Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli

Calcium/calmodulin-dependent kinase kinase 2 (CaMKK2) has been implicated in a range of conditions and pathologies from prostate to hepatic cancer. Here, we describe the expression in Escherichia coli and the purification protocol for the following constructs: full-length CaMKK2 in complex with CaM,...

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Autores principales: Gerner, Lisa, Munack, Steffi, Temmerman, Koen, Lawrence-Dörner, Ann-Marie, Besir, Hüseyin, Wilmanns, Matthias, Jensen, Jan Kristian, Thiede, Bernd, Mills, Ian G., Morth, Jens Preben
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4950174/
https://www.ncbi.nlm.nih.gov/pubmed/27508226
http://dx.doi.org/10.1016/j.dib.2016.06.033
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author Gerner, Lisa
Munack, Steffi
Temmerman, Koen
Lawrence-Dörner, Ann-Marie
Besir, Hüseyin
Wilmanns, Matthias
Jensen, Jan Kristian
Thiede, Bernd
Mills, Ian G.
Morth, Jens Preben
author_facet Gerner, Lisa
Munack, Steffi
Temmerman, Koen
Lawrence-Dörner, Ann-Marie
Besir, Hüseyin
Wilmanns, Matthias
Jensen, Jan Kristian
Thiede, Bernd
Mills, Ian G.
Morth, Jens Preben
author_sort Gerner, Lisa
collection PubMed
description Calcium/calmodulin-dependent kinase kinase 2 (CaMKK2) has been implicated in a range of conditions and pathologies from prostate to hepatic cancer. Here, we describe the expression in Escherichia coli and the purification protocol for the following constructs: full-length CaMKK2 in complex with CaM, CaMKK2 ‘apo’, CaMKK2 (165-501) in complex with CaM, and the CaMKK2 F267G mutant. The protocols described have been optimized for maximum yield and purity with minimal purification steps required and the proteins subsequently used to develop a fluorescence-based assay for drug binding to the kinase, “Using the fluorescent properties of STO-609 as a tool to assist structure-function analyses of recombinant CaMKK2” [1].
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spelling pubmed-49501742016-08-09 Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli Gerner, Lisa Munack, Steffi Temmerman, Koen Lawrence-Dörner, Ann-Marie Besir, Hüseyin Wilmanns, Matthias Jensen, Jan Kristian Thiede, Bernd Mills, Ian G. Morth, Jens Preben Data Brief Data Article Calcium/calmodulin-dependent kinase kinase 2 (CaMKK2) has been implicated in a range of conditions and pathologies from prostate to hepatic cancer. Here, we describe the expression in Escherichia coli and the purification protocol for the following constructs: full-length CaMKK2 in complex with CaM, CaMKK2 ‘apo’, CaMKK2 (165-501) in complex with CaM, and the CaMKK2 F267G mutant. The protocols described have been optimized for maximum yield and purity with minimal purification steps required and the proteins subsequently used to develop a fluorescence-based assay for drug binding to the kinase, “Using the fluorescent properties of STO-609 as a tool to assist structure-function analyses of recombinant CaMKK2” [1]. Elsevier 2016-06-29 /pmc/articles/PMC4950174/ /pubmed/27508226 http://dx.doi.org/10.1016/j.dib.2016.06.033 Text en ©e 2, 48149 Münster, Germany. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Gerner, Lisa
Munack, Steffi
Temmerman, Koen
Lawrence-Dörner, Ann-Marie
Besir, Hüseyin
Wilmanns, Matthias
Jensen, Jan Kristian
Thiede, Bernd
Mills, Ian G.
Morth, Jens Preben
Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli
title Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli
title_full Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli
title_fullStr Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli
title_full_unstemmed Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli
title_short Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli
title_sort data for the co-expression and purification of human recombinant camkk2 in complex with calmodulin in escherichia coli
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4950174/
https://www.ncbi.nlm.nih.gov/pubmed/27508226
http://dx.doi.org/10.1016/j.dib.2016.06.033
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