Comparative proteomic analysis provides novel insight into the interaction between resistant vs susceptible tomato cultivars and TYLCV infection

BACKGROUND: Tomato yellow leaf curl virus (TYLCV) is a member of the family Geminiviridae, genus Begomovirus. The virus is a widespread plant virus that causes important economic losses in tomatoes. Genetic engineering strategies have increasingly been adopted to improve the resistance of tomatoes to TYLCV. RESULTS: In this study, a proteomic approach was used to investigate the molecular mechanisms involved in tomato leaf defense against TYLCV infection. Proteins extracted from leaves of resistant tomato cultivar ‘Zheza-301’ and susceptible cultivar ‘Jinpeng-1’ after TYLCV infection were analyzed using two-dimensional gel electrophoresis. Eighty-six differentially expressed proteins were identified and classified into seven groups based on their functions. For several of the proteins, including CDC48, CHI and HSC70, expression patterns measured using quantitative real-time PCR differed from the results of the proteomic analysis. A putative interaction network between tomato leaves and TYLCV infection provides us with important information about the cellular activities that are involved in the response to TYLCV infection. CONCLUSIONS: We conducted a comparative proteomic study of TYLCV infection in resistant and susceptible tomato cultivars. The proteins identified in our work show a variety of functions and expression patterns in the process of tomato–TYLCV interaction, and these results contribute to our understanding of the mechanism underlying TYLCV resistance in tomatoes at the protein level. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-016-0819-z) contains supplementary material, which is available to authorized users.