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Intestinal Microbiota Promotes Psoriasis-Like Skin Inflammation by Enhancing Th17 Response

Psoriasis is a chronic inflammatory skin disease in which Th17 cells play a crucial role. Since indigenous gut microbiota influences the development and reactivity of immune cells, we analyzed the link among microbiota, T cells and the formation of psoriatic lesions in the imiquimod-induced murine m...

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Autores principales: Zákostelská, Zuzana, Málková, Jana, Klimešová, Klára, Rossmann, Pavel, Hornová, Michaela, Novosádová, Iva, Stehlíková, Zuzana, Kostovčík, Martin, Hudcovic, Tomáš, Štepánková, Renata, Jůzlová, Kateřina, Hercogová, Jana, Tlaskalová-Hogenová, Helena, Kverka, Miloslav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4951142/
https://www.ncbi.nlm.nih.gov/pubmed/27434104
http://dx.doi.org/10.1371/journal.pone.0159539
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author Zákostelská, Zuzana
Málková, Jana
Klimešová, Klára
Rossmann, Pavel
Hornová, Michaela
Novosádová, Iva
Stehlíková, Zuzana
Kostovčík, Martin
Hudcovic, Tomáš
Štepánková, Renata
Jůzlová, Kateřina
Hercogová, Jana
Tlaskalová-Hogenová, Helena
Kverka, Miloslav
author_facet Zákostelská, Zuzana
Málková, Jana
Klimešová, Klára
Rossmann, Pavel
Hornová, Michaela
Novosádová, Iva
Stehlíková, Zuzana
Kostovčík, Martin
Hudcovic, Tomáš
Štepánková, Renata
Jůzlová, Kateřina
Hercogová, Jana
Tlaskalová-Hogenová, Helena
Kverka, Miloslav
author_sort Zákostelská, Zuzana
collection PubMed
description Psoriasis is a chronic inflammatory skin disease in which Th17 cells play a crucial role. Since indigenous gut microbiota influences the development and reactivity of immune cells, we analyzed the link among microbiota, T cells and the formation of psoriatic lesions in the imiquimod-induced murine model of psoriasis. To explore the role of microbiota, we induced skin inflammation in germ-free (GF), broad-spectrum antibiotic (ATB)-treated or conventional (CV) BALB/c and C57BL/6 mice. We found that both mice reared in GF conditions for several generations and CV mice treated with ATB were more resistant to imiquimod-induced skin inflammation than CV mice. The ATB treatment dramatically changed the diversity of gut bacteria, which remained stable after subsequent imiquimod application; ATB treatment resulted in a substantial increase in the order Lactobacillales and a significant decrease in Coriobacteriales and Clostridiales. Moreover, as compared to CV mice, imiquimod induced a lower degree of local and systemic Th17 activation in both GF and ATB-treated mice. These findings suggest that gut microbiota control imiquimod-induced skin inflammation by altering the T cell response.
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spelling pubmed-49511422016-08-08 Intestinal Microbiota Promotes Psoriasis-Like Skin Inflammation by Enhancing Th17 Response Zákostelská, Zuzana Málková, Jana Klimešová, Klára Rossmann, Pavel Hornová, Michaela Novosádová, Iva Stehlíková, Zuzana Kostovčík, Martin Hudcovic, Tomáš Štepánková, Renata Jůzlová, Kateřina Hercogová, Jana Tlaskalová-Hogenová, Helena Kverka, Miloslav PLoS One Research Article Psoriasis is a chronic inflammatory skin disease in which Th17 cells play a crucial role. Since indigenous gut microbiota influences the development and reactivity of immune cells, we analyzed the link among microbiota, T cells and the formation of psoriatic lesions in the imiquimod-induced murine model of psoriasis. To explore the role of microbiota, we induced skin inflammation in germ-free (GF), broad-spectrum antibiotic (ATB)-treated or conventional (CV) BALB/c and C57BL/6 mice. We found that both mice reared in GF conditions for several generations and CV mice treated with ATB were more resistant to imiquimod-induced skin inflammation than CV mice. The ATB treatment dramatically changed the diversity of gut bacteria, which remained stable after subsequent imiquimod application; ATB treatment resulted in a substantial increase in the order Lactobacillales and a significant decrease in Coriobacteriales and Clostridiales. Moreover, as compared to CV mice, imiquimod induced a lower degree of local and systemic Th17 activation in both GF and ATB-treated mice. These findings suggest that gut microbiota control imiquimod-induced skin inflammation by altering the T cell response. Public Library of Science 2016-07-19 /pmc/articles/PMC4951142/ /pubmed/27434104 http://dx.doi.org/10.1371/journal.pone.0159539 Text en © 2016 Zákostelská et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zákostelská, Zuzana
Málková, Jana
Klimešová, Klára
Rossmann, Pavel
Hornová, Michaela
Novosádová, Iva
Stehlíková, Zuzana
Kostovčík, Martin
Hudcovic, Tomáš
Štepánková, Renata
Jůzlová, Kateřina
Hercogová, Jana
Tlaskalová-Hogenová, Helena
Kverka, Miloslav
Intestinal Microbiota Promotes Psoriasis-Like Skin Inflammation by Enhancing Th17 Response
title Intestinal Microbiota Promotes Psoriasis-Like Skin Inflammation by Enhancing Th17 Response
title_full Intestinal Microbiota Promotes Psoriasis-Like Skin Inflammation by Enhancing Th17 Response
title_fullStr Intestinal Microbiota Promotes Psoriasis-Like Skin Inflammation by Enhancing Th17 Response
title_full_unstemmed Intestinal Microbiota Promotes Psoriasis-Like Skin Inflammation by Enhancing Th17 Response
title_short Intestinal Microbiota Promotes Psoriasis-Like Skin Inflammation by Enhancing Th17 Response
title_sort intestinal microbiota promotes psoriasis-like skin inflammation by enhancing th17 response
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4951142/
https://www.ncbi.nlm.nih.gov/pubmed/27434104
http://dx.doi.org/10.1371/journal.pone.0159539
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