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Genetic dissection of the Transcription Factor code controlling serial specification of muscle identities in Drosophila

Each Drosophila muscle is seeded by one Founder Cell issued from terminal division of a Progenitor Cell (PC). Muscle identity reflects the expression by each PC of a specific combination of identity Transcription Factors (iTFs). Sequential emergence of several PCs at the same position raised the que...

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Autores principales: Dubois, Laurence, Frendo, Jean-Louis, Chanut-Delalande, Hélène, Crozatier, Michèle, Vincent, Alain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4954755/
https://www.ncbi.nlm.nih.gov/pubmed/27438571
http://dx.doi.org/10.7554/eLife.14979
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author Dubois, Laurence
Frendo, Jean-Louis
Chanut-Delalande, Hélène
Crozatier, Michèle
Vincent, Alain
author_facet Dubois, Laurence
Frendo, Jean-Louis
Chanut-Delalande, Hélène
Crozatier, Michèle
Vincent, Alain
author_sort Dubois, Laurence
collection PubMed
description Each Drosophila muscle is seeded by one Founder Cell issued from terminal division of a Progenitor Cell (PC). Muscle identity reflects the expression by each PC of a specific combination of identity Transcription Factors (iTFs). Sequential emergence of several PCs at the same position raised the question of how developmental time controlled muscle identity. Here, we identified roles of Anterior Open and ETS domain lacking in controlling PC birth time and Eyes absent, No Ocelli, and Sine oculis in specifying PC identity. The windows of transcription of these and other TFs in wild type and mutant embryos, revealed a cascade of regulation integrating time and space, feed-forward loops and use of alternative transcription start sites. These data provide a dynamic view of the transcriptional control of muscle identity in Drosophila and an extended framework for studying interactions between general myogenic factors and iTFs in evolutionary diversification of muscle shapes. DOI: http://dx.doi.org/10.7554/eLife.14979.001
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spelling pubmed-49547552016-07-21 Genetic dissection of the Transcription Factor code controlling serial specification of muscle identities in Drosophila Dubois, Laurence Frendo, Jean-Louis Chanut-Delalande, Hélène Crozatier, Michèle Vincent, Alain eLife Developmental Biology and Stem Cells Each Drosophila muscle is seeded by one Founder Cell issued from terminal division of a Progenitor Cell (PC). Muscle identity reflects the expression by each PC of a specific combination of identity Transcription Factors (iTFs). Sequential emergence of several PCs at the same position raised the question of how developmental time controlled muscle identity. Here, we identified roles of Anterior Open and ETS domain lacking in controlling PC birth time and Eyes absent, No Ocelli, and Sine oculis in specifying PC identity. The windows of transcription of these and other TFs in wild type and mutant embryos, revealed a cascade of regulation integrating time and space, feed-forward loops and use of alternative transcription start sites. These data provide a dynamic view of the transcriptional control of muscle identity in Drosophila and an extended framework for studying interactions between general myogenic factors and iTFs in evolutionary diversification of muscle shapes. DOI: http://dx.doi.org/10.7554/eLife.14979.001 eLife Sciences Publications, Ltd 2016-07-20 /pmc/articles/PMC4954755/ /pubmed/27438571 http://dx.doi.org/10.7554/eLife.14979 Text en © 2016, Dubois et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology and Stem Cells
Dubois, Laurence
Frendo, Jean-Louis
Chanut-Delalande, Hélène
Crozatier, Michèle
Vincent, Alain
Genetic dissection of the Transcription Factor code controlling serial specification of muscle identities in Drosophila
title Genetic dissection of the Transcription Factor code controlling serial specification of muscle identities in Drosophila
title_full Genetic dissection of the Transcription Factor code controlling serial specification of muscle identities in Drosophila
title_fullStr Genetic dissection of the Transcription Factor code controlling serial specification of muscle identities in Drosophila
title_full_unstemmed Genetic dissection of the Transcription Factor code controlling serial specification of muscle identities in Drosophila
title_short Genetic dissection of the Transcription Factor code controlling serial specification of muscle identities in Drosophila
title_sort genetic dissection of the transcription factor code controlling serial specification of muscle identities in drosophila
topic Developmental Biology and Stem Cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4954755/
https://www.ncbi.nlm.nih.gov/pubmed/27438571
http://dx.doi.org/10.7554/eLife.14979
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