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Up-regulation of serotonin receptor 2B mRNA and protein in the peri-infarcted area of aged rats and stroke patients

Despite the fact that a high proportion of elderly stroke patients develop mood disorders, the mechanisms underlying late-onset neuropsychiatric and neurocognitive symptoms have so far received little attention in the field of neurobiology. In rodents, aged animals display depressive symptoms follow...

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Autores principales: Buga, Ana-Maria, Ciobanu, Ovidiu, Bădescu, George Mihai, Bogdan, Catalin, Weston, Ria, Slevin, Mark, Di Napoli, Mario, Popa-Wagner, Aurel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4951222/
https://www.ncbi.nlm.nih.gov/pubmed/27013593
http://dx.doi.org/10.18632/oncotarget.8277
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author Buga, Ana-Maria
Ciobanu, Ovidiu
Bădescu, George Mihai
Bogdan, Catalin
Weston, Ria
Slevin, Mark
Di Napoli, Mario
Popa-Wagner, Aurel
author_facet Buga, Ana-Maria
Ciobanu, Ovidiu
Bădescu, George Mihai
Bogdan, Catalin
Weston, Ria
Slevin, Mark
Di Napoli, Mario
Popa-Wagner, Aurel
author_sort Buga, Ana-Maria
collection PubMed
description Despite the fact that a high proportion of elderly stroke patients develop mood disorders, the mechanisms underlying late-onset neuropsychiatric and neurocognitive symptoms have so far received little attention in the field of neurobiology. In rodents, aged animals display depressive symptoms following stroke, whereas young animals recover fairly well. This finding has prompted us to investigate the expression of serotonin receptors 2A and 2B, which are directly linked to depression, in the brains of aged and young rats following stroke. Although the development of the infarct was more rapid in aged rats in the first 3 days after stroke, by day 14 the cortical infarcts were similar in size in both age groups i.e. 45% of total cortical volume in young rats and 55.7% in aged rats. We also found that the expression of serotonin receptor type B mRNA was markedly increased in the perilesional area of aged rats as compared to the younger counterparts. Furthermore, histologically, HTR2B protein expression in degenerating neurons was closely associated with activated microglia both in aged rats and human subjects. Treatment with fluoxetine attenuated the expression of Htr2B mRNA, stimulated post-stroke neurogenesis in the subventricular zone and was associated with an improved anhedonic behavior and an increased activity in the forced swim test in aged animals. We hypothesize that HTR2B expression in the infarcted territory may render degenerating neurons susceptible to attack by activated microglia and thus aggravate the consequences of stroke.
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spelling pubmed-49512222016-07-21 Up-regulation of serotonin receptor 2B mRNA and protein in the peri-infarcted area of aged rats and stroke patients Buga, Ana-Maria Ciobanu, Ovidiu Bădescu, George Mihai Bogdan, Catalin Weston, Ria Slevin, Mark Di Napoli, Mario Popa-Wagner, Aurel Oncotarget Research Paper: Gerotarget (Focus on Aging) Despite the fact that a high proportion of elderly stroke patients develop mood disorders, the mechanisms underlying late-onset neuropsychiatric and neurocognitive symptoms have so far received little attention in the field of neurobiology. In rodents, aged animals display depressive symptoms following stroke, whereas young animals recover fairly well. This finding has prompted us to investigate the expression of serotonin receptors 2A and 2B, which are directly linked to depression, in the brains of aged and young rats following stroke. Although the development of the infarct was more rapid in aged rats in the first 3 days after stroke, by day 14 the cortical infarcts were similar in size in both age groups i.e. 45% of total cortical volume in young rats and 55.7% in aged rats. We also found that the expression of serotonin receptor type B mRNA was markedly increased in the perilesional area of aged rats as compared to the younger counterparts. Furthermore, histologically, HTR2B protein expression in degenerating neurons was closely associated with activated microglia both in aged rats and human subjects. Treatment with fluoxetine attenuated the expression of Htr2B mRNA, stimulated post-stroke neurogenesis in the subventricular zone and was associated with an improved anhedonic behavior and an increased activity in the forced swim test in aged animals. We hypothesize that HTR2B expression in the infarcted territory may render degenerating neurons susceptible to attack by activated microglia and thus aggravate the consequences of stroke. Impact Journals LLC 2016-03-22 /pmc/articles/PMC4951222/ /pubmed/27013593 http://dx.doi.org/10.18632/oncotarget.8277 Text en Copyright: © 2016 Buga et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper: Gerotarget (Focus on Aging)
Buga, Ana-Maria
Ciobanu, Ovidiu
Bădescu, George Mihai
Bogdan, Catalin
Weston, Ria
Slevin, Mark
Di Napoli, Mario
Popa-Wagner, Aurel
Up-regulation of serotonin receptor 2B mRNA and protein in the peri-infarcted area of aged rats and stroke patients
title Up-regulation of serotonin receptor 2B mRNA and protein in the peri-infarcted area of aged rats and stroke patients
title_full Up-regulation of serotonin receptor 2B mRNA and protein in the peri-infarcted area of aged rats and stroke patients
title_fullStr Up-regulation of serotonin receptor 2B mRNA and protein in the peri-infarcted area of aged rats and stroke patients
title_full_unstemmed Up-regulation of serotonin receptor 2B mRNA and protein in the peri-infarcted area of aged rats and stroke patients
title_short Up-regulation of serotonin receptor 2B mRNA and protein in the peri-infarcted area of aged rats and stroke patients
title_sort up-regulation of serotonin receptor 2b mrna and protein in the peri-infarcted area of aged rats and stroke patients
topic Research Paper: Gerotarget (Focus on Aging)
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4951222/
https://www.ncbi.nlm.nih.gov/pubmed/27013593
http://dx.doi.org/10.18632/oncotarget.8277
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