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Sida tuberculata (Malvaceae): a study based on development of extractive system and in silico and in vitro properties
Sida tuberculata (Malvaceae) is a medicinal plant traditionally used in Brazil as an antimicrobial and anti-inflammatory agent. Here, we aimed to investigate the different extractive techniques on phytochemical parameters, as well as to evaluate the toxicity and antioxidant capacity of S. tuberculat...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Associação Brasileira de Divulgação Científica
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4954737/ https://www.ncbi.nlm.nih.gov/pubmed/27409335 http://dx.doi.org/10.1590/1414-431X20165282 |
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author | da Rosa, H.S. Salgueiro, A.C.F. Colpo, A.Z.C. Paula, F.R. Mendez, A.S.L. Folmer, V. |
author_facet | da Rosa, H.S. Salgueiro, A.C.F. Colpo, A.Z.C. Paula, F.R. Mendez, A.S.L. Folmer, V. |
author_sort | da Rosa, H.S. |
collection | PubMed |
description | Sida tuberculata (Malvaceae) is a medicinal plant traditionally used in Brazil as an antimicrobial and anti-inflammatory agent. Here, we aimed to investigate the different extractive techniques on phytochemical parameters, as well as to evaluate the toxicity and antioxidant capacity of S. tuberculata extracts using in silico and in vitro models. Therefore, in order to determine the dry residue content and the main compound 20-hydroxyecdysone (20E) concentration, extracts from leaves and roots were prepared testing ethanol and water in different proportions. Extracts were then assessed by Artemia salina lethality test, and toxicity prediction of 20E was estimated. Antioxidant activity was performed by DPPH and ABTS radical scavenger assays, ferric reducing power assay, nitrogen derivative scavenger, deoxyribose degradation, and TBARS assays. HPLC evaluation detected 20E as main compound in leaves and roots. Percolation method showed the highest concentrations of 20E (0.134 and 0.096 mg/mL of extract for leaves and roots, respectively). All crude extracts presented low toxic potential on A. salina (LD(50) >1000 µg/mL). The computational evaluation of 20E showed a low toxicity prediction. For in vitro antioxidant tests, hydroethanolic extracts of leaves were most effective compared to roots. In addition, hydroethanolic extracts presented a higher IC(50) antioxidant than aqueous extracts. TBARS formation was prevented by leaves hydroethanolic extract from 0.015 and 0.03 mg/mL and for roots from 0.03 and 0.3 mg/mL on egg yolk and rat tissue, respectively (P<0.05). These findings suggest that S. tuberculata extracts are a considerable source of ecdysteroids and possesses a significant antioxidant property with low toxic potential. |
format | Online Article Text |
id | pubmed-4954737 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Associação Brasileira de Divulgação Científica |
record_format | MEDLINE/PubMed |
spelling | pubmed-49547372016-08-03 Sida tuberculata (Malvaceae): a study based on development of extractive system and in silico and in vitro properties da Rosa, H.S. Salgueiro, A.C.F. Colpo, A.Z.C. Paula, F.R. Mendez, A.S.L. Folmer, V. Braz J Med Biol Res Biomedical Sciences Sida tuberculata (Malvaceae) is a medicinal plant traditionally used in Brazil as an antimicrobial and anti-inflammatory agent. Here, we aimed to investigate the different extractive techniques on phytochemical parameters, as well as to evaluate the toxicity and antioxidant capacity of S. tuberculata extracts using in silico and in vitro models. Therefore, in order to determine the dry residue content and the main compound 20-hydroxyecdysone (20E) concentration, extracts from leaves and roots were prepared testing ethanol and water in different proportions. Extracts were then assessed by Artemia salina lethality test, and toxicity prediction of 20E was estimated. Antioxidant activity was performed by DPPH and ABTS radical scavenger assays, ferric reducing power assay, nitrogen derivative scavenger, deoxyribose degradation, and TBARS assays. HPLC evaluation detected 20E as main compound in leaves and roots. Percolation method showed the highest concentrations of 20E (0.134 and 0.096 mg/mL of extract for leaves and roots, respectively). All crude extracts presented low toxic potential on A. salina (LD(50) >1000 µg/mL). The computational evaluation of 20E showed a low toxicity prediction. For in vitro antioxidant tests, hydroethanolic extracts of leaves were most effective compared to roots. In addition, hydroethanolic extracts presented a higher IC(50) antioxidant than aqueous extracts. TBARS formation was prevented by leaves hydroethanolic extract from 0.015 and 0.03 mg/mL and for roots from 0.03 and 0.3 mg/mL on egg yolk and rat tissue, respectively (P<0.05). These findings suggest that S. tuberculata extracts are a considerable source of ecdysteroids and possesses a significant antioxidant property with low toxic potential. Associação Brasileira de Divulgação Científica 2016-07-11 /pmc/articles/PMC4954737/ /pubmed/27409335 http://dx.doi.org/10.1590/1414-431X20165282 Text en http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License |
spellingShingle | Biomedical Sciences da Rosa, H.S. Salgueiro, A.C.F. Colpo, A.Z.C. Paula, F.R. Mendez, A.S.L. Folmer, V. Sida tuberculata (Malvaceae): a study based on development of extractive system and in silico and in vitro properties |
title |
Sida tuberculata (Malvaceae): a study based on development of extractive system and in silico and in vitro properties |
title_full |
Sida tuberculata (Malvaceae): a study based on development of extractive system and in silico and in vitro properties |
title_fullStr |
Sida tuberculata (Malvaceae): a study based on development of extractive system and in silico and in vitro properties |
title_full_unstemmed |
Sida tuberculata (Malvaceae): a study based on development of extractive system and in silico and in vitro properties |
title_short |
Sida tuberculata (Malvaceae): a study based on development of extractive system and in silico and in vitro properties |
title_sort | sida tuberculata (malvaceae): a study based on development of extractive system and in silico and in vitro properties |
topic | Biomedical Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4954737/ https://www.ncbi.nlm.nih.gov/pubmed/27409335 http://dx.doi.org/10.1590/1414-431X20165282 |
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