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Dopaminergic Modulation of Excitatory Transmission in the Anterior Cingulate Cortex of Adult Mice
Dopamine (DA) possesses potent neuromodulatory properties in the central nervous system. In the anterior cingulate cortex, α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors (AMPAR) are key ion channels in mediating nerve injury induced long-term potentiation (LTP) and chronic pain phen...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4955973/ https://www.ncbi.nlm.nih.gov/pubmed/27317578 http://dx.doi.org/10.1177/1744806916648153 |
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author | Darvish-Ghane, Soroush Yamanaka, Manabu Zhuo, Min |
author_facet | Darvish-Ghane, Soroush Yamanaka, Manabu Zhuo, Min |
author_sort | Darvish-Ghane, Soroush |
collection | PubMed |
description | Dopamine (DA) possesses potent neuromodulatory properties in the central nervous system. In the anterior cingulate cortex, α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors (AMPAR) are key ion channels in mediating nerve injury induced long-term potentiation (LTP) and chronic pain phenotype. In the present study, we reported the effects of DA on glutamate mediated excitatory post-synaptic currents (EPSCs) in pyramidal neurons of layer II/III of the ACC in adult mice. Bath application of DA (50 μM) caused a significant, rapid and reversible inhibition of evoked EPSCs (eEPSC). This inhibitory effect is dose-related and was absent in lower concentration of DA (5 μM). Furthermore, selective postsynaptic application of GDP-β-S (1.6 mM) in the internal solution completely abolished the inhibitory effects of DA (50 μM). We also investigated modulation of spontaneous EPSCs (sEPSCs) and TTX sensitive, miniature EPSCs (mEPSCs) by DA. Our results indicated mixed effects of potentiation and inhibition of frequency and amplitude for sEPSCs and mEPSCs. Furthermore, high doses of SCH23390 (100 μM) and sulpiride (100 μM) revealed that, inhibition of eEPSCs is mediated by postsynaptic D2-receptors (D2R). Our finding posits a pre- and postsynaptic mode of pyramidal neuron EPSC modulation in mice ACC by DA. |
format | Online Article Text |
id | pubmed-4955973 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-49559732016-08-12 Dopaminergic Modulation of Excitatory Transmission in the Anterior Cingulate Cortex of Adult Mice Darvish-Ghane, Soroush Yamanaka, Manabu Zhuo, Min Mol Pain Research Article Dopamine (DA) possesses potent neuromodulatory properties in the central nervous system. In the anterior cingulate cortex, α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors (AMPAR) are key ion channels in mediating nerve injury induced long-term potentiation (LTP) and chronic pain phenotype. In the present study, we reported the effects of DA on glutamate mediated excitatory post-synaptic currents (EPSCs) in pyramidal neurons of layer II/III of the ACC in adult mice. Bath application of DA (50 μM) caused a significant, rapid and reversible inhibition of evoked EPSCs (eEPSC). This inhibitory effect is dose-related and was absent in lower concentration of DA (5 μM). Furthermore, selective postsynaptic application of GDP-β-S (1.6 mM) in the internal solution completely abolished the inhibitory effects of DA (50 μM). We also investigated modulation of spontaneous EPSCs (sEPSCs) and TTX sensitive, miniature EPSCs (mEPSCs) by DA. Our results indicated mixed effects of potentiation and inhibition of frequency and amplitude for sEPSCs and mEPSCs. Furthermore, high doses of SCH23390 (100 μM) and sulpiride (100 μM) revealed that, inhibition of eEPSCs is mediated by postsynaptic D2-receptors (D2R). Our finding posits a pre- and postsynaptic mode of pyramidal neuron EPSC modulation in mice ACC by DA. SAGE Publications 2016-06-17 /pmc/articles/PMC4955973/ /pubmed/27317578 http://dx.doi.org/10.1177/1744806916648153 Text en © The Author(s) 2016 http://creativecommons.org/licenses/by-nc/3.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Research Article Darvish-Ghane, Soroush Yamanaka, Manabu Zhuo, Min Dopaminergic Modulation of Excitatory Transmission in the Anterior Cingulate Cortex of Adult Mice |
title | Dopaminergic Modulation of Excitatory Transmission in the Anterior Cingulate Cortex of Adult Mice |
title_full | Dopaminergic Modulation of Excitatory Transmission in the Anterior Cingulate Cortex of Adult Mice |
title_fullStr | Dopaminergic Modulation of Excitatory Transmission in the Anterior Cingulate Cortex of Adult Mice |
title_full_unstemmed | Dopaminergic Modulation of Excitatory Transmission in the Anterior Cingulate Cortex of Adult Mice |
title_short | Dopaminergic Modulation of Excitatory Transmission in the Anterior Cingulate Cortex of Adult Mice |
title_sort | dopaminergic modulation of excitatory transmission in the anterior cingulate cortex of adult mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4955973/ https://www.ncbi.nlm.nih.gov/pubmed/27317578 http://dx.doi.org/10.1177/1744806916648153 |
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