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“You produce while I clean up”, a strategy revealed by exoproteomics during Synechococcus–Roseobacter interactions

Most of the energy that is introduced into the oceans by photosynthetic primary producers is in the form of organic matter that then sustains the rest of the food web, from micro to macro‐organisms. However, it is the interactions between phototrophs and heterotrophs that are vital to maintaining th...

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Autores principales: Christie‐Oleza, Joseph A., Scanlan, David J., Armengaud, Jean
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4949626/
https://www.ncbi.nlm.nih.gov/pubmed/25728650
http://dx.doi.org/10.1002/pmic.201400562
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author Christie‐Oleza, Joseph A.
Scanlan, David J.
Armengaud, Jean
author_facet Christie‐Oleza, Joseph A.
Scanlan, David J.
Armengaud, Jean
author_sort Christie‐Oleza, Joseph A.
collection PubMed
description Most of the energy that is introduced into the oceans by photosynthetic primary producers is in the form of organic matter that then sustains the rest of the food web, from micro to macro‐organisms. However, it is the interactions between phototrophs and heterotrophs that are vital to maintaining the nutrient balance of marine microbiomes that ultimately feed these higher trophic levels. The primary produced organic matter is mostly remineralized by heterotrophic microorganisms but, because most of the oceanic dissolved organic matter is in the form of biopolymers, and microbial membrane transport systems operate with molecules <0.6 kDa, it must be hydrolyzed outside the cell before a microorganism can acquire it. As a simili of the marine microbiome, we analyzed, using state‐of‐the‐art proteomics, the exoproteomes obtained from synthetic communities combining specific Roseobacter (Ruegeria pomeroyi DSS‐3, Roseobacter denitrificans OCh114, and Dinoroseobacter shibae DFL‐12) and Synechococcus strains (WH7803 and WH8102). This approach identified the repertoire of hydrolytic enzymes secreted by Roseobacter, opening up the black box of heterotrophic transformation/remineralization of biopolymers generated by marine phytoplankton. As well as highlighting interesting exoenzymes this strategy also allowed us to infer clues on the molecular basis of niche partitioning.
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spelling pubmed-49496262016-07-28 “You produce while I clean up”, a strategy revealed by exoproteomics during Synechococcus–Roseobacter interactions Christie‐Oleza, Joseph A. Scanlan, David J. Armengaud, Jean Proteomics Research Articles Most of the energy that is introduced into the oceans by photosynthetic primary producers is in the form of organic matter that then sustains the rest of the food web, from micro to macro‐organisms. However, it is the interactions between phototrophs and heterotrophs that are vital to maintaining the nutrient balance of marine microbiomes that ultimately feed these higher trophic levels. The primary produced organic matter is mostly remineralized by heterotrophic microorganisms but, because most of the oceanic dissolved organic matter is in the form of biopolymers, and microbial membrane transport systems operate with molecules <0.6 kDa, it must be hydrolyzed outside the cell before a microorganism can acquire it. As a simili of the marine microbiome, we analyzed, using state‐of‐the‐art proteomics, the exoproteomes obtained from synthetic communities combining specific Roseobacter (Ruegeria pomeroyi DSS‐3, Roseobacter denitrificans OCh114, and Dinoroseobacter shibae DFL‐12) and Synechococcus strains (WH7803 and WH8102). This approach identified the repertoire of hydrolytic enzymes secreted by Roseobacter, opening up the black box of heterotrophic transformation/remineralization of biopolymers generated by marine phytoplankton. As well as highlighting interesting exoenzymes this strategy also allowed us to infer clues on the molecular basis of niche partitioning. John Wiley and Sons Inc. 2015-04-21 2015-10 /pmc/articles/PMC4949626/ /pubmed/25728650 http://dx.doi.org/10.1002/pmic.201400562 Text en © 2015 The Authors. PROTEOMICS published by Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Christie‐Oleza, Joseph A.
Scanlan, David J.
Armengaud, Jean
“You produce while I clean up”, a strategy revealed by exoproteomics during Synechococcus–Roseobacter interactions
title “You produce while I clean up”, a strategy revealed by exoproteomics during Synechococcus–Roseobacter interactions
title_full “You produce while I clean up”, a strategy revealed by exoproteomics during Synechococcus–Roseobacter interactions
title_fullStr “You produce while I clean up”, a strategy revealed by exoproteomics during Synechococcus–Roseobacter interactions
title_full_unstemmed “You produce while I clean up”, a strategy revealed by exoproteomics during Synechococcus–Roseobacter interactions
title_short “You produce while I clean up”, a strategy revealed by exoproteomics during Synechococcus–Roseobacter interactions
title_sort “you produce while i clean up”, a strategy revealed by exoproteomics during synechococcus–roseobacter interactions
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4949626/
https://www.ncbi.nlm.nih.gov/pubmed/25728650
http://dx.doi.org/10.1002/pmic.201400562
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