A Myocardial Slice Culture Model Reveals Alpha-1A-Adrenergic Receptor Signaling in the Human Heart

The authors used 52 nonfailing and failing human hearts to develop a simple, high throughput left ventricular myocardial slice model that is stable by ATP and viability assays for at least 3 days. The model supports studies of signaling, contraction, and viral transduction. They use the model to sho...

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Detalles Bibliográficos
Autores principales: Thomas, R. Croft, Singh, Abhishek, Cowley, Patrick M., Myagmar, Bat-Erdene, Montgomery, Megan D., Swigart, Philip M., De Marco, Teresa, Baker, Anthony J., Simpson, Paul C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
NOVEL TRANSLATIONAL METHOD
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4955869/
https://www.ncbi.nlm.nih.gov/pubmed/27453955
http://dx.doi.org/10.1016/j.jacbts.2016.03.005
Descripción
Sumario:The authors used 52 nonfailing and failing human hearts to develop a simple, high throughput left ventricular myocardial slice model that is stable by ATP and viability assays for at least 3 days. The model supports studies of signaling, contraction, and viral transduction. They use the model to show for the first time that the alpha-1A-adrenergic receptor, which is present at very low abundance in the human myocardium, activates cardioprotective ERK with nanomolar EC50 in failing heart slices and stimulates a positive inotropic effect. This model should be useful for translational studies, to test whether molecules discovered in basic experiments are functional in the human heart.

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