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Light availability affects stream biofilm bacterial community composition and function, but not diversity
Changes in riparian vegetation or water turbidity and browning in streams alter the local light regime with potential implications for stream biofilms and ecosystem functioning. We experimented with biofilms in microcosms grown under a gradient of light intensities (range: 5–152 μmole photons s(−1) ...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4950016/ https://www.ncbi.nlm.nih.gov/pubmed/26013911 http://dx.doi.org/10.1111/1462-2920.12913 |
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author | Wagner, Karoline Besemer, Katharina Burns, Nancy R. Battin, Tom J. Bengtsson, Mia M. |
author_facet | Wagner, Karoline Besemer, Katharina Burns, Nancy R. Battin, Tom J. Bengtsson, Mia M. |
author_sort | Wagner, Karoline |
collection | PubMed |
description | Changes in riparian vegetation or water turbidity and browning in streams alter the local light regime with potential implications for stream biofilms and ecosystem functioning. We experimented with biofilms in microcosms grown under a gradient of light intensities (range: 5–152 μmole photons s(−1) m(−2)) and combined 454‐pyrosequencing and enzymatic activity assays to evaluate the effects of light on biofilm structure and function. We observed a shift in bacterial community composition along the light gradient, whereas there was no apparent change in alpha diversity. Multifunctionality, based on extracellular enzymes, was highest under high light conditions and decoupled from bacterial diversity. Phenol oxidase activity, involved in the degradation of polyphenolic compounds, was twice as high on average under the lowest compared with the highest light condition. This suggests a shift in reliance of microbial heterotrophs on biofilm phototroph‐derived organic matter under high light availability to more complex organic matter under low light. Furthermore, extracellular enzyme activities correlated with nutrient cycling and community respiration, supporting the link between biofilm structure–function and biogeochemical fluxes in streams. Our findings demonstrate that changes in light availability are likely to have significant impacts on biofilm structure and function, potentially affecting stream ecosystem processes. |
format | Online Article Text |
id | pubmed-4950016 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-49500162016-07-28 Light availability affects stream biofilm bacterial community composition and function, but not diversity Wagner, Karoline Besemer, Katharina Burns, Nancy R. Battin, Tom J. Bengtsson, Mia M. Environ Microbiol Research Articles Changes in riparian vegetation or water turbidity and browning in streams alter the local light regime with potential implications for stream biofilms and ecosystem functioning. We experimented with biofilms in microcosms grown under a gradient of light intensities (range: 5–152 μmole photons s(−1) m(−2)) and combined 454‐pyrosequencing and enzymatic activity assays to evaluate the effects of light on biofilm structure and function. We observed a shift in bacterial community composition along the light gradient, whereas there was no apparent change in alpha diversity. Multifunctionality, based on extracellular enzymes, was highest under high light conditions and decoupled from bacterial diversity. Phenol oxidase activity, involved in the degradation of polyphenolic compounds, was twice as high on average under the lowest compared with the highest light condition. This suggests a shift in reliance of microbial heterotrophs on biofilm phototroph‐derived organic matter under high light availability to more complex organic matter under low light. Furthermore, extracellular enzyme activities correlated with nutrient cycling and community respiration, supporting the link between biofilm structure–function and biogeochemical fluxes in streams. Our findings demonstrate that changes in light availability are likely to have significant impacts on biofilm structure and function, potentially affecting stream ecosystem processes. John Wiley and Sons Inc. 2015-07-22 2015-12 /pmc/articles/PMC4950016/ /pubmed/26013911 http://dx.doi.org/10.1111/1462-2920.12913 Text en © 2015 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Wagner, Karoline Besemer, Katharina Burns, Nancy R. Battin, Tom J. Bengtsson, Mia M. Light availability affects stream biofilm bacterial community composition and function, but not diversity |
title | Light availability affects stream biofilm bacterial community composition and function, but not diversity |
title_full | Light availability affects stream biofilm bacterial community composition and function, but not diversity |
title_fullStr | Light availability affects stream biofilm bacterial community composition and function, but not diversity |
title_full_unstemmed | Light availability affects stream biofilm bacterial community composition and function, but not diversity |
title_short | Light availability affects stream biofilm bacterial community composition and function, but not diversity |
title_sort | light availability affects stream biofilm bacterial community composition and function, but not diversity |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4950016/ https://www.ncbi.nlm.nih.gov/pubmed/26013911 http://dx.doi.org/10.1111/1462-2920.12913 |
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