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Human embryonic mesenchymal stem cells participate in differentiation of renal tubular cells in newborn mice

Stem cells are used with increasing success in the treatment of renal tubular injury. However, whether mesenchymal stem cells (MSC) differentiate into renal tubular epithelial cells remains controversial. The aims of the present study were to observe the localization of human embryonic MSCs (hMSCs)...

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Detalles Bibliográficos
Autores principales: Yuan, Li, Liu, Hou-Qi, Wu, Min-Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4950250/
https://www.ncbi.nlm.nih.gov/pubmed/27446255
http://dx.doi.org/10.3892/etm.2016.3383
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author Yuan, Li
Liu, Hou-Qi
Wu, Min-Juan
author_facet Yuan, Li
Liu, Hou-Qi
Wu, Min-Juan
author_sort Yuan, Li
collection PubMed
description Stem cells are used with increasing success in the treatment of renal tubular injury. However, whether mesenchymal stem cells (MSC) differentiate into renal tubular epithelial cells remains controversial. The aims of the present study were to observe the localization of human embryonic MSCs (hMSCs) in the kidneys of newborn mice, and to investigate hMSC differentiation into tubular epithelium. Primary culture hMSCs were derived from 4–7-week-old embryos and labeled with the cell membrane fluorescent dye PKH-26. The degree of apoptosis, cell growth, differentiation and localization of hMSCs with and without this label were then determined using immunohistochemical methods and flow cytometry. hMSCs and PKH26-labeled hMSCs were revealed to differentiate into chondrocytes and adipocytes, and were demonstrated to have similar proliferative capability. In the two cell types, the antigens CD34 and CD45, indicative of hematopoietic lineages, were not expressed; however, the expression of the mesenchymal markers CD29 and CD90 in MSCs, was significantly increased. During a 4-week culture period, laser confocal microscopy revealed that PKH26-labeled hMSCs in the kidneys of newborn mice gradually dispersed. Two weeks after the injection of the PKH26-labeled cells, the percentage of PKH26-labeled hMSCs localized to the renal tubules was 10±2.1%. In conclusion, PKH26 labeling has no effect on hMSC differentiation, proliferation and mesenchymal cell surface features, and hMSCs injected into the kidneys of newborn mice may transform to renal tubule epithelium.
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spelling pubmed-49502502016-07-21 Human embryonic mesenchymal stem cells participate in differentiation of renal tubular cells in newborn mice Yuan, Li Liu, Hou-Qi Wu, Min-Juan Exp Ther Med Articles Stem cells are used with increasing success in the treatment of renal tubular injury. However, whether mesenchymal stem cells (MSC) differentiate into renal tubular epithelial cells remains controversial. The aims of the present study were to observe the localization of human embryonic MSCs (hMSCs) in the kidneys of newborn mice, and to investigate hMSC differentiation into tubular epithelium. Primary culture hMSCs were derived from 4–7-week-old embryos and labeled with the cell membrane fluorescent dye PKH-26. The degree of apoptosis, cell growth, differentiation and localization of hMSCs with and without this label were then determined using immunohistochemical methods and flow cytometry. hMSCs and PKH26-labeled hMSCs were revealed to differentiate into chondrocytes and adipocytes, and were demonstrated to have similar proliferative capability. In the two cell types, the antigens CD34 and CD45, indicative of hematopoietic lineages, were not expressed; however, the expression of the mesenchymal markers CD29 and CD90 in MSCs, was significantly increased. During a 4-week culture period, laser confocal microscopy revealed that PKH26-labeled hMSCs in the kidneys of newborn mice gradually dispersed. Two weeks after the injection of the PKH26-labeled cells, the percentage of PKH26-labeled hMSCs localized to the renal tubules was 10±2.1%. In conclusion, PKH26 labeling has no effect on hMSC differentiation, proliferation and mesenchymal cell surface features, and hMSCs injected into the kidneys of newborn mice may transform to renal tubule epithelium. D.A. Spandidos 2016-08 2016-05-24 /pmc/articles/PMC4950250/ /pubmed/27446255 http://dx.doi.org/10.3892/etm.2016.3383 Text en Copyright: © Yuan et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Yuan, Li
Liu, Hou-Qi
Wu, Min-Juan
Human embryonic mesenchymal stem cells participate in differentiation of renal tubular cells in newborn mice
title Human embryonic mesenchymal stem cells participate in differentiation of renal tubular cells in newborn mice
title_full Human embryonic mesenchymal stem cells participate in differentiation of renal tubular cells in newborn mice
title_fullStr Human embryonic mesenchymal stem cells participate in differentiation of renal tubular cells in newborn mice
title_full_unstemmed Human embryonic mesenchymal stem cells participate in differentiation of renal tubular cells in newborn mice
title_short Human embryonic mesenchymal stem cells participate in differentiation of renal tubular cells in newborn mice
title_sort human embryonic mesenchymal stem cells participate in differentiation of renal tubular cells in newborn mice
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4950250/
https://www.ncbi.nlm.nih.gov/pubmed/27446255
http://dx.doi.org/10.3892/etm.2016.3383
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