Cargando…
Minimum inhibitory concentration of vancomycin to methicillin resistant Staphylococcus aureus isolated from different clinical samples at a tertiary care hospital in Nepal
BACKGROUND: Methicillin resistant Staphylococcus aureus (MRSA) has evolved as a serious threat to public health. It has capability to cause infections not only in health care settings but also in community. Due to the multidrug resistance shown by MRSA, there are limited treatment options for the in...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4955258/ https://www.ncbi.nlm.nih.gov/pubmed/27446531 http://dx.doi.org/10.1186/s13756-016-0126-3 |
_version_ | 1782443920476602368 |
---|---|
author | Kshetry, Arjun Ojha Pant, Narayan Dutt Bhandari, Raju Khatri, Sabita Shrestha, Krishma Laxmi Upadhaya, Shambhu Kumar Poudel, Asia Lekhak, Binod Raghubanshi, Bijendra R. |
author_facet | Kshetry, Arjun Ojha Pant, Narayan Dutt Bhandari, Raju Khatri, Sabita Shrestha, Krishma Laxmi Upadhaya, Shambhu Kumar Poudel, Asia Lekhak, Binod Raghubanshi, Bijendra R. |
author_sort | Kshetry, Arjun Ojha |
collection | PubMed |
description | BACKGROUND: Methicillin resistant Staphylococcus aureus (MRSA) has evolved as a serious threat to public health. It has capability to cause infections not only in health care settings but also in community. Due to the multidrug resistance shown by MRSA, there are limited treatment options for the infections caused by this superbug. Vancomycin is used as the drug of choice for the treatment of infections caused by MRSA. Different studies from all around the world have documented the emergence of strains of S. aureus those are intermediate sensitive or resistant to vancomycin. And recently, there have been reports of reduced susceptibility of MRSA to vancomycin, from Nepal also. So the main purpose of this study was to determine the minimum inhibitory concentration (MIC) of vancomycin to methicillin resistant S. aureus isolated from different clinical specimens. METHODS: Total 125 strains of S. aureus isolated from different clinical samples at KIST Medical College and Teaching Hospital, Lalitpur, Nepal from Nov 2012 to June 2013, were subjected to MRSA detection by cefoxitin disc diffusion method. The minimum inhibitory concentrations of vancomycin to confirmed MRSA strains were determined by agar dilution method. Yellow colored colonies in mannitol salt agar, which were gram positive cocci, catalase positive and coagulase positive were confirmed to be S. aureus. RESULTS: Among, total 125 S. aureus strains isolated; 47(37.6%) were MRSA. Minimum inhibitory concentrations of vancomycin to the strains of MRSA ranged from 0.125 μg/ml to 1 μg/ml. CONCLUSION: From our findings we concluded that the rate of isolation of MRSA among all the strains of S. aureus isolated from clinical samples was very high. However, none of the MRSA strains were found to be vancomycin intermediate-sensitive or vancomycin-resistant. |
format | Online Article Text |
id | pubmed-4955258 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-49552582016-07-22 Minimum inhibitory concentration of vancomycin to methicillin resistant Staphylococcus aureus isolated from different clinical samples at a tertiary care hospital in Nepal Kshetry, Arjun Ojha Pant, Narayan Dutt Bhandari, Raju Khatri, Sabita Shrestha, Krishma Laxmi Upadhaya, Shambhu Kumar Poudel, Asia Lekhak, Binod Raghubanshi, Bijendra R. Antimicrob Resist Infect Control Research BACKGROUND: Methicillin resistant Staphylococcus aureus (MRSA) has evolved as a serious threat to public health. It has capability to cause infections not only in health care settings but also in community. Due to the multidrug resistance shown by MRSA, there are limited treatment options for the infections caused by this superbug. Vancomycin is used as the drug of choice for the treatment of infections caused by MRSA. Different studies from all around the world have documented the emergence of strains of S. aureus those are intermediate sensitive or resistant to vancomycin. And recently, there have been reports of reduced susceptibility of MRSA to vancomycin, from Nepal also. So the main purpose of this study was to determine the minimum inhibitory concentration (MIC) of vancomycin to methicillin resistant S. aureus isolated from different clinical