaging cell lines expressing Rep proteins
por: Jalšić, Lovro, et al.
Publicado: (2023)

Functional properties of cardiac L-type calcium channels transiently expressed in HEK293 cells. Roles of alpha 1 and beta subunits
Publicado: (1995)

CD133 induces tumour-initiating properties in HEK293 cells
por: Canis, Martin, et al.
Publicado: (2012)

Differences in the production of hyperglycosylated IFN alpha in CHO and HEK 293 cells
por: Gugliotta, Agustina, et al.
Publicado: (2013)

Cytotoxicity and genotoxicity caused by yttrium oxide nanoparticles in HEK293 cells
por: Selvaraj, Vellaisamy, et al.
Publicado: (2014)

Cellular signaling pathways modulated by influenza infection in HEK293 cells
por: Manceur, Aziza P, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_5tu2s69rpv31ue880oev4ba40g