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Study of chondrogenic potential of stem cells in co-culture with chondrons

OBJECTIVE(S): Three-dimensional biomimetic scaffolds have widespread applications in biomedical tissue engineering due to similarity of their nanofibrous architecture to native extracellular matrix. Co-culture system has stimulatory effect on chondrogenesis of adult mesenchymal stem cells. This work...

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Autores principales: Nikpou, Parisa, Nejad, Daryoush Mohammad, Shafaei, Hajar, Roshangar, Leila, Samadi, Nasser, Navali, Amir Mohammad, Sadegpour, Ali Reza, Shanehbandi, Dariush, Rad, Jafar Soleimani
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4951603/
https://www.ncbi.nlm.nih.gov/pubmed/27482345
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author Nikpou, Parisa
Nejad, Daryoush Mohammad
Shafaei, Hajar
Roshangar, Leila
Samadi, Nasser
Navali, Amir Mohammad
Sadegpour, Ali Reza
Shanehbandi, Dariush
Rad, Jafar Soleimani
author_facet Nikpou, Parisa
Nejad, Daryoush Mohammad
Shafaei, Hajar
Roshangar, Leila
Samadi, Nasser
Navali, Amir Mohammad
Sadegpour, Ali Reza
Shanehbandi, Dariush
Rad, Jafar Soleimani
author_sort Nikpou, Parisa
collection PubMed
description OBJECTIVE(S): Three-dimensional biomimetic scaffolds have widespread applications in biomedical tissue engineering due to similarity of their nanofibrous architecture to native extracellular matrix. Co-culture system has stimulatory effect on chondrogenesis of adult mesenchymal stem cells. This work presents a co-culture strategy using human articular chondrons and adipose-derived stem cells (ASCs) from infrapatellar fat pad (IPFP) for cartilage tissue production. MATERIALS AND METHODS: Isolated stem cells were characterized by flowcytometry. Electrospun and polycaprolactone (PCL) scaffolds (900 nm fiber diameter) was obtained from Bon Yakhteh (Tehran-Iran) and human infrapatellar fat pad-derived stem cells (IPFP-ASCs) were seeded on them. IPFP-ASCs on scaffolds were co-cultured with articular chondrons using transwell. After 21 day, chondrogenic differentiation of stem cell was evaluated by determining the genes expression of collagen2, aggrecan and Indian hedgehog using real-time RT-PCR. RESULTS: Genes expression of collagen2, aggrecan by IPFP-ASCs did not alter significantly in comparison with control group. Howevers, expression of Indian hedgehog decreased significantly compared to control group (P< 0.05). CONCLUSION: These findings indicate that chondrons obtained from osteoarthritic articular cartilage did not stimulate chondrogenic differentiation of IPFP-ASCs in co-culture.
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spelling pubmed-49516032016-08-01 Study of chondrogenic potential of stem cells in co-culture with chondrons Nikpou, Parisa Nejad, Daryoush Mohammad Shafaei, Hajar Roshangar, Leila Samadi, Nasser Navali, Amir Mohammad Sadegpour, Ali Reza Shanehbandi, Dariush Rad, Jafar Soleimani Iran J Basic Med Sci Original Article OBJECTIVE(S): Three-dimensional biomimetic scaffolds have widespread applications in biomedical tissue engineering due to similarity of their nanofibrous architecture to native extracellular matrix. Co-culture system has stimulatory effect on chondrogenesis of adult mesenchymal stem cells. This work presents a co-culture strategy using human articular chondrons and adipose-derived stem cells (ASCs) from infrapatellar fat pad (IPFP) for cartilage tissue production. MATERIALS AND METHODS: Isolated stem cells were characterized by flowcytometry. Electrospun and polycaprolactone (PCL) scaffolds (900 nm fiber diameter) was obtained from Bon Yakhteh (Tehran-Iran) and human infrapatellar fat pad-derived stem cells (IPFP-ASCs) were seeded on them. IPFP-ASCs on scaffolds were co-cultured with articular chondrons using transwell. After 21 day, chondrogenic differentiation of stem cell was evaluated by determining the genes expression of collagen2, aggrecan and Indian hedgehog using real-time RT-PCR. RESULTS: Genes expression of collagen2, aggrecan by IPFP-ASCs did not alter significantly in comparison with control group. Howevers, expression of Indian hedgehog decreased significantly compared to control group (P< 0.05). CONCLUSION: These findings indicate that chondrons obtained from osteoarthritic articular cartilage did not stimulate chondrogenic differentiation of IPFP-ASCs in co-culture. Mashhad University of Medical Sciences 2016-06 /pmc/articles/PMC4951603/ /pubmed/27482345 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Nikpou, Parisa
Nejad, Daryoush Mohammad
Shafaei, Hajar
Roshangar, Leila
Samadi, Nasser
Navali, Amir Mohammad
Sadegpour, Ali Reza
Shanehbandi, Dariush
Rad, Jafar Soleimani
Study of chondrogenic potential of stem cells in co-culture with chondrons
title Study of chondrogenic potential of stem cells in co-culture with chondrons
title_full Study of chondrogenic potential of stem cells in co-culture with chondrons
title_fullStr Study of chondrogenic potential of stem cells in co-culture with chondrons
title_full_unstemmed Study of chondrogenic potential of stem cells in co-culture with chondrons
title_short Study of chondrogenic potential of stem cells in co-culture with chondrons
title_sort study of chondrogenic potential of stem cells in co-culture with chondrons
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4951603/
https://www.ncbi.nlm.nih.gov/pubmed/27482345
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