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Genomic content typifying a prevalent clade of bovine mastitis-associated Escherichia coli
E. coli represents a heterogeneous population with capabilities to cause disease in several anatomical sites. Among sites that can be colonised is the bovine mammary gland (udder) and a distinct class of mammary pathogenic E. coli (MPEC) has been proposed. MPEC are the principle causative agents of...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4951805/ https://www.ncbi.nlm.nih.gov/pubmed/27436046 http://dx.doi.org/10.1038/srep30115 |
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author | Goldstone, Robert J. Harris, Susan Smith, David G. E. |
author_facet | Goldstone, Robert J. Harris, Susan Smith, David G. E. |
author_sort | Goldstone, Robert J. |
collection | PubMed |
description | E. coli represents a heterogeneous population with capabilities to cause disease in several anatomical sites. Among sites that can be colonised is the bovine mammary gland (udder) and a distinct class of mammary pathogenic E. coli (MPEC) has been proposed. MPEC are the principle causative agents of bovine mastitis in well-managed dairy farms, costing producers in the European Union an estimated €2 billion per year. Despite the economic impact, and the threat this disease presents to small and medium sized dairy farmers, the factors which mediate the ability for E. coli to thrive in bovine mammary tissue remain poorly elucidated. Strains belonging to E. coli phylogroup A are most frequently isolated from mastitis. In this paper, we apply a population level genomic analysis to this group of E. coli to uncover genomic signatures of mammary infectivity. Through a robust statistical analysis, we show that not all strains of E. coli are equally likely to cause mastitis, and those that do possess specific gene content that may promote their adaptation and survival in the bovine udder. Through a pan-genomic analysis, we identify just three genetic loci which are ubiquitous in MPEC, but appear dispensable for E. coli from other niches. |
format | Online Article Text |
id | pubmed-4951805 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49518052016-07-26 Genomic content typifying a prevalent clade of bovine mastitis-associated Escherichia coli Goldstone, Robert J. Harris, Susan Smith, David G. E. Sci Rep Article E. coli represents a heterogeneous population with capabilities to cause disease in several anatomical sites. Among sites that can be colonised is the bovine mammary gland (udder) and a distinct class of mammary pathogenic E. coli (MPEC) has been proposed. MPEC are the principle causative agents of bovine mastitis in well-managed dairy farms, costing producers in the European Union an estimated €2 billion per year. Despite the economic impact, and the threat this disease presents to small and medium sized dairy farmers, the factors which mediate the ability for E. coli to thrive in bovine mammary tissue remain poorly elucidated. Strains belonging to E. coli phylogroup A are most frequently isolated from mastitis. In this paper, we apply a population level genomic analysis to this group of E. coli to uncover genomic signatures of mammary infectivity. Through a robust statistical analysis, we show that not all strains of E. coli are equally likely to cause mastitis, and those that do possess specific gene content that may promote their adaptation and survival in the bovine udder. Through a pan-genomic analysis, we identify just three genetic loci which are ubiquitous in MPEC, but appear dispensable for E. coli from other niches. Nature Publishing Group 2016-07-20 /pmc/articles/PMC4951805/ /pubmed/27436046 http://dx.doi.org/10.1038/srep30115 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Goldstone, Robert J. Harris, Susan Smith, David G. E. Genomic content typifying a prevalent clade of bovine mastitis-associated Escherichia coli |
title | Genomic content typifying a prevalent clade of bovine mastitis-associated Escherichia coli |
title_full | Genomic content typifying a prevalent clade of bovine mastitis-associated Escherichia coli |
title_fullStr | Genomic content typifying a prevalent clade of bovine mastitis-associated Escherichia coli |
title_full_unstemmed | Genomic content typifying a prevalent clade of bovine mastitis-associated Escherichia coli |
title_short | Genomic content typifying a prevalent clade of bovine mastitis-associated Escherichia coli |
title_sort | genomic content typifying a prevalent clade of bovine mastitis-associated escherichia coli |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4951805/ https://www.ncbi.nlm.nih.gov/pubmed/27436046 http://dx.doi.org/10.1038/srep30115 |
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