specimens. METHODS: Total 125 strains of S. aureus isolated from different clinical samples at KIST Medical College and Teaching Hospital, Lalitpur, Nepal from Nov 2012 to June 2013, were subjected to MRSA detection by cefoxitin disc diffusion method. The minimum inhibitory concentrations of vancomycin to confirmed MRSA strains were determined by agar dilution method. Yellow colored colonies in mannitol salt agar, which were gram positive cocci, catalase positive and coagulase positive were confirmed to be S. aureus. RESULTS: Among, total 125 S. aureus strains isolated; 47(37.6%) were MRSA. Minimum inhibitory concentrations of vancomycin to the strains of MRSA ranged from 0.125 μg/ml to 1 μg/ml. CONCLUSION: From our findings we concluded that the rate of isolation of MRSA among all the strains of S. aureus isolated from clinical samples was very high. However, none of the MRSA strains were found to be vancomycin intermediate-sensitive or vancomycin-resistant. BioMed Central 2016-07-21 /pmc/articles/PMC4955258/ /pubmed/27446531 http://dx.doi.org/10.1186/s13756-016-0126-3 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Kshetry, Arjun Ojha Pant, Narayan Dutt Bhandari, Raju Khatri, Sabita Shrestha, Krishma Laxmi Upadhaya, Shambhu Kumar Poudel, Asia Lekhak, Binod Raghubanshi, Bijendra R. Minimum inhibitory concentration of vancomycin to methicillin resistant Staphylococcus aureus isolated from different clinical samples at a tertiary care hospital in Nepal |
title | Minimum inhibitory concentration of vancomycin to methicillin resistant Staphylococcus aureus isolated from different clinical samples at a tertiary care hospital in Nepal |
title_full | Minimum inhibitory concentration of vancomycin to methicillin resistant Staphylococcus aureus isolated from different clinical samples at a tertiary care hospital in Nepal |
title_fullStr | Minimum inhibitory concentration of vancomycin to methicillin resistant Staphylococcus aureus isolated from different clinical samples at a tertiary care hospital in Nepal |
title_full_unstemmed | Minimum inhibitory concentration of vancomycin to methicillin resistant Staphylococcus aureus isolated from different clinical samples at a tertiary care hospital in Nepal |
title_short | Minimum inhibitory concentration of vancomycin to methicillin resistant Staphylococcus aureus isolated from different clinical samples at a tertiary care hospital in Nepal |
title_sort | minimum inhibitory concentration of vancomycin to methicillin resistant staphylococcus aureus isolated from different clinical samples at a tertiary care hospital in nepal |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4955258/ https://www.ncbi.nlm.nih.gov/pubmed/27446531 http://dx.doi.org/10.1186/s13756-016-0126-3 |
work_keys_str_mv | AT kshetryarjunojha minimuminhibitoryconcentrationofvancomycintomethicillinresistantstaphylococcusaureusisolatedfromdifferentclinicalsamplesatatertiarycarehospitalinnepal AT pantnarayandutt minimuminhibitoryconcentrationofvancomycintomethicillinresistantstaphylococcusaureusisolatedfromdifferentclinicalsamplesatatertiarycarehospitalinnepal AT bhandariraju minimuminhibitoryconcentrationofvancomycintomethicillinresistantstaphylococcusaureusisolatedfromdifferentclinicalsamplesatatertiarycarehospitalinnepal AT khatrisabita minimuminhibitoryconcentrationofvancomycintomethicillinresistantstaphylococcusaureusisolatedfromdifferentclinicalsamplesatatertiarycarehospitalinnepal AT shresthakrishmalaxmi minimuminhibitoryconcentrationofvancomycintomethicillinresistantstaphylococcusaureusisolatedfromdifferentclinicalsamplesatatertiarycarehospitalinnepal AT upadhayashambhukumar minimuminhibitoryconcentrationofvancomycintomethicillinresistantstaphylococcusaureusisolatedfromdifferentclinicalsamplesatatertiarycarehospitalinnepal AT poudelasia minimuminhibitoryconcentrationofvancomycintomethicillinresistantstaphylococcusaureusisolatedfromdifferentclinicalsamplesatatertiarycarehospitalinnepal AT lekhakbinod minimuminhibitoryconcentrationofvancomycintomethicillinresistantstaphylococcusaureusisolatedfromdifferentclinicalsamplesatatertiarycarehospitalinnepal AT raghubanshibijendrar minimuminhibitoryconcentrationofvancomycintomethicillinresistantstaphylococcusaureusisolatedfromdifferentclinicalsamplesatatertiarycarehospitalinnepal |