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Potential role of punicalagin against oxidative stress induced testicular damage

Punicalagin is isolated from pomegranate and widely used for the treatment of different diseases in Chinese traditional medicine. This study aimed to evaluate the effect of Punicalagin (purity ≥98%) on oxidative stress induced testicular damage and its effect on fertility. We detected the antioxidan...

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Autores principales: Rao, Faiza, Tian, Hui, Li, Wenqing, Hung, Helong, Sun, Fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4955191/
https://www.ncbi.nlm.nih.gov/pubmed/26763544
http://dx.doi.org/10.4103/1008-682X.168792
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author Rao, Faiza
Tian, Hui
Li, Wenqing
Hung, Helong
Sun, Fei
author_facet Rao, Faiza
Tian, Hui
Li, Wenqing
Hung, Helong
Sun, Fei
author_sort Rao, Faiza
collection PubMed
description Punicalagin is isolated from pomegranate and widely used for the treatment of different diseases in Chinese traditional medicine. This study aimed to evaluate the effect of Punicalagin (purity ≥98%) on oxidative stress induced testicular damage and its effect on fertility. We detected the antioxidant potential of punicalagin in lipopolysaccharide (LPS) induced oxidative stress damage in testes, also tried to uncover the boosting fertility effect of Punicalagin (PU) against oxidative stress-induced infertility. Results demonstrated that 9 mg kg(−1) for 7 days treatment significantly decreases LPS induced oxidative damage in testes and nitric oxide production. The administration of oxidative stress resulted in a significant reduction in testes antioxidants GSH, T-SOD, and CAT raised LPO, but treatment with punicalagin for 7 days increased antioxidant defense GSH, T-SOD, and CAT by the end of the experiment and reduced LPO level as well. PU also significantly activates Nrf2, which is involved in regulation of antioxidant defense systems. Hence, the present research categorically elucidates the protective effect of punicalagin against LPS induced oxidative stress induced perturbation in the process of spermatogenesis and significantly increased sperm health and number. Moreover, fertility success significantly decreased in LPS-injected mice compared to controls. Mice injected with LPS had fertility indices of 12.5%, while others treated with a combination of PU + LPS exhibited 75% indices. By promoting fertility and eliminating oxidative stress and inflammation, PU may be a useful nutrient for the treatment of infertility.
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spelling pubmed-49551912016-07-26 Potential role of punicalagin against oxidative stress induced testicular damage Rao, Faiza Tian, Hui Li, Wenqing Hung, Helong Sun, Fei Asian J Androl Original Article Punicalagin is isolated from pomegranate and widely used for the treatment of different diseases in Chinese traditional medicine. This study aimed to evaluate the effect of Punicalagin (purity ≥98%) on oxidative stress induced testicular damage and its effect on fertility. We detected the antioxidant potential of punicalagin in lipopolysaccharide (LPS) induced oxidative stress damage in testes, also tried to uncover the boosting fertility effect of Punicalagin (PU) against oxidative stress-induced infertility. Results demonstrated that 9 mg kg(−1) for 7 days treatment significantly decreases LPS induced oxidative damage in testes and nitric oxide production. The administration of oxidative stress resulted in a significant reduction in testes antioxidants GSH, T-SOD, and CAT raised LPO, but treatment with punicalagin for 7 days increased antioxidant defense GSH, T-SOD, and CAT by the end of the experiment and reduced LPO level as well. PU also significantly activates Nrf2, which is involved in regulation of antioxidant defense systems. Hence, the present research categorically elucidates the protective effect of punicalagin against LPS induced oxidative stress induced perturbation in the process of spermatogenesis and significantly increased sperm health and number. Moreover, fertility success significantly decreased in LPS-injected mice compared to controls. Mice injected with LPS had fertility indices of 12.5%, while others treated with a combination of PU + LPS exhibited 75% indices. By promoting fertility and eliminating oxidative stress and inflammation, PU may be a useful nutrient for the treatment of infertility. Medknow Publications & Media Pvt Ltd 2016 2016-01-12 /pmc/articles/PMC4955191/ /pubmed/26763544 http://dx.doi.org/10.4103/1008-682X.168792 Text en Copyright: © Asian Journal of Andrology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Rao, Faiza
Tian, Hui
Li, Wenqing
Hung, Helong
Sun, Fei
Potential role of punicalagin against oxidative stress induced testicular damage
title Potential role of punicalagin against oxidative stress induced testicular damage
title_full Potential role of punicalagin against oxidative stress induced testicular damage
title_fullStr Potential role of punicalagin against oxidative stress induced testicular damage
title_full_unstemmed Potential role of punicalagin against oxidative stress induced testicular damage
title_short Potential role of punicalagin against oxidative stress induced testicular damage
title_sort potential role of punicalagin against oxidative stress induced testicular damage
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4955191/
https://www.ncbi.nlm.nih.gov/pubmed/26763544
http://dx.doi.org/10.4103/1008-682X.168792
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