A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (cop-GFP) in Baculovirus Expression System

BACKGROUND: Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. METHODS: The aim of...

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Autores principales: Shokrollahi, Narjes, Shahbazzadeh, Delavar, Pooshang-Bagheri, Kamran, Habibi-Anbouhi, Mahdi, Jahanian-Najafabadi, Ali, Behdani, Mahdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pasteur Institute of Iran 2016
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4949983/
https://www.ncbi.nlm.nih.gov/pubmed/26518237
http://dx.doi.org/10.7508/ibj.2016.03.008
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author Shokrollahi, Narjes
Shahbazzadeh, Delavar
Pooshang-Bagheri, Kamran
Habibi-Anbouhi, Mahdi
Jahanian-Najafabadi, Ali
Behdani, Mahdi
author_facet Shokrollahi, Narjes
Shahbazzadeh, Delavar
Pooshang-Bagheri, Kamran
Habibi-Anbouhi, Mahdi
Jahanian-Najafabadi, Ali
Behdani, Mahdi
author_sort Shokrollahi, Narjes
collection PubMed
description BACKGROUND: Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. METHODS: The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluorescent protein (copGFP). In this light, the resultant vector was constructed and used for transfection of Spodoptera frugiperda cells. RESULTS: Expression of the copGFP protein in insect cells was confirmed by fluorescent microscopy and Western-blot analysis. CONCLUSION: The application of copGFP control bacmid can be considered as an appropriate control for insect cell transfection.
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spelling pubmed-49499832016-07-25 A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (cop-GFP) in Baculovirus Expression System Shokrollahi, Narjes Shahbazzadeh, Delavar Pooshang-Bagheri, Kamran Habibi-Anbouhi, Mahdi Jahanian-Najafabadi, Ali Behdani, Mahdi Iran Biomed J Short Communication BACKGROUND: Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. METHODS: The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluorescent protein (copGFP). In this light, the resultant vector was constructed and used for transfection of Spodoptera frugiperda cells. RESULTS: Expression of the copGFP protein in insect cells was confirmed by fluorescent microscopy and Western-blot analysis. CONCLUSION: The application of copGFP control bacmid can be considered as an appropriate control for insect cell transfection. Pasteur Institute of Iran 2016-07 /pmc/articles/PMC4949983/ /pubmed/26518237 http://dx.doi.org/10.7508/ibj.2016.03.008 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Shokrollahi, Narjes
Shahbazzadeh, Delavar
Pooshang-Bagheri, Kamran
Habibi-Anbouhi, Mahdi
Jahanian-Najafabadi, Ali
Behdani, Mahdi
A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (cop-GFP) in Baculovirus Expression System
title A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (cop-GFP) in Baculovirus Expression System
title_full A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (cop-GFP) in Baculovirus Expression System
title_fullStr A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (cop-GFP) in Baculovirus Expression System
title_full_unstemmed A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (cop-GFP) in Baculovirus Expression System
title_short A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (cop-GFP) in Baculovirus Expression System
title_sort model to study the phenotypic changes of insect cell transfection by copepod super green fluorescent protein (cop-gfp) in baculovirus expression system
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4949983/
https://www.ncbi.nlm.nih.gov/pubmed/26518237
http://dx.doi.org/10.7508/ibj.2016.03.008
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